30 research outputs found
A cartilage tissue engineering approach combining starch-polycaprolactone fibre mesh scaffolds with bovine articular chondrocytes
In the present work we originally tested the suitability
of corn starch-polycaprolactone (SPCL) scaffolds for
pursuing a cartilage tissue engineering approach. Bovine articular
chondrocytes were seeded on SPCL scaffolds under
dynamic conditions using spinner flasks (total of 4 scaffolds
per spinner flask using cell suspensions of 0.5×106 cells/ml)
and cultured under orbital agitation for a total of 6 weeks.
Poly(glycolic acid) (PGA) non-woven scaffolds and bovine
native articular cartilage were used as standard controls for
the conducted experiments. PGA is a kind of standard in
tissue engineering approaches and it was used as a control
in that sense. The tissue engineered constructs were characterized
at different time periods by scanning electron microscopy
(SEM), hematoxylin-eosin (H&E) and toluidine
blue stainings, immunolocalisation of collagen types I and II,
and dimethylmethylene blue (DMB) assay for glycosaminoglycans
(GAG) quantification assay. SEM results for SPCL
constructs showed that the chondrocytes presented normal
morphological features, with extensive cells presence at the
surface of the support structures, and penetrating the scaffolds
pores. These observations were further corroborated
by H&E staining. Toluidine blue and immunohistochemistry
exhibited extracellular matrix deposition throughout the 3D structure. Glycosaminoglycans, and collagen types I and II
were detected. However, stronger staining for collagen type
II was observed when compared to collagen type I. The PGA
constructs presented similar features toSPCLat the end of the
6 weeks. PGA constructs exhibited higher amounts of matrix
glycosaminoglycans when compared to the SPCL scaffolds.
However, we also observed a lack of tissue in the central
area of the PGA scaffolds. Reasons for these occurrences
may include inefficient cells penetration, necrosis due to high
cell densities, or necrosis related with acidic by-products
degradation. Such situation was not detected in the SPCL
scaffolds, indicating the much better biocompatibility of the
starch based scaffolds
Interaction between Axons and Specific Populations of Surrounding Cells Is Indispensable for Collateral Formation in the Mammillary System
An essential phenomenon during brain development is the extension of long collateral branches by axons. How the local cellular environment contributes to the initial sprouting of these branches in specific points of an axonal shaft remains unclear.The principal mammillary tract (pm) is a landmark axonal bundle connecting ventral diencephalon to brainstem (through the mammillotegmental tract, mtg). Late in development, the axons of the principal mammillary tract sprout collateral branches at a very specific point forming a large bundle whose target is the thalamus. Inspection of this model showed a number of distinct, identified cell populations originated in the dorsal and the ventral diencephalon and migrating during development to arrange themselves into several discrete groups around the branching point. Further analysis of this system in several mouse lines carrying mutant alleles of genes expressed in defined subpopulations (including Pax6, Foxb1, Lrp6 and Gbx2) together with the use of an unambiguous genetic marker of mammillary axons revealed: 1) a specific group of Pax6-expressing cells in close apposition with the prospective branching point is indispensable to elicit axonal branching in this system; and 2) cooperation of transcription factors Foxb1 and Pax6 to differentially regulate navigation and fasciculation of distinct branches of the principal mammillary tract.Our results define for the first time a model system where interaction of the axonal shaft with a specific group of surrounding cells is essential to promote branching. Additionally, we provide insight on the cooperative transcriptional regulation necessary to promote and organize an intricate axonal tree
High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients
Approximately 25% of all patients with stage II colorectal cancer will experience recurrent disease and subsequently die within 5 years. MicroRNA-21 (miR-21) is upregulated in several cancer types and has been associated with survival in colon cancer. In the present study we developed a robust in situ hybridization assay using high-affinity Locked Nucleic Acid (LNA) probes that specifically detect miR-21 in formalin-fixed paraffin embedded (FFPE) tissue samples. The expression of miR-21 was analyzed by in situ hybridization on 130 stage II colon and 67 stage II rectal cancer specimens. The miR-21 signal was revealed as a blue chromogenic reaction, predominantly observed in fibroblast-like cells located in the stromal compartment of the tumors. The expression levels were measured using image analysis. The miR-21 signal was determined as the total blue area (TB), or the area fraction relative to the nuclear density (TBR) obtained using a red nuclear stain. High TBR (and TB) estimates of miR-21 expression correlated significantly with shorter disease-free survival (p = 0.004, HR = 1.28, 95% CI: 1.06–1.55) in the stage II colon cancer patient group, whereas no significant correlation with disease-free survival was observed in the stage II rectal cancer group. In multivariate analysis both TB and TBR estimates were independent of other clinical parameters (age, gender, total leukocyte count, K-RAS mutational status and MSI). We conclude that miR-21 is primarily a stromal microRNA, which when measured by image analysis identifies a subgroup of stage II colon cancer patients with short disease-free survival
Enhanced anxiety and stress-induced corticosterone release are associated with increased Crh expression in a mouse model of Rett syndrome
Rett syndrome (RTT), a postnatal neurodevelopmental disorder, is caused by mutations in the methyl-CpG-binding protein 2 (MECP2) gene. Children with RTT display cognitive and motor abnormalities as well as autistic features. We studied mice bearing a truncated Mecp2 allele (Mecp2(308/Y) mice) and found evidence of increased anxiety-like behavior and an abnormal stress response as evidenced by elevated serum corticosterone levels. We found increased corticotropin-releasing hormone (Crh) gene expression in the paraventricular nucleus of the hypothalamus, the central amygdala, and the bed nucleus of the stria terminalis. Finally, we discovered that MeCP2 binds the Crh promoter, which is enriched for methylated CpG dinucleotides. In contrast, the MeCP2(308) protein was not detected at the Crh promoter. This study identifies Crh as a target of MeCP2 and implicates Crh overexpression in the development of specific features of the Mecp2(308/Y) mouse, thereby providing opportunities for clinical investigation and therapeutic intervention in RTT
Human chromosome 21 gene expression atlas in the mouse
Genome-wide expression analyses have a crucial role in functional genomics. High resolution methods, such as RNA in situ hybridization provide an accurate description of the spatiotemporal distribution of transcripts as well as a three-dimensional 'in vivo' gene expression overview. We set out to analyse systematically the expression patterns of genes from an entire chromosome. We chose human chromosome 21 because of the medical relevance of trisomy 21 (Down's syndrome). Here we show the expression analysis of all identifiable murine orthologues of human chromosome 21 genes (161 out of 178 confirmed human genes) by RNA in situ hybridization on whole mounts and tissue sections, and by polymerase chain reaction with reverse transcription on adult tissues. We observed patterned expression in several tissues including those affected in trisomy 21 phenotypes (that is, central nervous system, heart, gastrointestinal tract, and limbs). Furthermore, statistical analysis suggests the presence of some regions of the chromosome with genes showing either lack of expression or, to a lesser extent, co-expression in specific tissues. This high resolution expression 'atlas' of an entire human chromosome is an important step towards the understanding of gene function and of the pathogenetic mechanisms in Down's syndrome
Human chromosome 21 gene expression atlas in the mouse.
The study of gene expression patterns is of crucial importance to the understanding of gene function. We set out to systematically analyze the expression patterns of genes from an entire chromosome. We chose human chromosome 21 (HC21) because its entire finished nucleotide sequence was available. Furthermore trisomy 21 (Down syndrome, DS) is the most common genetic cause of mental retardation. This chromosome-based approach has advantages over the study of an arbitrary set of genes, as it provides information on the consequences of aneuploidy, and may allow the identification of clusters of co-regulated genes. All identifiable murine orthologs of human chromosome 21 genes (161 out of 178 confirmed human genes) were analyzed by RNA in situ hybridization on whole-mounts (E9.5, E10.5) and tissue sections (E14.5), and by RT-PCR on adult tissues. These stages correspond to mid and late embryonic and fetal human periods, when the major organs and body regions are organized. Patterned expression was observed in several tissues including those affected in trisomy 21 phenotypes (i.e. CNS, heart, gastrointestinal tract, and limbs). Furthermore, statistical analysis suggests the presence of clusters of genes with significant patterns of either coexpression or lack of expression in specific tissues. The entire data set was incorporated in a web site that will be made available to the scientific community. This high resolution expression "atlas" of an entire human chromosome is a new level of gene annotation, which is likely to advance our knowledge on gene function and regulation and to the understanding of human aneuploidies, such as DS
Human chromosome 21 gene expression atlas in the mouse.
Genome-wide expression analyses have a crucial role in functional genomics. High resolution methods, such as RNA in situ hybridization provide an accurate description of the spatiotemporal distribution of transcripts as well as a three-dimensional 'in vivo' gene expression overview. We set out to analyse systematically the expression patterns of genes from an entire chromosome. We chose human chromosome 21 because of the medical relevance of trisomy 21 (Down's syndrome). Here we show the expression analysis of all identifiable murine orthologues of human chromosome 21 genes (161 out of 178 confirmed human genes) by RNA in situ hybridization on whole mounts and tissue sections, and by polymerase chain reaction with reverse transcription on adult tissues. We observed patterned expression in several tissues including those affected in trisomy 21 phenotypes (that is, central nervous system, heart, gastrointestinal tract, and limbs). Furthermore, statistical analysis suggests the presence of some regions of the chromosome with genes showing either lack of expression or, to a lesser extent, co-expression in specific tissues. This high resolution expression 'atlas' of an entire human chromosome is an important step towards the understanding of gene function and of the pathogenetic mechanisms in Down's syndrome