A versatile all-channel stimulator for electrode arrays, with real-time control

Over the last few decades, technology to record through ever increasing numbers of electrodes has become available to electrophysiologists. For the study of distributed neural processing, however, the ability to stimulate through equal numbers of electrodes, and thus to attain bidirectional communication, is of paramount importance. Here, we present a stimulation system for multi-electrode arrays which interfaces with existing commercial recording hardware, and allows stimulation through any electrode in the array, with rapid switching between channels. The system is controlled through real-time Linux, making it extremely flexible: stimulation sequences can be constructed on-the-fly, and arbitrary stimulus waveforms can be used if desired. A key feature of this design is that it can be readily and inexpensively reproduced in other labs, since it interfaces to standard PC parallel ports and uses only off-the-shelf components. Moreover, adaptation for use with in vivo multi-electrode probes would be straightforward. In combination with our freely available data-acquisition software, MeaBench, this system can provide feedback stimulation in response to recorded action potentials within 15 ms

Persistent dynamic attractors in activity patterns of cultured neuronal networks

Three remarkable features of the nervous system—complex spatiotemporal patterns, oscillations, and persistent activity—are fundamental to such diverse functions as stereotypical motor behavior, working memory, and awareness. Here we report that cultured cortical networks spontaneously generate a hierarchical structure of periodic activity with a strongly stereotyped population-wide spatiotemporal structure demonstrating all three fundamental properties in a recurring pattern. During these "superbursts," the firing sequence of the culture periodically converges to a dynamic attractor orbit. Precursors of oscillations and persistent activity have previously been reported as intrinsic properties of the neurons. However, complex spatiotemporal patterns that are coordinated in a large population of neurons and persist over several hours—and thus are capable of representing and preserving information—cannot be explained by known oscillatory properties of isolated neurons. Instead, the complexity of the observed spatiotemporal patterns implies large-scale self-organization of neurons interacting in a precise temporal order even in vitro, in cultures usually considered to have random connectivity

A two-dimensional mixing length theory of convective transport

The helioseismic observations of the internal rotation profile of the Sun raise questions about the two-dimensional (2D) nature of the transport of angular momentum in stars. Here we derive a convective prescription for axisymmetric (2D) stellar evolution models. We describe the small scale motions by a spectrum of unstable linear modes in a Boussinesq fluid. Our saturation prescription makes use of the angular dependence of the linear dispersion relation to estimate the anisotropy of convective velocities. We are then able to provide closed form expressions for the thermal and angular momentum fluxes with only one free parameter, the mixing length. We illustrate our prescription for slow rotation, to first order in the rotation rate. In this limit, the thermodynamical variables are spherically symetric, while the angular momentum depends both on radius and latitude. We obtain a closed set of equations for stellar evolution, with a self-consistent description for the transport of angular momentum in convective regions. We derive the linear coefficients which link the angular momentum flux to the rotation rate ($\Lambda$- effect) and its gradient ($\alpha$-effect). We compare our results to former relevant numerical work.Comment: MNRAS accepted, 10 pages, 1 figure, version prior to language editio

The Mystical Paradigm in Shakespeare’s King Lear

This article takes as its starting point the statement by Caroline Spurgeon that mysticism, with its aim of the union with the One, does not appear in Shakespeare's works. It is initially pointed out that any suggestion that mysticism is present in any work is fraught with serious reception problems, since mysticism is very adversely viewed at present, even by supposedly open-minded literary critics. After providing a definition of mysticism in terms which attempt to alleviate these reception problems, evidence will then be presented from King Lear to suggest that the play is built on the basis of an affirmation of unity, and a perception of disunity as resulting from the destructive effects of difference, division and duality. This, it is suggested, implies that Shakespeare was thinking in terms of the mystical paradigm when he wrote the play. In conclusion, some indication is given of possible significances of the presence of the mystical paradigm in the play, and how richly suggestive the presence of such a paradigm is in a play such as this

Femtosecond probing of bimolecular reactions: The collision complex

Progress has been made in probing the femtosecond dynamics of transition states of chemical reactions.(1) The "half-collision" case of unimolecular reactions has been experimentally investigated for a number of systems and much theoretical work has already been developed.(2) For bimolecular reactions, the case of full collision, the zero of time is a problem which makes the femtosecond temporal resolution of the dynamics a difficult task

Femtosecond real-time probing of reactions. VIII. The bimolecular reaction Br+I2

In this paper, we discuss the experimental technique for real-time measurement of the lifetimes of the collision complex of bimolecular reactions. An application to the atom–molecule Br+I_2 reaction at two collision energies is made. Building on our earlier Communication [J. Chem. Phys. 95, 7763 (1991)], we report on the observed transients and lifetimes for the collision complex, the nature of the transition state, and the dynamics near threshold. Classical trajectory calculations provide a framework for deriving the global nature of the reactive potential energy surface, and for discussing the real-time, scattering, and asymptotic (product-state distribution) aspects of the dynamics. These experimental and theoretical results are compared with the extensive array of kinetic, crossed beam, and theoretical studies found in the literature for halogen radical–halogen molecule exchange reactions

A study of the relative effectiveness and cost of computerized information retrieval in the interactive mode

Results of a number of experiments to illuminate the relative effectiveness and costs of computerized information retrieval in the interactive mode are reported. It was found that for equal time spent in preparing the search strategy, the batch and interactive modes gave approximately equal recall and relevance. The interactive mode however encourages the searcher to devote more time to the task and therefore usually yields improved output. Engineering costs as a result are higher in this mode. Estimates of associated hardware costs also indicate that operation in this mode is more expensive. Skilled RECON users like the rapid feedback and additional features offered by this mode if they are not constrained by considerations of cost

Early conceptual and linguistic processes operate in independent channels

Language and concepts are intimately linked, but how do they interact? In the study reported here, we probed the relation between conceptual and linguistic processing at the earliest processing stages. We presented observers with sequences of visual scenes lasting 200 or 250 ms per picture. Results showed that observers understood and remembered the scenes’ abstract gist and, therefore, their conceptual meaning. However, observers remembered the scenes at least as well when they simultaneously performed a linguistic secondary task (i.e., reading and retaining sentences); in contrast, a nonlinguistic secondary task (equated for difficulty with the linguistic task) impaired scene recognition. Further, encoding scenes interfered with performance on the nonlinguistic task and vice versa, but scene processing and performing the linguistic task did not affect each other. At the earliest stages of conceptual processing, the extraction of meaning from visually presented linguistic stimuli and the extraction of conceptual information from the world take place in remarkably independent channels

Large capacity temporary visual memory

Visual working memory (WM) capacity is thought to be limited to three or four items. However, many cognitive activities seem to require larger temporary memory stores. Here, we provide evidence for a temporary memory store with much larger capacity than past WM capacity estimates. Further, based on previous WM research, we show that a single factor|proactive interference|is su cient to bring capacity estimates down to the range of previousWM capacity estimates. Participants saw a rapid serial visual presentation (RSVP) of 5 to 21 pictures of familiar objects or words presented at rates of 4/s or 8/s, respectively, and thus too fast for strategies such as rehearsal. Recognition memory was tested with a single probe item. When new items were used on all trials, no xed memory capacities were observed, with estimates of up to 9.1 retained pictures for 21-item lists, and up to 30.0 retained pictures for 100-item lists, and no clear upper bound to how many items could be retained. Further, memory items were not stored in a temporally stable form of memory, but decayed almost completely after a few minutes. In contrast, when, as in most WM experiments, a small set of items was reused across all trials, thus creating proactive interference among items, capacity remained in the range reported in previous WM experiments. These results show that humans have a large-capacity temporary memory store in the absence of proactive interference, and raise the question of whether temporary memory in everyday cognitive processing is severely limited as in WM experiments, or has the much larger capacity found in the present experiments

Simultaneous optical polarimetry and X-ray data of the near synchronous polar RX J2115-5840

We present simultaneous optical polarimetry and X-ray data of the near synchronous polar RX J2115-5840. We model the polarisation data using the Stokes imaging technique of Potter et al. We find that the data are best modelled using a relatively high binary inclination and a small angle between the magnetic and spin axes. We find that for all spin-orbit beat phases, a significant proportion of the accretion flow is directed onto the lower hemisphere of the white dwarf, producing negative circular polarisation. Only for a small fraction of the beat cycle is a proportion of the flow directed onto the upper hemisphere. However, the accretion flow never occurs near the upper magnetic pole, whatever the orientation of the magnetic poles. This indicates the presence of a non-dipole field with the field strength at the upper pole significantly higher. We find that the brightest parts of the hard X-ray emitting region and the cyclotron region are closely coincident.Comment: 9 pages, accepted for publication in MNRAS 2 March 200