Development and validation of the Brunel lifestyle physical activity questionnaire

This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.The purpose of the present programme was to develop and validate a theoretically-grounded instrument to measure the planned and unplanned dimensions of lifestyle PA (PPA and UPA; Dunn, Andersen, & Jakicic, 1998). In Study 1, two samples of British adults (Internet: N = 742; paper: N = 563) were used to establish the content validity of the Brunel Lifestyle Physical Activity Questionnaire (BLPAQ). Exploratory factor analysis yielded a two-factor model (UPA and PPA) that produced acceptable fit indices using confirmatory factors analyses with both samples. The purpose of Study 2 was to examine the test-retest reliability of the BLPAQ over 5 weeks using a sample of leisure centre users, university staff members, and university students (N = 337). High correlations were observed between the two administrations (range = .93-.98; p 95%). In Study 3, the BLPAQ was cross-validated using two criterion measures: the Baecke Questionnaire of Habitual Physical Activity (Baecke, Burema, & Frijters, 1982) and the Godin’s Leisure-Time Exercise Questionnaire (Godin & Shephard, 1985). Multiple linear regressions were performed to predict PPA and UPA from the subscales of the two reference measures. The predictive models differed markedly in terms of gender. Subsequently, the sample of 338 British adults was divided into two subsamples, and these were subjected to a cross-validation using the Limits of Agreement (LoA) methodology advocated by Bland and Altman (1986). The agreement plots revealed that both BLPAQ subscales demonstrated acceptable inter-sample agreement when compared to the criterion measures. In Study 4, a series of structural equation models were tested with the aim of predicting PPA and UPA using the variables that constitute the Theory of Planned Behaviour (TPB). The TPB was able to predict PPA but not UPA. The addition of a direct path between past behaviour to UPA did not result in a significant prediction. Further work is required to examine the factorial structure of the PPA subscale and to increase the number of items in the UPA subscale. In sum, the programme has contributed a valid and reliable theory-based measure of PA as well as evidence to support the utility of the TPB in PA research. However, the TPB framework may require the addition of predictors such as past behaviour and actual behavioural control

Composição nutricional de frutos que compõem a dieta de primatas, morcegos e aves.

Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos

Ferramenta para análise e avaliação de riscos no planejamento de projetos de pesquisa e desenvolvimento.

Dissertação (Mestrado em Desenvolvimento de Tecnologia) - Instituto de Tecnologia para o Desenvolvimento em parceria com o Instituto de Engenharia do Paraná, Curitiba

Agência de Informação: opção de acesso usando a internet.

Resumo

Análise e melhoria de processo do Serviço de Atendimento ao Cidadão (SAC) da Embrapa Florestas.

bitstream/CNPF-2009-09/42630/1/Doc156.pdf1 CD-RO

Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid

Aim of this study was to characterize the biochemical and fatty acids composition of follicular fluid collected from follicles of different sizes and in different phases of ovarian cycle in water buffalo farmed in Italy. Ovaries were collected at slaughterhouse during the breeding season; follicular fluid was aspirated dividing samples in small and large follicles (< 6 mm and > 6 mm respectively) and in luteal and follicular phase. Biochemical analysis and gas-chromatography were performed. Biochemical and fatty acids composition were greatly influenced by both follicular dimension and phase of ovarian cycle. Biochemical composition and its variations were in agreement with previously study conducted in buffalo and other species. This is the first report of the fatty acids composition of buffalo follicular fluid. Twenty-two fatty acids were identified in follicular fluid; nine were saturated fatty acids, six monounsatured fatty acids and seven polyunsatured fatty acids. The most dominant fatty acids were linoleic acid, oleic acid, palmitic acid, stearic acid and arachidonic acid. All the identified fatty acids concentrations vary at least because of follicle dimension or phase, with the exception of γ-linoleic acid and arachidonic acid which concentrations remain stable in all classes

A Rare Disorder in an Orphan Disease: Kikuchi Fujimoto Disease in a Young-Adult Patient with Sickle Cell Anemia

no abstrac

The stealth episome: suppression of gene expression on the excised genomic island PPHGI-1 from Pseudomonas syringae pv. phaseolicola

Pseudomonas syringae pv. phaseolicola is the causative agent of halo blight in the common bean, Phaseolus vulgaris. P. syringae pv. phaseolicola race 4 strain 1302A contains the avirulence gene avrPphB (syn. hopAR1), which resides on PPHGI-1, a 106 kb genomic island. Loss of PPHGI-1 from P. syringae pv. phaseolicola 1302A following exposure to the hypersensitive resistance response (HR) leads to the evolution of strains with altered virulence. Here we have used fluorescent protein reporter systems to gain insight into the mobility of PPHGI-1. Confocal imaging of dual-labelled P. syringae pv. phaseolicola 1302A strain, F532 (dsRFP in chromosome and eGFP in PPHGI-1), revealed loss of PPHGI-1::eGFP encoded fluorescence during plant infection and when grown in vitro on extracted leaf apoplastic fluids. Fluorescence-activated cell sorting (FACS) of fluorescent and non-fluorescent PPHGI-1::eGFP F532 populations showed that cells lost fluorescence not only when the GI was deleted, but also when it had excised and was present as a circular episome. In addition to reduced expression of eGFP, quantitative PCR on sub-populations separated by FACS showed that transcription of other genes on PPHGI-1 (avrPphB and xerC) was also greatly reduced in F532 cells harbouring the excised PPHGI-1::eGFP episome. Our results show how virulence determinants located on mobile pathogenicity islands may be hidden from detection by host surveillance systems through the suppression of gene expression in the episomal state

Genetic Analysis of the Individual Contribution to Virulence of the Type III Effector Inventory of Pseudomonas syringae pv. phaseolicola

Several reports have recently contributed to determine the effector inventory of the sequenced strain Pseudomonas syringae pv. phaseolicola (Pph) 1448a. However, the contribution to virulence of most of these effectors remains to be established. Genetic analysis of the contribution to virulence of individual P. syringae effectors has been traditionally hindered by the lack of phenotypes of the corresponding knockout mutants, largely attributed to a high degree of functional redundancy within their effector inventories. In support of this notion, effectors from Pseudomonas syringae pv. tomato (Pto) DC3000 have been classified into redundant effector groups (REGs), analysing virulence of polymutants in the model plant Nicotiana benthamiana. However, using competitive index (CI) as a virulence assay, we were able to establish the individual contribution of AvrPto1PtoDC3000 to Pto DC3000 virulence in tomato, its natural host, even though typically, contribution to virulence of AvrPto1 is only shown in strains also lacking AvrPtoB (also called HopAB2), a member of its REG. This report raised the possibility that even effectors targeting the same defence signalling pathway may have an individual contribution to virulence, and pointed out to CI assays as the means to establish such a contribution for individual effectors. In this work, we have analysed the individual contribution to virulence of the majority of previously uncharacterised Pph 1448a effectors, by monitoring the development of disease symptoms and determining the CI of single knockout mutants at different stages of growth within bean, its natural host. Despite their potential functional redundancy, we have found individual contributions to virulence for six out of the fifteen effectors analysed. In addition, we have analysed the functional relationships between effectors displaying individual contribution to virulence, highlighting the diversity that these relationships may present, and the interest of analysing their functions within the context of the infection