Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from mycobacterium tuberculosis

A number of different interferon-c ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratories use a short in vitro stimulation period of cells before they are transferred to the ELISpot plate; this is commonly done in the case of frozen cells, in order to enhance assay sensitivity. Other differences that may be significant include antibody coating of plates, the use of media with or without serum, the serum source and the number of cells added to the wells. The aim of this paper was to identify which components of the different ELISpot protocols influenced assay sensitivity and inter-laboratory variation. Four laboratories provided protocols for quantifying numbers of interferon-c spot forming cells in human peripheral blood mononuclear cells stimulated with Mycobacterium tuberculosis derived antigens. The differences in the protocols were compared directly. We found that several sources of variation in assay protocols can be eliminated, for example by avoiding serum supplementation and using AIM-V serum free medium. In addition, the number of cells added to ELISpot wells should also be standardised. Importantly, delays in peripheral blood mononuclear cell processing before stimulation had a marked effect on the number of detectable spot forming cells; processing delay thus should be minimised as well as standardised. Finally, a pre-stimulation culture period improved the sensitivity of the assay, however this effect may be both antigen and donor dependent. In conclusion, small differences in ELISpot protocols in routine use can affect the results obtained and care should be given to conditions selected for use in a given study. A pre-stimulation step may improve the sensitivity of the assay, particularly when cells have been previously frozen

Defining user perception of distributed multimedia quality

This article presents the results of a study that explored the human side of the multimedia experience. We propose a model that assesses quality variation from three distinct levels: the network, the media and the content levels; and from two views: the technical and the user perspective. By facilitating parameter variation at each of the quality levels and from each of the perspectives, we were able to examine their impact on user quality perception. Results show that a significant reduction in frame rate does not proportionally reduce the user's understanding of the presentation independent of technical parameters, that multimedia content type significantly impacts user information assimilation, user level of enjoyment, and user perception of quality, and that the device display type impacts user information assimilation and user perception of quality. Finally, to ensure the transfer of information, low-level abstraction (network-level) parameters, such as delay and jitter, should be adapted; to maintain the user's level of enjoyment, high-level abstraction quality parameters (content-level), such as the appropriate use of display screens, should be adapted

A comparative study of the viscoelastic constitutive models for frictionless contact interfaces in solids

The nature of the constitutive contact force law utilized to describe contact-impact events in solid contact interfaces plays a key role in predicting the response of multibody mechanical systems and in the simulation of engineering applications. The goal of this work is to present a comparative study on the most relevant existing viscoelastic contact force models. In the sequel of this process, their fundamental characteristics are examined and their performances evaluated. Models developed based on the Hertz contact theory and augmented with a damping term to accommodate the dissipation of energy during the impact process, which typically is a function of the coefficient of restitution between the contacting solids, are considered in this study. In particular, the identified contact force models are compared in the present study for simple solid impact problems with the sole purpose of comparing the performance of the various models and examining the corresponding system behavior. The outcomes indicate that the prediction of the dynamic behavior of contacting solids strongly depends on the selection of the contact force model.Fundação para a Ciência e a Tecnologia (FCT

Chemotaxonomy of fungi: a review.

For few years, advancements of molecular methods and analytical techniques enabled scientists to realise a classification of microorganisms based on biochemical characteristics. This classification, called chemotaxonomy, includes molecular methods and chemical methods which provide additional data and lead to a better identification and/or classification

Perceptual Bit Allocation For MPEG-2 CBR Video Coding

In this paper we propose a novel bit allocation scheme for MPEG-2 CBR video coding using a model of the early sta- ges of human vision. On the basis of this model we derive a measure of the macroblock activity different from the one proposed in the MPEG-2 test model 5 (TMS). Experiments, obtained by substituting the proposed perceptual activity measure to the one proposed by the MPEG-2 TMS, yield better results among which improvement of the perceptual quality for a fixed bitrate and vice-versa

Revue bibliographique: les méthodes chimiques d'identification et de classification des champignons

Chemotaxonomy of fungi : a review. For few years, advancements of molecular methods and analytical techniques enabled scientists to realise a classification of microorganisms based on biochemical characteristics. This classification, called chemotaxonomy, includes molecular methods and chemical methods which provide additional data and lead to a better identification and/or classification