One-year outcomes of a bilateral randomised prospective clinical trial comparing PRK with mitomycin C and LASIK

Aim: To compare 1-year follow-up results of photorefractive keratectomy (PRK) with mitomycin C (MMC) and laser in situ keratomileusis (LASIK) for custom correction of myopia.Methods: Eighty-eight eyes of 44 patients with moderate myopia were randomised to PRK with 0.002% MMC for 1 min in one eye and LASIK in the fellow eye. the 1-year follow-up was evaluated.Results: There were no differences between LASIK and MMC-PRK eyes preoperatively. Forty-two patients completed the 1-year follow-up. MMC-PRK eyes achieved better uncorrected visual acuity (p=0.03) and better best-spectacle-corrected visual acuity (p<0.001) 1 year after surgery. SE did not differ in the two groups during follow-up (p=0.12). Clinically significant haze was not found in surface ablation eyes. LASIK eyes showed a greater higher-order aberration (p=0.01) and lower contrast sensitivity (p<0.05) than MMC-PRK eyes postoperatively. Excellent vision was reported in 64% of LASIK and 74% of MMC-PRK eyes 1 year after surgery. the corneal resistance factor and corneal hysteresis (ORA, Reichert) were higher in LASIK than in MMC-PRK eyes (p<0.01) at the last follow-up.Conclusions: Wavefront-guided PRK with 0.002% MMC was more effective than wavefront-guided LASIK for correction of moderate myopia. Further research is necessary to determine the optimal concentration, exposure time and long-term corneal side effect of MMC.Universidade Federal de São Paulo, Vis Inst, Dept Ophthalmol, São Paulo, BrazilUniversidade Federal de São Paulo, Vis Inst, Dept Ophthalmol, São Paulo, BrazilWeb of Scienc

Changes in Defoliation Patterns of Plant Functional Groups under Variable Herbage Allowance in Campos Grasslands

Several studies have evaluated separately forage production, botanical composition, leaf traits and animal performance. However, few of them have focused on defoliation patterns at the level of functional groups (FGs) under different and variable herbage allowance (HA), especially in natural, diverse grasslands. The objective was to evaluate the relationship between HA and leaf traits on defoliation patterns of mature beef cows in the autumn, winter and spring. We evaluated the grazing probability (GP), intensity of defoliation (ID), and leaf traits on 14 species that represent more than 80% of total dry matter of the pasture. The experiment at which we evaluated those traits and responses has been managed under High HA (HHA) and Low HA (LHA) (8 and 5 kg dry matter kg live weight-1, respectively). Four plant FGs (A, B, C and D) were defined according to leaf traits, and a selectivity index (SI) was developed for each group (considering the proportion of grazed and ungrazed species). Grazing patterns shifted across seasons. In the autumn, grazing was concentrated on FGs A, B and C groups (GP = 0.417). While for FG D, represented by high-biomass tussocks, the GP was lower (0.075). During winter, when herbage accumulation rate is limited, the average GP was 0.175. FGs C and D were more defoliated in relation to autumn, and during spring the GP shifted to FG B (0.289). The ID was similar to all FGs and seasons (66 % of leaf removed). In autumn and spring, the SI was affected by FGs and HA while in winter were similar between FGs but higher in HHA. Cows behaved differently in the defoliation pattern, modifying mainly the GP on FGs rather than the ID. Variation in HA across season determined changes in defoliation pattern, allowing to express selectivity in autumn and spring

Evaluation of Limpograss (\u3cem\u3eHemarthria altissima\u3c/em\u3e) Breeding Lines under Different Grazing Managements

Limpograss (Hemarthria altissima (Poir.) Stapf et C.E. Hubb.) is a stoloniferous, warm-season perennial grass from South Africa. It is frequently used to extend the grazing season in poorly drained soils of subtropical regions (Quesenberry et al. 2004). The cold tolerance of limpograss allows it to grow at temperatures below which other commonly used warm-season grasses (e.g. bermudagrass) remain productive. Use of limpograss has helped to reduce forage shortfall during winter, therefore, reducing feeding costs. In the past 30 years, the area planted to limpograss in Florida, USA has grown faster than that of any other forage grass species. It is estimated that over 0.2 million ha are planted to limpograss (Quesenberry et al. 2004). Recent University of Florida research with limpograss has focused on developing new hybrids which incorporate the persistence of the most widely used cultivar ‘Floralta’ with the digestibility of ‘Bigalta’. Preliminary clipping and grazing trials evaluated 50 breeding lines and identified 5 lines (designated 1, 4F, 10, 32 and 34) with superior performance. With an overall program goal of identifying the best limpograsses for cultivar release, the specific objective of this experiment was to investigate the forage productivity and sward canopy characteristics of these 5 breeding lines, compared to Floralta, in response to different grazing management strategies

Development of Forage Curriculum for Extension Educators in the Southeast USA

In the Southeast USA, livestock production is one of the largest agricultural activities, and forages are the primary feed source. Most livestock systems are highly dependent on off-farm inputs to support forage production and animal performance, which elevates production costs and the activity\u27s carbon footprint. There is a strong need to develop forage educational resources to enhance productivity, environmental sustainability, resilience, and profitability of agricultural systems in the region. This multi-disciplinary initiative was a collaboration among several land-grant Universities across the region. The objective was to develop decision tools and provide in-classroom training associated with hands-on demonstrations to Extension agents and agricultural educators in the Southeast. A textbook was developed for the two-day training, and the in-person program was held in Columbiana, AL, through a collaboration among 20 Specialists from several land-grant Universities. The book included basic concepts and management strategies for forages, livestock (e.g., beef, horse, small ruminants), soil, economics, nutrient management, animal genetics, and marketing strategies for forage-based systems. There were 62 participants from Alabama, Florida, Georgia, Louisiana, North Carolina, South Carolina, and Tennessee. The majority of the participants were Extension agents or educators, and close to half of them had been in the job position for less than five years (n= 28 participants). A significant emphasis was set on newer agents` participation aiming to allow them to address gaps in knowledge. The training sessions supported critical thinking and deepening of knowledge and network. Post-training surveys were applied to gather change in knowledge and feedback from participants and identify potential barriers to be used in structuring future curriculum development and trainings. Regional joint efforts can be a tool to address multi-disciplinary training while incentivizing collaboration across regions for Specialists and agents through their programming activities

Use of Limpograss as an Alternative Feed During the Fall Forage Gap in Beef and Dairy Systems in Central and North Florida

In central and North Florida, the use of limpograss [Hemarthria altissima (Poir.) Stapf & C. E. Hubb.] for beef and dairy operations is limited and its potential use is not well documented. Two on-farm projects have been conducted in Central and North Florida to explore the use of limpograss as an alternative conserved forage during late fall and winter. The potential use of this forage as baleage for dairy farmers and as stockpiling for livestock producers would offer another alternative to reduce feed costs and fill the forage gap in the area when typical warm-season forages go dormant. In addition, the on-farm limpograss establishment would serve as dissemination for the limpograss planting material. Four dairy farms in Central and North Florida were enrolled in the study to evaluate two cultivars of limpograss for their potential when conserved as baleage. Four 0.2 ha strips were planted per farm, two for each variety (‘Kenhy’ and ‘Gibtuck’). The strips were arranged in a randomized complete block design, with two replicates in each location. Before wrapping the harvested forage for baleage, samples were taken to evaluate crude protein (CP) and in vitro digestible organic matter concentration (IVDOM). In addition, samples of fresh baleage at 60 and 90 d were analyzed for fermentation profile (pH, organic acids, and ammonia). Four beef cattle farms in North Florida allowed us to plant 1 ha of ‘Gibtuck’ for stockpiling evaluating the nutritive value at 30, 60, 90, and 120 days. Each plot was replicated four times and allocated in a randomized complete block design. The fermentation profile from the bales does not show differences between cultivars (P \u3e 0.05) and the pH is lower than 5 indicating that the fermentation process was successful. The CP and IVDOM of the stockpiling limpograss were different among the treatments (P \u3c 0.001)

Diurnal Variation in Forage Nutrient Composition and Metabolic Parameters of Horses Grazing Warm-Season, Perennial Grass-Legume Mixed Pastures

Although warm-season, grass-legume mixed pastures have improved nutritive value and may reduce negative environmental impacts relative to nitrogen-fertilized grass monocultures, no study has been done to evaluate their effect on diurnal variation of non-structural carbohydrates (NSC) and other nutrients, and on the metabolic responses observed in horses’ blood and fecal samples. This 2-yr study aimed to investigate the circadian variation in nutrient composition and the fecal and blood metabolic responses in horses grazing these pastures. Forage, fecal, and blood samples were collected every 28 days at 0600, 1200, 1800, and 0000 h, in two years, for measurement of diurnal variation in forage nutrient composition and fecal and blood metabolites. Forage nutrient composition was affected by time of the day, with digestible energy (DE) and NSC increasing at 1800 h, crude protein decreasing after 1200 h and the fiber components increasing at 1200 h. Fecal lactate and blood insulin were also affected by time of the day. Fecal lactate increased from 0600 to 1200 h. Insulin levels were greater at 1800 than at 0600 h. The increased insulin level followed the increased concentration of NSC in the forage. In conclusion, warm-season, grass-legume mixed pastures show a diurnal pattern in forage nutrient composition, with increased NSC later in the afternoon. However, the metabolic responses observed in this study were not sufficient to predispose horses to metabolic dysregulation. The results also indicate that restricting grazing to the morning may reduce the forage nutritive value, with decreased concentration of DE and increased concentrations of the fiber components, which may decrease diet digestibility

Herbage Responses and Performance of Mature Horses Grazing Warm-Season Perennial Grass-Legume Mixed Pastures

Legume-grass mixtures may be a useful alternative to nitrogen-fertilized grass monocultures, but pasture and animal responses have not been assessed for pastures grazed by horses in Florida. This 2-yr study compared pasture and horse responses of continuously stocked, mixed pastures of rhizoma peanut (RP, Arachis glabrata Benth) and bahiagrass (BG, Paspalum notatum Flüggé) receiving 30 kg nitrogen (N)/ha (RP-BG) compared with BG pastures fertilized with 120 kg/N ha (BG-N) or with no N (BG-No N). Herbage mass was similar among treatments in 2020 and for most evaluation days in 2019. In 2019, stocking rate (AU/ha) was greater in BG-N (3.9) than in RP-BG (3.7) and BG-No N (3.1). In 2020, BG-No N (2.6) had the lesser stocking rate compared with BG-N (2.9) and RP-BG (2.9), with RP-BG not differing from BG-N. Herbage crude protein (CP) and digestible energy were similar across treatments in 2020, but they were greater for BG-N and RP-BG than BG-No N at some evaluation days in 2019. Except for CP, treatment did not affect nutrient digestibility by horses. Digestibility of CP was greatest for RP-BG in the late season. In the RP-BG treatment, proportion of RP in the pasture (~29%) was not affected by sampling date, and RP comprised 18.4% of the diet. Nonetheless, no differences were observed among treatments for body weight and condition score. The results indicate that intercropping legumes into warm-season perennial pastures can improve some measures of nutritive value and maintain horses’ body condition with similar stocking rate as N-fertilized bahiagrass pastures, while contributing to development of sustainable grazing systems for horses with reduced off-farm nitrogen inputs

A Novel Technique to Label Cover Crop Biomass Using Stable Isotopes

Stable isotopes can be used as tracers for carbon and nitrogen pathways being a great tool to track nutrients in integrated systems. The objective of this experiment was to understand the partitioning of 15N and 13C within cover crop plants when they were labeled with stable isotopes, using chambers under field conditions. Cover crops were planted at the University of Florida, North Florida Research and Education Center-Marianna, located in Marianna, FL. Treatments were four cover crops, in which one was considered a typical cover crop system and the other three consisted of an integrated crop-livestock system with or without the inclusion of legume or different nitrogen fertilizer rates grazed every two weeks. All treatments were replicated three times in a randomized complete block design. Two chambers were built and placed in each plot to label the cover crop plants. For the 15N labeling, 15N2-labeled urea (98 atom% 15N) was applied at a rate of 0.5 kg N ha-1 only once. The target amount of 13CO2 (99 atom% 13C) was determined considering a 20% enrichment of the CO2 concentration present inside the chamber’s volume. The 13CO2 labeling was performed for 28 consecutive days. The labeling technique using chambers and stable isotopes to enrich cover crop species worked under field conditions for both, grass and legume species. Moving forward, this labeling technique can be a useful tool to track nutrient pathways, especially litter decomposition in diversified integrated crop and livestock systems under different management practices

Release of a New Forage Bermudagrass Cultivar from the USDA-NPGS Cynodon Collection

Warm-season perennial grasses are the backbone of the pasture-based livestock industry in the southeastern USA. In Florida specifically, bahiagrass (Paspalum notatum Flugge) and bermudagrass (Cynodon spp.) support 1 million head of cattle and 15,000 beef cattle operations. Bermudagrass is the most widely planted forage species in the southeastern USA, planted in approximately 15 million ha and used for grazing, hay and silage. The genus Cynodon is native to southern Africa and germplasm collections have revealed a high degree of genetic variability within the genus. The United States Department of Agriculture National Plant Germplasm System (USDA-NPGS) maintains a collection of bermudagrass plant introduction (PIs) in Griffin, GA, USA and the USDA Georgia Coastal Plains Experiment Station, Tifton, GA, maintains additional forage germplasm. Multi-location trials were established in 2014 in four states (FL, GA, NC and OK) to screen the collection for herbage accumulation (HA) and nutritive value (NV). Due to the large genotype by environment interaction for HA across states, we focused on selecting accessions adapted to South Georgia and Florida. Several PIs showed improved HA and NV compared to ‘Tifton 85’ across several trials and years. Particularly, PI 316510 produced high HA in Citra, FL and Tifton, GA, had improved NV traits, and faster establishment compared to Tifton 85. We confirmed that PI 316510 is tetraploid by chromosome counts and flow cytometry. The PI 316510 has been released by the University of Florida under the name “Newell”