Characterization of Chilling Sensitivity of Tropical and Temperate Grasses

C16-C18 fatty acid composition of some lipids in several species of temperate and tropical grasses were measured. The fatty acid composition of phosphatidylglycerol (PG) and sulfoquinovosyl diglyceride (SQDG) indicated significant differences between temperate and tropical grasses. Especially the differences of the fatty acid composition of PG were remarkable. The unsaturated fatty acid content of PG in tropical grasses was lower than the contents in temperate grasses. These differences constantly appeared through the year. These differences were caused by the low content of polyunsaturated fatty acids, or the low contents of C18 fatty acids

Classification and stability of simple homoclinic cycles in R^5

The paper presents a complete study of simple homoclinic cycles in R^5. We find all symmetry groups Gamma such that a Gamma-equivariant dynamical system in R^5 can possess a simple homoclinic cycle. We introduce a classification of simple homoclinic cycles in R^n based on the action of the system symmetry group. For systems in R^5, we list all classes of simple homoclinic cycles. For each class, we derive necessary and sufficient conditions for asymptotic stability and fragmentary asymptotic stability in terms of eigenvalues of linearisation near the steady state involved in the cycle. For any action of the groups Gamma which can give rise to a simple homoclinic cycle, we list classes to which the respective homoclinic cycles belong, thus determining conditions for asymptotic stability of these cycles.Comment: 34 pp., 4 tables, 30 references. Submitted to Nonlinearit

Experimental Study of Slat Noise from 30P30N Three-Element High-Lift Airfoil in JAXA Hard-Wall Low-Speed Wind Tunnel

Aeroacoustic measurements associated with noise radiation from the leading edge slat of the canonical, unswept 30P30N three-element high-lift airfoil configuration have been obtained in a 2 m x 2 m hard-wall wind tunnel at the Japan Aerospace Exploration Agency (JAXA). Performed as part of a collaborative effort on airframe noise between JAXA and the National Aeronautics and Space Administration (NASA), the model geometry and majority of instrumentation details are identical to a NASA model with the exception of a larger span. For an angle of attack up to 10 degrees, the mean surface Cp distributions agree well with free-air computational fluid dynamics predictions corresponding to a corrected angle of attack. After employing suitable acoustic treatment for the brackets and end-wall effects, an approximately 2D noise source map is obtained from microphone array measurements, thus supporting the feasibility of generating a measurement database that can be used for comparison with free-air numerical simulations. Both surface pressure spectra obtained via KuliteTM transducers and the acoustic spectra derived from microphone array measurements display a mixture of a broad band component and narrow-band peaks (NBPs), both of which are most intense at the lower angles of attack and become progressively weaker as the angle of attack is increased. The NBPs exhibit a substantially higher spanwise coherence in comparison to the broadband portion of the spectrum and, hence, confirm the trends observed in previous numerical simulations. Somewhat surprisingly, measurements show that the presence of trip dots between the stagnation point and slat cusp enhances the NBP levels rather than mitigating them as found in a previous experiment

Chiral gravity in higher dimensions

We construct a chiral theory of gravity in 7 and 8 dimensions, which are equivalent to Einstein-Cartan theory using less variables. In these dimensions, we can construct such higher dimensional chiral gravity because of the existence of gravitational instanton. The octonionic-valued variables in the theory represent the deviation from the gravitational instanton, and from their non-associativity, prevents the theory to be SO(n) gauge invariant. Still the chiral gravity holds G_2 (7-D), and Spin(7) (8-D) gauge symmetry.Comment: 18 pages, no figures. Minor typos corrected. Updated reference

Wet and dry deposition of mineral dust particles in Japan: factors related to temporal variation and spatial distribution

Recent ground networks and satellite remote-sensing observations have provided useful data related to spatial and vertical distributions of mineral dust particles in the atmosphere. However, measurements of temporal variations and spatial distributions of mineral dust deposition fluxes are limited in terms of their duration, location, and processes of deposition. To ascertain temporal variations and spatial distributions of mineral dust deposition using wet and dry processes, weekly deposition samples were obtained at Sapporo, Toyama, Nagoya, Tottori, Fukuoka, and Cape Hedo (Okinawa) in Japan during October 2008–December 2010 using automatic wet and dry separating samplers. Mineral dust weights in water-insoluble residue were estimated from Fe contents measured using an X-ray fluorescence analyser. Wet and dry deposition fluxes of mineral dusts were both high in spring and low in summer, showing similar seasonal variations to frequency of aeolian dust events (Kosa) in Japan. For wet deposition, highest and lowest annual dust fluxes were found at Toyama (9.6 g m⁻² yr⁻¹) and at Cape Hedo (1.7 g m⁻² yr⁻¹) as average values in 2009 and 2010. Higher wet deposition fluxes were observed at Toyama and Tottori, where frequent precipitation (> 60% days per month) was observed during dusty seasons. For dry deposition among Toyama, Tottori, Fukuoka, and Cape Hedo, the highest and lowest annual dust fluxes were found respectively at Fukuoka (5.2 g m⁻² yr⁻¹) and at Cape Hedo (2.0 g m⁻² yr⁻¹) as average values in 2009 and 2010. The average ratio of wet and dry deposition fluxes was the highest at Toyama (3.3) and the lowest at Hedo (0.82), showing a larger contribution of the dry process at western sites, probably because of the distance from desert source regions and because of the effectiveness of the wet process in the dusty season. <br><br> Size distributions of refractory dust particles were obtained using four-stage filtration: > 20, > 10, > 5, and > 1 μm diameter. Weight fractions of the sum of > 20 μm and 10–20 μm (giant fraction) were higher than 50% for most of the event samples. Irrespective of the deposition type, the giant dust fractions generally decreased with increasing distance from the source area, suggesting the selective depletion of larger giant particles during atmospheric transport. Based on temporal variations of PM_c (2.5 < <i>D</i> < 10 μm), ground-based lidar, backward air trajectories, and vertical profiles of potential temperatures, transport processes of dust particles are discussed for events with high-deposition and low-deposition flux with high PM_c. Low dry dust depositions with high PM_c concentrations were observed under stronger (5 K km⁻¹) stratification of potential temperature with thinner and lower (< 2 km) dust distributions because the PM_c fraction of dust particles only survived after depletion of giant dust particles by rapid gravitational settling at the time they reach Japan. In contrast, transport through a thicker (> 2 km) dust layer with weak vertical gradient of potential temperature carry more giant dust particles to Japan. Because giant dust particles are an important mass fraction of dust accumulation, especially in the North Pacific, which is known as a high-nutrient, low-chlorophyll (HNLC) region, the transport height and fraction of giant dust particles are important factors for studying dust budgets in the atmosphere and their role in biogeochemical cycles

The side chain of glutamine 13 is the acyl-donor amino acid modified by type 2 transglutaminase in subunit T of the native rabbit skeletal muscle troponin complex.

Subunit T of the native muscle troponin complex is a recognised substrate of transglutaminase both in vitro and in situ with formation of isopeptide bonds. Using a proteomic approach, we have now determined the precise site of in vitro labelling of the protein. A preparation of troponin purified from ether powder from mixed rabbit skeletal muscles was employed as transglutaminase substrate. The only isoform TnT2F present in our preparation was recognised as acyl-substrate by human type 2 transglutaminase which specifically modified glutamine 13 in the N-terminal region. During the reaction, the troponin protein complex was polymerized. Results are discussed in relation to the structure of the troponin T subunit, in the light of the role of troponins in skeletal and cardiac muscle diseases, and to the rules governing glutamine side chain selection by tissue transglutaminase

Changes in protein expression in two cholangiocarcinoma cell lines undergoing formation of multicellular tumor spheroids In vitro

Epithelial-to-Mesenchymal Transition (EMT) is relevant in malignant growth and frequently correlates with worsening disease progression due to its implications in metastases and re- sistance to therapeutic interventions. Although EMT is known to occur in several types of solid tumors, the information concerning tumors arising from the epithelia of the bile tract is still limited. In order to approach the problem of EMT in cholangiocarcinoma, we decided to investigate the changes in protein expression occurring in two cell lines under conditions leading to growth as adherent monolayers or to formation of multicellular tumor spheroids (MCTS), which are considered culture models that better mimic the growth characteristics of in-vivo solid tumors. In our system, changes in phenotypes occur with only a decrease in transmembrane E-cadherin and vimentin expression, minor changes in the transglutami- nase protein/activity but with significant differences in the proteome profiles, with declining and increasing expression in 6 and in 16 proteins identified by mass spectrometry. The aris- ing protein patterns were analyzed based on canonical pathways and network analysis. These results suggest that significant metabolic rearrangements occur during the conver- sion of cholangiocarcinomas cells to the MCTS phenotype, which most likely affect the car- bohydrate metabolism, protein folding, cytoskeletal activity, and tissue sensitivity to oxygen

Influence of clay properties on shoe-kinematics and friction during tennis movements

Tennis is a sport characterised by being played on different surfaces: hard court, grass and clay. These surfaces influence the style of play and tennis specific movements. Specifically on clay, most of the common movements performed by players (e.g. accelerating, side stepping and braking), are performed with some level of controlled sliding. In order to reduce the player's injury risk, and assess the shoe-surface requirements on clay surfaces, there is a need for a scientific understanding of the player's kinematics and tribological mechanisms occurring at the shoe-surface interface. The purpose of this study was to identify the kinematics of the shoe during the sliding phase, and to assess the friction that is present. Baseline areas of both ends of a clay court were prepared with two different mixes of clay, varying the particle size. Eight experienced clay players participated in this study which took place during the Conde de Godó tennis tournament in Barcelona, Spain. 3D kinematic data data was collected using two synchronised high speed video cameras, and after the tests, perception questionnaires were applied to the players. Additionally, three different mechanical devices were utilised to measure the friction of the two clay surfaces. Displacement and velocity data of the shoe in contact with the surface were correlated with the friction measurements from both clay surfaces. Results indicated that significant differences occurred between the two clay surfaces for some shoe kinematic data, and mechanical friction. However, the perception scores suggest the opposite behaviour stated by the mechanical test and shoe-kinematic data. The present study has provided evidence that shoe kinematics and friction of the shoe-surface interaction are affected by the surface conditions, specifically particle size