A Basin Method of Nut Tree Culture

Nut tree culture in Iowa is confined almost entirely too selected and grafted varieties of the black walnut, Juglan nigra L. This tree is one of the dominants of the Elm-Ash-Walnut Associes of the Deciduous Forest Formation of eastern United States. Its native habitat is the upper flood-plains of rivers, creeks and even intermittent streams. In spite of the fact that Iowa is near the western limit of its range, the walnut thrives in the rich alluvial soil of well drained but moist floodplains throughout the state and attains a size almost as great as it does farther east near the center of its range

The Canadian Neuromuscular Disease Registry 2010-2019: A Decade of Facilitating Clinical Research Througha Nationwide, Pan-NeuromuscularDisease Registry

We report the recruitment activities and outcomes of a multi-disease neuromuscular patient registry in Canada. The Canadian Neuromuscular Disease Registry (CNDR) registers individuals across Canada with a confirmed diagnosis of a neuromuscular disease. Diagnosis and contact information are collected across all diseases and detailed prospective data is collected for 5 specific diseases: Amyotrophic Lateral Sclerosis (ALS), Duchenne Muscular Dystrophy (DMD), Myotonic Dystrophy (DM), Limb Girdle Muscular Dystrophy (LGMD), and Spinal Muscular Atrophy (SMA). Since 2010, the CNDR has registered 4306 patients (1154 pediatric and 3148 adult) with 91 different neuromuscular diagnoses and has facilitated 125 projects (73 academic, 3 not-for-profit, 3 government, and 46 commercial) using registry data. In conclusion, the CNDR is an effective and productive pan-neuromuscular registry that has successfully facilitated a substantial number of studies over the past 10 years

Whole Genome Sequencing Identified a 16 Kilobase Deletion on ECA13 Associated with Distichiasis in Friesian Horses

BACKGROUND: Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of the Meibomian glands of the eyelid, has been reported in Friesian horses. These misplaced cilia can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to corneal fibrosis, or, if secondary infection occurs, may lead to loss of the eye. Friesian horses represent the vast majority of reported cases of equine distichiasis, and as the breed is known to be affected with inherited monogenic disorders, this condition was hypothesized to be a simply inherited Mendelian trait. RESULTS: A genome wide association study (GWAS) was performed using the Axiom 670 k Equine Genotyping array (MNEc670k) utilizing 14 cases and 38 controls phenotyped for distichiasis. An additive single locus mixed linear model (EMMAX) approach identified a 1.83 Mb locus on ECA5 and a 1.34 Mb locus on ECA13 that reached genome-wide significance (pcorrected = 0.016 and 0.032, respectively). Only the locus on ECA13 withstood replication testing (p = 1.6 × 10− 5, cases: n = 5 and controls: n = 37). A 371 kb run of homozygosity (ROH) on ECA13 was found in 13 of the 14 cases, providing evidence for a recessive mode of inheritance. Haplotype analysis (hapQTL) narrowed the region of association on ECA13 to 163 kb. Whole-genome sequencing data from 3 cases and 2 controls identified a 16 kb deletion within the ECA13 associated haplotype (ECA13:g.178714_195130del). Functional annotation data supports a tissue-specific regulatory role of this locus. This deletion was associated with distichiasis, as 18 of the 19 cases were homozygous (p = 4.8 × 10− 13). Genotyping the deletion in 955 horses from 54 different breeds identified the deletion in only 11 non-Friesians, all of which were carriers, suggesting that this could be causal for this Friesian disorder. CONCLUSIONS: This study identified a 16 kb deletion on ECA13 in an intergenic region that was associated with distichiasis in Friesian horses. Further functional analysis in relevant tissues from cases and controls will help to clarify the precise role of this deletion in normal and abnormal eyelash development and investigate the hypothesis of incomplete penetrance

A National Spinal Muscular Atrophy Registry for Real-World Evidence.

BACKGROUND: Spinal muscular atrophy (SMA) is a devastating rare disease that affects individuals regardless of ethnicity, gender, and age. The first-approved disease-modifying therapy for SMA, nusinursen, was approved by Health Canada, as well as by American and European regulatory agencies following positive clinical trial outcomes. The trials were conducted in a narrow pediatric population defined by age, severity, and genotype. Broad approval of therapy necessitates close follow-up of potential rare adverse events and effectiveness in the larger real-world population. METHODS: The Canadian Neuromuscular Disease Registry (CNDR) undertook an iterative multi-stakeholder process to expand the existing SMA dataset to capture items relevant to patient outcomes in a post-marketing environment. The CNDR SMA expanded registry is a longitudinal, prospective, observational study of patients with SMA in Canada designed to evaluate the safety and effectiveness of novel therapies and provide practical information unattainable in trials. RESULTS: The consensus expanded dataset includes items that address therapy effectiveness and safety and is collected in a multicenter, prospective, observational study, including SMA patients regardless of therapeutic status. The expanded dataset is aligned with global datasets to facilitate collaboration. Additionally, consensus dataset development aimed to standardize appropriate outcome measures across the network and broader Canadian community. Prospective outcome studies, data use, and analyses are independent of the funding partner. CONCLUSION: Prospective outcome data collected will provide results on safety and effectiveness in a post-therapy approval era. These data are essential to inform improvements in care and access to therapy for all SMA patients

Organic foods : a comparison of selection, perception, and consumption habits of college students in Indiana and Oregon : an honors thesis (HONRS 499)

The purpose of this study was to compare perceptions about the benefits and the motivation for selecting and consuming organic foods between students in two different geographic locations (e.g., Ball State University (BSU) in Muncie, Indiana and University of Oregon (U of 0) in Eugene, Oregon).A total of 183 adults completed this survey—76 students from BSU and 107 students from U of O. The results indicated that Oregon students were much more likely to choose organic foods than Indiana students on a "daily" or "weekly" basis. Students in Oregon were more likely to feel that artificial colorings and flavors, hormones, and antibiotics in meat were harmful to humans. Artificial colorings and flavors were also viewed as detrimental to the environment by Oregon students. Organic foods were viewed as healthier, safer, better for the environment, better tasting, and more in harmony with political and religious views to students surveyed in Oregon. More Indiana students stated that a higher cost was a reason why they did not select organic foods. Overall, Oregon students were much more likely to consume organic foods, and they were more likely to feel that the consumption of organic foods was beneficial to both humans and the environment.Thesis (B.S.)Honors Colleg

Correlates of Physical Activity Among Hispanic Adults

Health behaviors of minority populations, including Hispanics, are important from a public health perspective because this subpopulation is growing and health behaviors of this subgroup are understudied. Physical activity is a component of healthy lifestyles and Hispanics have been shown to be less active than are Caucasians. It will be necessary to know correlates of physical activity to enhance the physical activity of this group. Recently, the importance of environmental and cultural factors has been recognized as correlates of physical activity behavior. The purpose of this study was to identify environmental and cultural correlates of physical activity among Hispanic adults