Determination of the response of Gypsophila arrostii Guss. to boron under in vitro conditions

In this research, the response of the plant Gypsophila arrostii Guss. to boron (B) under in vitro conditions was examined. The seeds were cultured on MS medium including 0, 10, 20, 40, 80 mg B l-1. Seedlings obtained from germinated seeds and grown in a culture medium for 8 weeks were analyzed. At the end of this period, stem length (cm), root length (cm), plant weight (g) and elemental content (mg kg-1) of the plants were determined. According to the results, the seeds of G. arrostii Guss. could germinate on media with up to 80 mg B l-1, and the seedlings demonstrated an ability to survive, albeit poorly, a dose of boron as high as 80 mg B l-1. In the experiment, the highest stem length (7.5 cm) was obtained from the 20 mg B l-1 treatment and the highest stem fresh weight (0.9 g) and stem dry weight (0.19 g) were measured in the 10 mg B l-1 variant. No significant statistical difference was determined between the boron treatments in terms of root length, root fresh weight and root dry weight. Our evaluation of the elemental content of plants demonstrated that the amount of boron in the root and stem increased parallel to its increase in the growth media. In the 80 mg B l-1 treatment, 601.9 mg kg-1 boron in root and 1,035.4 mg kg-1 boron in stem were determined. Besides, it was discovered that the contents of K, Mg, Zn, Na in root decreased while the contents of P, B, Mn, Cu in root increased in response to the growing amount of boron in the environment. In response to the increasing boron concentrations, the content of K, P, Mn, Cu, Zn and S increased while the amount of Ca, Mg and Na in the plant stem decreased. Consequently, G. arrostii Guss. was found to be a boron hyperaccumulator, collecting boron in tissues (in the roots and stems), in which it resembled some other types of Gypsophilla

Contents of trace elements and sulphur in the leaves of deciduous trees along the roads of Zonguldak, Turkey

In this study, 16 trace elements (Al, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Ni, Pb, Sr, V, Zn) and S were found in unwashed leaves of several deciduous tree species [Alnus glutinosa (L.) Gaertner, Platanus orientalis L., Salix babylonica L.]. Samples were collected from 28 points distributed over 5 sites representing different traffic intensities in the city of Zonguldak. The relationships of sampling sites were determined using the UPGMA method. According to the results of analyses, the trace element and S concentrations in the observed tree species were ordered as follows: S>Mg>Fe>Sr>Zn> B>Ba>Al>Mn>Cu>Pb> Ni> V>Cr>Li>Co>Cd in Alnus glutinosa; S>Mg>Fe>Sr>Zn> Mn> Ba >Al>B>Cu>Pb> Cr>V>Li>Ni>Co>Cd in Platanus orientalis; S>Fe>Mg>Zn> Sr>Al>Ba>B>Mn>Cu> V>Pb>Ni>Li>Cr>Cd>Co in Salix babylonica. Significant positive correlations existed between the amount of heavy metals (r=0.81-1.00). The results are discussed in the context of prior studies. © by PSP

Effects of different types of mycorrhiza on the development and the elemental content of lupin (Lupinus albus L.)

The effects of different types of arbuscular mycorrhizal (AM) fungi on the root inoculation and plant elemental content of lupin (Lupinus albus L.) were investigated in the present study. The growth and development of lupin were examined to determine the species of AM fungi that can help to grow lupin with a high protein content and economic value. In this study, which was carried out as a pot experiment under controlled greenhouse conditions, first the pots were inoculated with Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium mycorrhizal spores and then lupin (Lupinus albus) seeds were sown. The plants were watered with pure water during the experiment. The trial was terminated after a 60-day plant gowing period. In the study, inoculation occurred at lupin roots at rates varying between 13.3 and 30.0%. However, there was no statistically significant difference among the types of arbuscular mycorrhizal fungi applied to the plant in the inoculation rate. The examination of the effect of the application of different AM (Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium) spore on the plant development showed that AM inoculation did not have an effect on the lupin development. The effect of AM inoculation on the plant’s nutrient content revealed no significant difference in the content of crude protein, P and K, while demonstrating a significant increase in the sulphur and magnesium content versus the control. The plant content of crude protein varied between 185.6 and 226.5 (g kg-1), phosphorus – 0.61-0.74 (g kg-1) and potassium – 9.6-11.1 (g kg-1). The concentrations of Zn, Cu, B and Mo in lupin did not show statistically significant modifications caused by the inoculation of different types of AM. However, the plant Mn content showed a decrease due to AM inoculation, whereas a significant increase was observed in the Na content after AM inoculation. AM fungi were observed in plant roots after the inoculation with any of the four different types of mycorrhiza. But no positive effects of mycorrhizal inoculation were not observed on crude protein and the uptake of plant nutrients

The role of food allergy in otitis media with effusion

PubMedID: 15195062Objective To detect the relationship between food allergy and otitis media with effusion (OME). Materials and methods This study was performed on three different groups. The patient group was made up of 56 patients with OME (group I). There were 28 patients with food allergy in group II and these patients were investigated for OME. The control group consisted of 28 patients without any complaints concerning food allergy or OME (group III). Results Food allergy was detected in 25 patients with OME (44.6%) (group I). In patients with food allergy (group II), OME was detected in 7 patients (25%). In the control group (group III) food allergy was diagnosed in 5 patients (18%) and OME in 1 patient (3%). The incidence of food allergy in OME group was statistically significant when compared to the normal group (P > 0.05). Conclusions This study demonstrates that food allergy may play a role in the etiopathogenesis of OME

Yr10 gene polymorphism in bread wheat varieties

Yellow rust resistance locus Yr10 located on chromosome 1B in Moro and originated from the Turkish line PI178383 was investigated in terms of polymorphism in seven winter type bread wheat cvs.(Triticum aestivum ssp. Aestivum) Altay2000, zgi2001, Sönmez2001 (yellow rust resistant), Aytýn98, ES14, Harmankaya99 (yellow rust susceptible) and PI178383 as control. Exon 1 (1 - 833 bp) and Exon 2 (1989 - 3630 bp) parts of Yr10 were amplified with three primers. Amplification was not observed with E2A primers in Harmankaya99, zgi2001 and Sönmez2001 cvs, while amplification products were observable at all tested varieties with the other primers. PCR results showed that E2A reverse primer is not able to anneal to the three varieties mentioned above. Sequence analysis and bioinformaticsanalysis proved that there has been single nucleotide changes especially in the second exon. The most similar sequences to the first exon of Harmankaya99, zgi01 and Sönmez2001 are AF509535 (Aegilops tauschii NBS-LRR-like gene), AF509534 (A. tauschii NBS-LRR-like gene sequence) and AF509534, respectively. These results could be helpful in revealing divergence between resistant and susceptible varieties