Molecular mechanisms of extracellular adenine nucleotides-mediated inhibition of human Cd4+ T lymphocytes activation

We have previously reported that ATPγS, a slowly hydrolyzed analog of ATP, inhibits the activation of human CD4+ T lymphocytes by anti-CD3 and anti-CD28 mAb. In this report we have partially characterized the signaling mechanisms involved in this immunosuppressive effect. ATPγS had no inhibitory effect on CD4+ T-cell activation induced by PMA and anti-CD28, indicating that it acts proximally to the TCR. It had no effect on the calcium rise induced by CD3/CD28 stimulation, but inhibited the phosphorylation of three kinases, ERK2, p38 MAPK and PKB, that play a key role in the activation of T cells. The receptor involved in these actions remains unidentified

Purinergic signalling and immune cells

This review article provides a historical perspective on the role of purinergic signalling in the regulation of various subsets of immune cells from early discoveries to current understanding. It is now recognised that adenosine 5'-triphosphate (ATP) and other nucleotides are released from cells following stress or injury. They can act on virtually all subsets of immune cells through a spectrum of P2X ligand-gated ion channels and G protein-coupled P2Y receptors. Furthermore, ATP is rapidly degraded into adenosine by ectonucleotidases such as CD39 and CD73, and adenosine exerts additional regulatory effects through its own receptors. The resulting effect ranges from stimulation to tolerance depending on the amount and time courses of nucleotides released, and the balance between ATP and adenosine. This review identifies the various receptors involved in the different subsets of immune cells and their effects on the function of these cells

Fast and wideband supercontinuum absorption spectroscopy in the mid-IR range

International audienceWe report on our new test bench dedicated to Supercontinuum Absorption Spectroscopy in the mid-infrared (3.3 µm). It delivers fast (<0.1 s) and wideband spectra (200 nm) at 0.8 cm-1 resolution. Gas concentrations are retrieved using a DOAS-inspired algorithm

Low loss microstructured chalcogenide fibers for large non linear effects at 1995 nm.

International audienceMicrostructured optical fibers (MOFs) are traditionally prepared using the stack and draw technique. In order to avoid the interfaces problems observed in chalcogenide glasses, we have developed a new casting method to prepare the chalcogenide preform. This method allows to reach optical losses around 0.4 dB/m at 1.55 µm and less than 0.05 dB/m in the mid IR. Various As(38)Se(62) chalcogenide microstructured fibers have been prepared in order to combine large non linear index of these glasses with the mode control offered by MOF structures. Small core fibers have been drawn to enhance the non linearities. In one of these, three Stokes order have been generated by Raman scattering in a suspended core MOF pumped at 1995 nm

Mid-infrared strong spectral broadening in microstructured tapered chalcogenide AsSe fiber

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Nonlinear effects above 2 µm in chalcogenide suspended core microstructured optical fibers: modeling and experiments

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Fourth-order cascaded Raman shift in AsSe chalcogenide suspended-core fiber pumped at 2 μm

Cascaded Raman wavelength shifting up to the fourth order ranging from 2092 to 2450 nm is demonstrated using a nanosecond pump at 1995 nm in a low-loss As38Se62 suspended-core microstructured fiber. These four Stokes shifts are obtained with a low peak power of 11 W, and only 3 W are required to obtain three shifts. The Raman gain coefficient for the fiber is estimated to (1.6±0.5)×10−11 m/W at 1995 nm. The positions and the amplitudes of the Raman peaks are well reproduced by the numerical simulations of the nonlinear propagation

Recent developments in chalcogenide PCF

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Microstructured Optical Fibers from As2S3 Glass for Fiber Optics Sources in the MIR range

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Nonlinear effects generation in suspended core chalcogenide fibre

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