Analyses of rare collection samples as conservation tool for the last known Italian population of Graphoderus bilineatus (Insecta: Coleoptera)

Graphoderus bilineatus is a predacious diving beetle, widely distributed across Europe. Its poor dispersal ability and the fragmentation and deterioration of its habitats have been indicated as the major causes of decline. In several western European countries, the species is extinct, justifying its inclusion as "vulnerable" in the IUCN red list. Aiming for the conservation of the last known population of G. bilineatus in the northern Italian region of Emilia Romagna, at the lake Pratignano, we surveyed its genetic diversity at the mitochondrial COI gene and compared it to that of other European populations. Two fixed COI haplotypes were found in the Italian and Austrian populations, respectively. Both haplotypes were unique among the European populations surveyed, suggesting these populations suffered a bottleneck and geographic isolation. Populations in western Europe showed lower genetic diversity and higher degree of differentiation than eastern populations. The uniqueness of Pratignano haplotype makes it difficult to choose a source population from which to transfer animals for a possible restocking. Selection of the source population should be based mainly on ecological considerations, but at the same time ensuring a good genetic diversity to maximize the adaptive potential

Acute diarrhea in dogs: Current management and potential role of dietary polyphenols supplementation

Acute diarrhea is one of the most common reasons why pet owners seek veterinary care for their canine companions. In many cases, signs resolve spontaneously or with symptomatic therapy without a specific cause being discovered. However, life-threatening cases can occur. The etiology is complex, including infectious diseases (endoparasites, virus, bacteria, protozoa, fungal agents) by both zoonotic and non-zoonotic pathogens, dietary indiscretion, endocrine diseases, and stress (e.g., travel or environmental changes). In the last years, the role played by oxidative stress in the pathogenesis of acute and chronic enteropathies, independently from the initial noxa, has been highlighted by many researches in both humans and animals. As a result, a series of dietary antioxidant compounds have been studied for their potential use in the treatment of intestinal inflammation. This review summarizes the traditional therapeutic and nutritional options to manage canine acute diarrhea, highlighting the need to explore the role of oxidative stress and potential antioxidant supplementation, especially polyphenols, during acute diarrheic episodes

Faecal microbiota in dogs with multicentric lymphoma

Malignant lymphoma B-cell type is the most common canine haematopoietic malignancy. Changes in intestinal microbiota have been implicated in few types of cancer in humans. The aim of this prospective and case-control study was to determine differences in faecal microbiota between healthy control dogs and dogs with multicentric lymphoma. Twelve dogs affected by multicentric, B-cell, stage III-IV lymphoma, and 21 healthy dogs were enrolled in the study. For each dog, faecal samples were analysed by Illumina sequencing of 16S rRNA genes and quantitative PCR (qPCR) for selected bacterial groups. Alpha diversity was significant lower in lymphoma dogs. Principal coordinate analysis plots showed different microbial clustering (P = .001) and linear discriminant analysis effect size revealed 28 differentially abundant bacterial groups in lymphoma and control dogs. The qPCR analysis showed significant lower abundance of Faecalibacterium spp. (q < .001), Fusobacterium spp. (q = .032), and Turicibacter spp. (q = .043) in dogs with lymphoma compared with control dogs. On the contrary, Streptococcus spp. was significantly higher in dogs with lymphoma (q = .041). The dysbiosis index was significantly higher (P < .0001) in dogs with lymphoma. In conclusion, both sequencing and qPCR analyses provided a global overview of faecal microbial communities and showed significant differences in the microbial communities of dogs presenting with multicentric lymphoma compared with healthy control dogs.dog

Ruthenium(II) Arene Complexes Bearing Tris(pyrazolyl)methanesulfonate Capping Ligands.Electrochemistry, Spectroscopic, and X-ray Structural Characterization

Novel [Ru(L)(Tpms)]Cl and [Ru(L)(Tpms(Ph))]Cl complexes (L = p-cymene, benzene, or hexamethylbenzene, Tpms = tris(pyrazolyl)-methanesulfonate, Tpms(Ph) = tris(3-phenylpyrazoly)methanesulfonate) have been prepared by reaction of [Ru(L)(mu-Cl)(2)](2) with Li[Tpms] and Li[Tpms(Ph)], respectively. [Ru(p-cymene)(Tpms)]BF4 has been synthesized through a metathetic reaction of [Ru(p-cymene)(Tpms)]Cl with AgBF4. [RuCl(cod)(Tpms)] (cod = 1,5-cyclooctadiene) and [RuCl(cod)(Tpms(Ph))] are also reported, being obtained by reaction of [RuCl2(cod)(MeCN)(2)] with Li[Tpms] and Li[Tpms(Ph)], respectively. The structures of the complexes and the coordination modes of the ligands have been established by IR, NMR, and single-crystal X-ray diffraction (for [RuL(Tpms)]X (L = p-cymene or HMB, X = Cl; L = p-cymene, X = BF4)) studies. Electrochemical studies showed that each complex undergoes a single-electron R-II -> R-III oxidation at a potential measured by cyclic voltammetry, allowing to compare the electron-donor characters of the tris(pyrazolyl)methanesulfonate and arene ligands, and to estimate, for the first time, the values of the Lever E-L ligand parameter for Tmps(Ph), HMB, and cod

Escenarios deportivos: Polideportivo los Ángeles

En el presente trabajo planteamos reflexionar sobre un proyecto de renovación y ampliación del área pública con un fuerte énfasis de inclusión social mediante la Arquitectura Deportiva como respuesta a una necesidad territorial y barrial. Se indagó en la lectura del territorio reconociendo sus problemáticas y potencialidades, sus dimensiones culturales y naturales que sirven de base como fundamento para re-descubrir un modo de operar en el lugar que le devuelva el sentido de identidad cultural. El sitio de desarrollo de la propuesta esta ubicado en el Barrio Los Ángeles, próximo al Arroyo Saladillo al sur de la Ciudad de Rosario. El mismo se encuentra en una situación de aislamiento en relación a la ciudad con vulnerabilidad social y espacial, en un área muy degradada, con una topografía inundable como vestigio del paso del antiguo cauce del arroyo y con necesidades básicas insatisfechas. Esto se ve reflejado en el tejido urbano, en la división social, en la actividad económica, en la lejanía de instituciones educativas para los jóvenes, en las carencias de infraestructuras y en la falta de equipamientos de interés social. Frente a estas dificultades se pretende que el proyecto permita la puesta en valor del sector proporcionando espacios recreativos articulados con las preexistencias como íconos de identidad cultural del lugar cuya finalidad es una mayor interacción y apropiación por parte de la comunidad en general y del barrio en particular. El edificio plantea la creación de espacios deportivos amalgamando la idea de un club de barrio a una escala mayor, dotando a la periferia de infraestructura, siendo un punto estratégico de vinculación con la ciudad y el área metropolitana. La exploración proyectual se basó en un estudio exhaustivo de casos como herramienta para la formación y desarrollo de un edificio que contemple varias actividades deportivas en un gran espacio cubierto, incluyendo sus instalaciones de manera que las mismas funcionen independientes. Las intenciones proyectuales buscan abrirse hacia las visuales del arroyo, generando una zona de juego exterior de contacto con la naturaleza como principal protagonista del espacio. El edificio se adapta a su entorno, respetando las diferentes escalas, ya sea la barrial y humana en contraposición con la imponente de la naturaleza. Comprende su territorio y busca jugar y adaptarse a la topografía del mismo, dejando un basamento de hormigón como elemento pesado que decrece hacia la liviandad de una estructura metálica reafirmando la intención de desprendimiento de los diferentes elementos y materiales. Nuestra expectativa con este trabajo es el repensar el desarrollo de actividades recreativas públicas en áreas de riesgo socio-ambiental, para contribuir al desarrollo territorial con equidad, siendo en este caso de estudio la práctica deportiva una herramienta indispensable de inclusión social.Fil: Cerquetella, Ornella. Universidad Nacional de Rosario. Facultad de Arquitectura, Planeamiento y Diseño; ArgentinaFil: Roncaglia, M. Belén. Universidad Nacional de Rosario. Facultad de Arquitectura, Planeamiento y Diseño; Argentin

Analyses of rare collection samples as conservation tool for the last known Italian population of Graphoderus bilineatus (Insecta: Coleoptera)

Graphoderus bilineatus is a predacious diving beetle, widely distributed across Europe. Its poor dispersal ability and the fragmentation and deterioration of its habitats have been indicated as the major causes of decline. In several western European countries, the species is extinct, justifying its inclusion as “vulnerable” in the IUCN red list. Aiming for the conservation of the last known population of G. bilineatus in the northern Italian region of Emilia Romagna, at the lake Pratignano, we surveyed its genetic diversity at the mitochondrial COI gene and compared it to that of other European populations. Two fixed COI haplotypes were found in the Italian and Austrian populations, respectively. Both haplotypes were unique among the European populations surveyed, suggesting these populations suffered a bottleneck and geographic isolation. Populations in western Europe showed lower genetic diversity and higher degree of differentiation than eastern populations. The uniqueness of Pratignano haplotype makes it difficult to choose a source population from which to transfer animals for a possible restocking. Selection of the source population should be based mainly on ecological considerations, but at the same time ensuring a good genetic diversity to maximize the adaptive potential