75 research outputs found
In situ measurements of density fluctuations and compressibility in silica glass as a function of temperature and thermal history
In this paper, small-angle X-ray scattering measurements are used to
determine the different compressibility contributions, as well as the
isothermal compressibility, in thermal equilibrium in silica glasses having
different thermal histories. Using two different methods of analysis, in the
supercooled liquid and in the glassy state, we obtain respectively the
temperature and fictive temperature dependences of the isotheraml
compressibility. The values obtained in the glass and supercooled liquid states
are very close to each other. They agree with previous determinations of the
literature. The compressibility in the glass state slightly decreases with
increasing fictive temperature. The relaxational part of the compressibility is
also calculated and compared to previous determinations. We discussed the small
differences between the different determinations
Quantum statistical effects in nano-oscillator arrays
We have theoretically predicted the density of states(DOS), the low
temperature specific heat, and Brillouin scattering spectra of a large, free
standing array of coupled nano-oscillators. We have found significant gaps in
the DOS of 2D elastic systems, and predict the average DOS to be nearly
independent of frequency over a broad band f < 50GHz. At low temperatures, the
measurements probe the quantum statistics obeyed by rigid body modes of the
array and, thus, could be used to verify the quantization of the associated
energy levels. These states, in turn, involve center-of mass motion of large
numbers of atoms, N > 1.e14, and therefore such observations would extend the
domain in which quantum mechanics has been experimentally tested. We have found
the required measurement capability to carry out this investigation to be
within reach of current technology.Comment: 1 tex file, 3 figures, 1 bbl fil
Voltage-programmable liquid optical interface
Recently, there has been intense interest in photonic devices based on microfluidics, including displays and refractive tunable microlenses and optical beamsteerers, that work using the principle of electrowetting. Here, we report a novel approach to optical devices in which static wrinkles are produced at the surface of a thin film of oil as a result of dielectrophoretic forces. We have demonstrated this voltage-programmable surface wrinkling effect in periodic devices with pitch lengths of between 20 and 240 µm and with response times of less than 40 µs. By a careful choice of oils, it is possible to optimize either for high-amplitude sinusoidal wrinkles at micrometre-scale pitches or more complex non-sinusoidal profiles with higher Fourier components at longer pitches. This opens up the possibility of developing rapidly responsive voltage-programmable, polarization-insensitive transmission and reflection diffraction devices and arbitrary surface profile optical devices
Water Dynamics at Protein Interfaces: Ultrafast Optical Kerr Effect Study
The behavior of water molecules surrounding a protein can have an important bearing on its structure and function. Consequently, a great deal of attention has been focused on changes in the relaxation dynamics of water when it is located at the protein surface. Here we use the ultrafast optical Kerr effect to study the H-bond structure and dynamics of aqueous solutions of proteins. Measurements are made for three proteins as a function of concentration. We find that the water dynamics in the first solvation layer of the proteins are slowed by up to a factor of 8 in comparison to those in bulk water. The most marked slowdown was observed for the most hydrophilic protein studied, bovine serum albumin, whereas the most hydrophobic protein, trypsin, had a slightly smaller effect. The terahertz Raman spectra of these protein solutions resemble those of pure water up to 5 wt % of protein, above which a new feature appears at 80 cm–1, which is assigned to a bending of the protein amide chain
The effect of simulated microgravity on osteoblasts is independent of the induction of apoptosis
Bone loss during spaceflight has been attributed, in part, to a reduction in osteoblast number, altered gene expression, and an increase in cell death. To test the hypothesis that microgravity induces osteoblast apoptosis and suppresses the mature phenotype, we created a novel system to simulate spaceflight microgravity combining control and experimental cells within the same in vitro environment. Cells were encapsulated into two types of alginate carriers: non-rotationally stabilized (simulated microgravity) and rotationally stabilized (normal gravity). Using these specialized carriers, we were able to culture MC3T3-E1 osteoblast-like cells for 1-14 days in simulated microgravity and normal gravity in the same rotating wall vessel (RWV). The viability of cells was not affected by simulated microgravity, nor was the reductive reserve. To determine if simulated microgravity sensitized the osteoblasts to apoptogens, cells were challenged with staurosporine or sodium nitroprusside and the cell death was measured. Simulated microgravity did not alter the sensitivity of C3H10T-1/2 stem cells, MC3T3-E1 osteoblast-like cells, or MLO-A5 osteocyte-like cells to the action of these agents. RT-PCR analysis indicated that MC3T3-E1 osteoblasts maintained expression of RUNX2, osteocalcin, and collagen type I, but alkaline phosphatase expression was decreased in cells subjected to simulated microgravity for 5 days. We conclude that osteoblast apoptosis is not induced by vector-averaged gravity, thus suggesting that microgravity does not directly induce osteoblast death. © 2007 Wiley-Liss, Inc
Bone cell survival in microgravity: Evidence that modeled microgravity increases osteoblast sensitivity to apoptogens
Studies were performed to evaluate the effects of modeled microgravity on the induction of osteoblast apoptosis. MC3T3-E1 osteoblast-like cells were cultured in alginate carriers in the NASA-approved high aspect ratio vessel (HARV). This system subjects the cells to a time-averaged gravitational field (vector-averaged gravity) to simulate low gravity conditions. Cells were cultured in the HARV for five days, and then examined for apoptosis. In simulated microgravity, the cells remained vital, although analysis of expressed genes indicated that there was loss of the mature osteoblast phenotype. Additionally, we noted that there was a loss of the mitochondrial membrane potential, a low level of the antiapoptotic protein Bcl-2, as well as Akt protein, and the redox status of the cells was disturbed. All of these parameters indicated that vector-averaged gravity disrupts mitochondrial function, thereby sensitizing osteoblasts to apoptosis. We then used a challenge assay to evaluate the apoptotic sensitivity of the cells subjected to vector-averaged gravity. When challenged with staurosporine, cells subjected to vector-averaged gravity evidenced elevated levels of cell death relative to control cell populations. Another objective of the study was to improve upon conventional carriers by using alginate encapsulation to support cells in the HARV. We have demonstrated that the alginate carrier system affords a more robust system than surface-seeded carriers. This new system has the advantage of shielding cells from mechanical damage and fluid shear stresses on cells in the HARV, permitting carefully controlled studies of the effects of vector-averaged gravity
Numerical modeling of oxygen distributions in cortical and cancellous bone: oxygen availability governs osteonal and trabecular dimensions
Whereas recent work has demonstrated the role of oxygen tension in the regulation of skeletal cell function and viability, the microenvironmental oxemic status of bone cells remains unknown. In this study, we have employed the Krogh cylinder model of oxygen diffusion to predict the oxygen distribution profiles in cortical and cancellous bone. Under the assumption of saturation-type Michaelis-Menten kinetics, our numerical modeling has indicated that, under steady-state conditions, there would be oxygen gradients across mature osteons and trabeculae. In Haversian bone, the calculated oxygen tension decrement ranges from 15 to 60%. For trabecular bone, a much shallower gradient is predicted. We note that, in Haversian bone, the gradient is largely dependent on osteocyte oxygen utilization and tissue oxygen diffusivity; in trabecular bone, the gradient is dependent on oxygen utilization by cells lining the bone surface. The Krogh model also predicts dramatic differences in oxygen availability during bone development. Thus, during osteon formation, the modeling equations predict a steep oxygen gradient at the initial stage of development, with the gradient becoming lesser as osteonal layers are added. In contrast, during trabeculum formation, the oxygen gradient is steepest when the diameter of the trabeculum is maximal. Based on these results, it is concluded that significant oxygen gradients exist within cortical and cancellous bone and that the oxygen tension may regulate the physical dimensions of both osteons and bone trabeculae
Induction of osteopenia during experimental tooth movement in the rat: Alveolar bone remodelling and the mechanostat theory
10.1093/ejo/cjp032European Journal of Orthodontics313221-231EJOO
High Throughput, High Resolution Enzymatic Lithography Process: Effect of Crystallite Size, Moisture, and Enzyme Concentration
By bringing enzymes into contact
with predefined regions of a surface,
a polymer film can be selectively degraded to form desired patterns
that find a variety of applications in biotechnology and electronics.
This so-called “enzymatic lithography” is an environmentally
friendly process as it does not require actinic radiation or synthetic
chemicals to develop the patterns. A significant challenge to using
enzymatic lithography has been the need to restrict the mobility of
the enzyme in order to maintain control of feature sizes. Previous
approaches have resulted in low throughput and were limited to polymer
films only a few nanometers thick. In this paper, we demonstrate an
enzymatic lithography system based on Candida antartica lipase B (CALB) and poly(ε-caprolactone) (PCL) that can resolve
fine-scale features, (<1 μm across) in thick (0.1–2.0
μm) polymer films. A
Polymer Pen Lithography (PPL) tool was developed to deposit an aqueous
solution of CALB onto a spin-cast PCL film. Immobilization of the
enzyme on the polymer surface was monitored using fluorescence microscopy
by labeling CALB with FITC. The crystallite size in the PCL films
was systematically varied; small crystallites resulted in significantly
faster etch rates (20 nm/min) and the ability to resolve smaller features
(as fine as 1 μm). The effect of printing conditions and relative
humidity during incubation is also presented. Patterns formed in the
PCL film were transferred to an underlying copper foil demonstrating
a “Green” approach to the fabrication of printed circuit
boards
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