11 research outputs found
Biodegradability assessment of complex chemical mixtures using a carbon balance approach
International audienc
Development of a biosensor for on-line detection of tributyltin with a recombinant bioluminescent Escherichia coli strain
Potential of Raman Spectroscopy To Monitor Arsenic Toxicity on Bacteria: Insights toward Multiparametric Bioassays
In the field of toxicological bioassays,
the latest progress in
Raman spectroscopy opens new research perspectives on a fast method
of observing metabolic responses against toxic agents. This technique
offers a multiparametric approach, providing an overview of the physiological
changes that are caused by pollutants. However, physiological spectral
fingerprints require complex chemometric methods for proper analysis.
In this study, particular attention has been given to the elaboration
of an âaberrant spectraâ detection strategy to highlight
the effects of arsenic on the bacteria Escherichia
coli. This strategy significantly improved spectra
classification, consistent with a doseâresponse effect of the
four tested concentrations of the metal. Indeed, the correct classification
score of the spectra increased from 88 to more than 99%. The exposure
time effect has also been investigated. The fine analysis of Raman
spectroscopy fingerprints enabled the design of different âspectral
signaturesâ, highlighting early and late effects of arsenic
on bacteria. The observed variations are in agreement with the expected
toxicity and encourage the use of Raman spectroscopy for toxic element
detection
Potential of Raman Spectroscopy To Monitor Arsenic Toxicity on Bacteria: Insights toward Multiparametric Bioassays
Characterization by Raman Spectroscopy of Gold Surface Functionalization and Immuno-specific protein binding for Biosensor applications.
International audienc