AT THE TABLE; AN INVESTIGATION OF HOW GROWING, COOKING, AND SHARING FOOD TOGETHER CAN BRING A WEALTH OF BENEFITS

MOTIVATION: The value of family mealtime has been well documented by decades of academic research. Children from families, (regardless of race, class or income), that routinely sit down to a meal together, suffer less depression, obesity and substance abuse. They also stay healthier and do better in school (Benefits 2018). There are nutrition, health, social, and mental benefits to eating with others. Research has shown that people eat more fruits and vegetables and other nutrient-rich foods when they share a meal with others. They also drink less soda and eat less fried foods (Benefits 2018). Eating meals together teaches children better communication skills and the opportunity to learn more words (Benefits 2018). PROBLEM: In environments that have limited fresh fruits and vegetables, yet numerous sweet and salty snack food, food insufficiency, and infrequent family meals have been found to be associated with poor dietary intake and/or obesity. (Mason 2014). People and families may make decisions based on their environment or community. For example, a person may choose not to walk or bike to the store or to work because of a lack of sidewalks or safe bike trails. Community, home, child care, school, health care, and workplace settings can all influence people’s daily behaviors. Therefore, it is important to create environments in these locations that make it easier to engage in physical activity and eat a healthy diet (Adult 2018). If we know that eating nutritious meals together at home equals can reduce stress, obesity, and depression, and lead to a happier life, why do people still make other choices? Lack of food education? Resources? Time? METHODS: Direct observational and objective data was collected through a survey to better understand the choices that people make. Research through articles, books, and documentaries will support my findings on the benefits of community kitchens and gardens. Precedents include Shalom Farms, Feed More, and other community kitchens in the country RESULTS: Despite intense nationwide efforts to improve healthy eating, progress has plateaued, and health biases remain (Berge 2017). Community kitchens have been associated with enhanced food skills, improved community food security, and improved social interactions (Iacovou 2013). Studies of community kitchen-based nutrition and cooking instruction program for parents and children suggests increased enjoyment of cooking and decreased consumption of meals away from home (Iacovou 2013). REFLECTIONS & CONCLUSIONS: How might a community cooking school, garden, and table where members share knowledge, resources, and labor to prepare, cook, and consume food improve the member’s health? A kitchen-based nutrition and cooking instruction program for parents and children would bring food freedom, or the right to food, implying that sufficient food is available, that people have the means to access it, and that it adequately meets the individual’s dietary needs and an environment to learn basic cooking techniques and food gardening. This community cooking school and garden will highlight healthful eating, incorporating young children into growing their own produce, cooking, and emphasizing the emotional and social benefits of family meal time. In this space, a variety of programs for all experience and income levels would be available year round. Every class would end with a meal around the table, because eating together is as important as what’s on the plate

How and when do big data investments pay off? The role of marketing affordances and service innovation

Big data technologies and analytics enable new digital services and are often associated with superior performance. However, firms investing in big data often fail to attain those advantages. To answer the questions of how and when big data pay off, marketing scholars need new theoretical approaches and empirical tools that account for the digitized world. Building on affordance theory, the authors develop a novel, conceptually rigorous, and practice-oriented framework of the impact of big data investments on service innovation and performance. Affordances represent action possibilities, namely what individuals or organizations with certain goals and capabilities can do with a technology. The authors conceptualize and operationalize three important big data marketing affordances: customer behavior pattern spotting, real-time market responsiveness, and data-driven market ambidexterity. The empirical analysis establishes construct validity and offers a preliminary nomological test of direct, indirect, and conditional effects of big data marketing affordances on perceived big data performance

Estudio comparativo del sellado total de tres selladores

Buscando el éxito de la terapia endodóntica sabemos que uno de los ítems más difíciles dentro de nuestra especialidad es lograr la hermeticidad del conducto; a través de un sellador ideal. Hoy día seguimos buscando un sellador que cumpla con todos los requisitos esperados Objetivo: El objetivo de este trabajo fue estudiar en forma comparativa el sellado en la totalidad del conducto por medio de la técnica de transparentación. Los selladores endodónticos utilizados en esta oportunidad son el cemento de Grossman, el sellador de CPM Sealer y Roeko Seal.Facultad de Odontologí

The Folding Mechanism of the SH3 Domain from Grb2

SH3 domains are small protein modules involved in the regulation of important cellular pathways. These domains mediate protein-protein interactions recognizing motifs rich in proline on the target protein. The SH3 domain from Grb2 (Grb2-SH3) presents the typical structure of an SH3 domain composed of two-three stranded antiparallel \u3b2-sheets orthogonally packed onto each other, to form a single hydrophobic core. Grb2 interacts, via SH3 domain, with Gab2, a scaffolding disordered protein, triggering some key metabolic pathways involved in cell death and differentiation. In this work we report a mutational analysis (\u3c6-value analysis) of the folding pathway of Grb2-SH3 that, coupled with molecular dynamic simulations, allows us to assess the structure of the transition state and the mechanism of folding of this domain. Data suggest that Grb2-SH3 folds via a native-like, diffused transition state with a concurrent formation of native-like secondary and tertiary structure (nucleation-condensation mechanism) and without the accumulation of folding intermediates. The comparison between our data and previous folding studies on SH3 domains belonging to other proteins, highlights that proteins of this class may fold via alternative pathways, stabilized by different nuclei leading or not to accumulation of folding intermediates. This comparative analysis suggests that the alternative folding pathways for this class of SH3 domains can be selectively regulated by the specific aminoacid sequences

Estudio comparativo del sellado total de tres selladores

Buscando el éxito de la terapia endodóntica sabemos que uno de los ítems más difíciles dentro de nuestra especialidad es lograr la hermeticidad del conducto; a través de un sellador ideal. Hoy día seguimos buscando un sellador que cumpla con todos los requisitos esperados Objetivo: El objetivo de este trabajo fue estudiar en forma comparativa el sellado en la totalidad del conducto por medio de la técnica de transparentación. Los selladores endodónticos utilizados en esta oportunidad son el cemento de Grossman, el sellador de CPM Sealer y Roeko Seal.Facultad de Odontologí

Estudio comparativo del sellado total de tres selladores

Buscando el éxito de la terapia endodóntica sabemos que uno de los ítems más difíciles dentro de nuestra especialidad es lograr la hermeticidad del conducto; a través de un sellador ideal. Hoy día seguimos buscando un sellador que cumpla con todos los requisitos esperados Objetivo: El objetivo de este trabajo fue estudiar en forma comparativa el sellado en la totalidad del conducto por medio de la técnica de transparentación. Los selladores endodónticos utilizados en esta oportunidad son el cemento de Grossman, el sellador de CPM Sealer y Roeko Seal.Facultad de Odontologí

Templated folding of intrinsically disordered proteins

Much of our current knowledge of biological chemistry is founded in the structure-function relationship, whereby sequence determines structure that determines function. Thus, the discovery that a large fraction of the proteome is intrinsically disordered, while being functional, has revolutionized our understanding of proteins and raised new and interesting questions. Many intrinsically disordered proteins (IDPs) have been determined to undergo a disorder-to-order transition when recognizing their physiological partners, suggesting that their mechanisms of folding are intrinsically different from those observed in globular proteins. However, IDPs also follow some of the classic paradigms established for globular proteins, pointing to important similarities in their behavior. In this review, we compare and contrast the folding mechanisms of globular proteins with the emerging features of binding-induced folding of intrinsically disordered proteins. Specifically, whereas disorder-to-order transitions of intrinsically disordered proteins appear to follow rules of globular protein folding, such as the cooperative nature of the reaction, their folding pathways are remarkably more malleable, due to the heterogeneous nature of their folding nuclei, as probed by analysis of linear free-energy relationship plots. These insights have led to a new model for the disorder-to-order transition in IDPs termed “templated folding,” whereby the binding partner dictates distinct structural transitions en route to product, while ensuring a cooperative folding

Hidden kinetic traps in multidomain folding highlight the presence of a misfolded but functionally competent intermediate

Although more than 75% of the proteome is composed of multidomain proteins, current knowledge of protein folding is based primarily on studies of isolated domains. In this work, we describe the folding mechanism of a multidomain tandem construct comprising two distinct covalently bound PDZ domains belonging to a protein called Whirlin, a scaffolding protein of the hearing apparatus. In particular, via a synergy between NMR and kinetic experiments, we demonstrate the presence of a misfolded intermediate that competes with productive folding. In agreement with the view that tandem domain swapping is a potential source of transient misfolding, we demonstrate that such a kinetic trap retains native-like functional activity, as shown by the preserved ability to bind its physiological ligand. Thus, despite the general knowledge that protein misfolding is intimately associated with dysfunction and diseases, we provide a direct example of a functionally competent misfolded state. Remarkably, a bioinformatics analysis of the amino acidic sequence of Whirlin from different species suggests that the tendency to perform tandem domain swapping between PDZ1 and PDZ2 is highly conserved, as demonstrated by their unexpectedly high sequence identity. On the basis of these observations, we discuss on a possible physiological role of such misfolded intermediate