68 research outputs found

    Biofilm and the role of the ica operon and aap in Staphylococcus epidermidis isolates causing neurosurgical meningitis.

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    Fifty-five Staphylococcus epidermidis isolates, classified as contaminants or causing device-related meningitis, from external ventricular drain (EVD) and non-EVD cerebrospinal fluid specimens were characterized. Thirty-three of 42 (78.6%) meningitis isolates were PCR-positive for ica and aap, known determinants of polysaccharide- and protein-mediated biofilm production, whereas five of 13 (38.5%) contaminants were ica- and aap-negative; 71.4% of meningitis isolates and 84.6% of contaminants produced biofilm. ica+aap+ meningitis isolates produced more biofilm than ica+aap- isolates (p 0.0020). ica+aap- isolates did not produce more biofilm than ica-aap+ isolates (p 0.4368). Apparently, ica and aap are associated with biofilm production in S. epidermidis device-related meningitis isolates

    Anthropocene environmental change in an internationally important oligotrophic catchment on the Atlantic seaboard of western Europe

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    Oligotrophic catchments with short spatey streams, upland lakes and peaty soils characterise northwest European Atlantic coastal regions. These catchments are important biodiversity refuges, particularly for sensitive diadromous fish populations but are subject to changes in land use and land management practices associated with afforestation, agriculture and rural development. Quantification of the degree of catchment degradation resulting from such anthropogenic impacts is often limited by a lack of long-term baseline data in what are generally relatively isolated, poorly studied catchments. This research uses a combination of palaeolimnological (radiometrically-dated variations in sedimentary geochemical elements, pollen, diatoms and remains of cladocera), census, and instrumental data, along with hindcast estimates to quantify environmental changes and their aquatic impacts since the late 19th century. The most likely drivers of any change are also identified. Results confirm an aquatic biotic response (phyto- and zooplankton) to soil erosion and nutrient enrichment associated with the onset of commercial conifer afforestation, effects that were subsequently enhanced as a result of increased overgrazing in the catchment and, possibly, climate warming. The implications for the health of aquatic resources in the catchment are discussedEnvironmental Protection Agency in Ireland (ILLUMINATE 2005-W-MS-40, P.McGinnity was supported by the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme

    Genetic stock identification of Atlantic salmon (Salmo salar) populations in the southern part of the European range

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    notes: PMCID: PMC2882343© 2010 Griffiths et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Anadromous migratory fish species such as Atlantic salmon (Salmo salar) have significant economic, cultural and ecological importance, but present a complex case for management and conservation due to the range of their migration. Atlantic salmon exist in rivers across the North Atlantic, returning to their river of birth with a high degree of accuracy; however, despite continuing efforts and improvements in in-river conservation, they are in steep decline across their range. Salmon from rivers across Europe migrate along similar routes, where they have, historically, been subject to commercial netting. This mixed stock exploitation has the potential to devastate weak and declining populations where they are exploited indiscriminately. Despite various tagging and marking studies, the effect of marine exploitation and the marine element of the salmon lifecycle in general, remain the "black-box" of salmon management. In a number of Pacific salmonid species and in several regions within the range of the Atlantic salmon, genetic stock identification and mixed stock analysis have been used successfully to quantify exploitation rates and identify the natal origins of fish outside their home waters - to date this has not been attempted for Atlantic salmon in the south of their European range.European Union INTERREG IIIB programme (Atlantic Salmon Arc Project [ASAP], Project No. 040)

    The response of North Atlantic diadromous fish to multiple stressors including land use change: a multidecadal study

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    This is a author-produced PDF of a 'Just in' article published in Canadian Journal of Fisheries and Aquatic Sciences following peer review. The definitive publisher-authenticated version 'The response of North Atlantic diadromous fish to multiple stressors including land use change: a multidecadal study Elvira de Eyto, Catherine Dalton, Mary M Dillane, Eleanor Jennings, Philip McGinnity, Barry O'Dwyer, Russell Poole, Ger G Rogan, David Taylor Canadian Journal of Fisheries and Aquatic Sciences, Published on the web 19 May 2016, 10.1139/cjfas-2015-0450' is available from DOI 10.1139/cjfas-2015-0450'Reduction of freshwater habitat quality due to land use change can have significant impacts on diadromous fish. Partitioning this impact from other potential drivers, such as changing marine conditions and climate, is hampered by a lack of long term datasets. Here, four decades of data were used to assess the impact of land use change on Salmo salar L. and anadromous Salmo trutta L. in the Burrishoole catchment, Ireland, one of the few index sites for diadromous fish in the North Atlantic. Land use change was found to have no significant impact on the freshwater survival of either salmon or trout. However, climate impacted significantly on the survival of salmon and trout in freshwater, with poor survival in years with wetter warmer winters, coinciding with positive North Atlantic Oscillation values. Additionally, cold springs were associated with higher survival in trout. The addition of hatchery salmon into the salmon spawning cohort coincided with low freshwater survival. Our results highlight the necessity for a broad ecosystem approach in any conservation effort of these species

    Modelling and characterisation of a ultrasound-actuated needle for improved visibility in ultrasound-guided regional anaesthesia and tissue biopsy

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    AbstractClear needle visualisation is recognised as an unmet need for ultrasound guided percutaneous needle procedures including regional anaesthesia and tissue biopsy. With inadequate needle visibility, these procedures may result in serious complications or a failed operation. This paper reports analysis of the modal behaviour of a previously proposed ultrasound-actuated needle configuration, which may overcome this problem by improving needle visibility in colour Doppler imaging. It uses a piezoelectric transducer to actuate longitudinal resonant modes in needles (outer diameter 0.8–1.2mm, length>65mm). The factors that affect the needle’s vibration mode are identified, including the needle length, the transducer’s resonance frequency and the gripping position. Their effects are investigated using finite element modelling, with the conclusions validated experimentally. The actuated needle was inserted into porcine tissue up to 30mm depth and its visibility was observed under colour Doppler imaging. The piezoelectric transducer is able to generate longitudinal vibration with peak-to-peak amplitude up to 4μm at the needle tip with an actuating voltage of 20Vpp. Actuated in longitudinal vibration modes (distal mode at 27.6kHz and transducer mode at 42.2kHz) with a drive amplitude of 12–14Vpp, a 120mm needle is delineated as a coloured line in colour Doppler images, with both needle tip and shaft visualised. The improved needle visibility is maintained while the needle is advanced into the tissue, thus allowing tracking of the needle position in real time. Moreover, the needle tip is highlighted by strong coloured artefacts around the actuated needle generated by its flexural vibration. A limitation of the technique is that the transducer mode requires needles of specific lengths so that the needle’s resonance frequency matches the transducer. This may restrict the choice of needle lengths in clinical applications

    Synchrony in catchment stream colour levels is driven by both local and regional climate

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    Streams draining upland catchments carry large quantities of carbon from terrestrial stocks to downstream freshwater and marine ecosystems. Here it either enters long-term storage in sediments or enters the atmosphere as gaseous carbon through a combination of biotic and abiotic processes. There are, however, increasing concerns over the long-term stability of terrestrial carbon stores in blanket peatland catchments as a result of anthropogenic pressures and climate change. We analysed sub-annual and inter-annual changes in river water colour (a reliable proxy measurement of dissolved organic carbon; DOC) using 6 years of weekly data, from 2011 to 2016. This time-series dataset was gathered from three contiguous river sub-catchments, the Black, the Glenamong and the Srahrevagh, in a blanket peatland catchment system in western Ireland, and it was used to identify the drivers that best explained observed temporal change in river colour. The data were also used to estimate annual DOC loads from each catchment. General additive mixed modelling was used to identify the principle environmental drivers of water colour in the rivers, while wavelet cross-correlation analysis was used to identify common frequencies in correlations. At 130&thinsp;mg&thinsp;Pt&thinsp;Co&thinsp;L−1, the mean colour levels in the Srahrevagh (the sub-catchment with lowest rainfall and higher forest cover) were almost 50&thinsp;% higher than those from the Black and Glenamong, at 95 and 84&thinsp;mg&thinsp;Pt&thinsp;Co&thinsp;L−1 respectively. The decomposition of the colour datasets revealed similar multi-annual, annual and event-based (random component) trends, illustrating that environmental drivers operated synchronously at each of these temporal scales. For both the Black and its nested Srahrevagh catchment, three variables (soil temperature, soil moisture deficit, SMD, and the weekly North Atlantic Oscillation, NAO) combined to explain 54&thinsp;% and 58&thinsp;% of the deviance in colour respectively. In the Glenamong, which had steeper topography and a higher percentage of peat intersected by streams, soil temperature, the log of stream discharge and the NAO explained 66&thinsp;% of the colour concentrations. Cross-wavelet time-series analysis between river colour and each environmental driver revealed a significant high common power relationship at an annual time step. Each relationship however, varied in phase, further highlighting the complexity of the mechanisms driving river colour in the sub-catchments. The estimated mean annual DOC loads for the Black and Glenamong rivers to Lough Feeagh were similar at 15.0 and 14.7&thinsp;t&thinsp;C&thinsp;km−2&thinsp;yr−1 respectively. The important role of past and current precipitation and, in particular, temperature emphasises the vulnerability of blanket peatland carbon stores to projected climate change and highlights the interaction of local and regional climate in controlling aquatic carbon export. Our results show that water colour (and hence DOC) concentrations can vary considerably between neighbouring catchments and also that regional-scale climatic drivers control the trends in intra- and inter-annual flux of DOC through the system. The combination of locally determined concentrations and regionally controlled fluxes produces aquatic DOC loads that vary over both the annual cycle and over multiple years.</p

    Wintering grounds, population size and evolutionary history of a cryptic passerine species from isotopic and genetic data

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    Cryptic species pose a particular challenge to biologists in the context of life history investigations because of the difficulty in their field discrimination. Additionally, there is normally a lag in their widespread acceptance by the scientific community once they are formally recognised. These two factors might constrain our ability to properly assess the conservation status of the different species conforming a cryptic complex. In this study, we analysed isotopic and genetic data to shed light into the still unclear wintering grounds, population size and evolutionary history of the Iberian chiffchaff Phylloscopus ibericus, a species included within the common chiffchaff Phylloscopus collybita until two decades ago due to their phenotypic similarity. We used molecular methods to identify spring-migrating Phylloscopus species captured in northern Iberia, and by comparing the Hydrogen isotopic ratios of their claw tips (δ2Hc; which would reflect the signatures of their wintering grounds), we detected that δ2Hc values of Iberian chiffchaffs were similar to willow warblers (Phylloscopus trochilus; a renowned trans-Saharan migrant), and higher than common chiffchaffs (mostly a pre-Saharan migrant). These results strongly support the idea that Iberian chiffchaffs winter in tropical Africa. We additionally reconstructed the phylogeny and evolutionary history of the Iberian chiffchaff's clade using mitochondrial and nuclear markers. Our results revealed relatively high values of nucleotide diversity (and, hence, high Ne) for the species that were greater than the values of the common/Iberian most recent common ancestor. This suggests that the Iberian chiffchaff did not experience strong bottlenecks after diverging from the common chiffchaff approximately one million years ago. Ultimately, our study provides another illustrative example of how isotopic and genetic analysis tools can help to enhance our understanding of avian ecology and evolution.Depto. de Biodiversidad, Ecología y EvoluciónFac. de Ciencias BiológicasTRUEpu
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