Tes Dna dalam Menetapkan Hubungan Nasab

Deoxirybo Nucleic Acid (DNA) is a great scientific discovery. It constitutes the main chemical elements that build up gens as a prime unit for shaping of biological and genealogical traits of ascendants to their descendants. In its later development, DNA is used for various purposes ranging from personal to legal matters, such as determining genealogy to decide legal status regarding child guardianship, adoption, inheritance or other purposes such as forensic research. DNA is a new discovery which was unknown in the early period of the formulation of Islamic law during the lifetimes of the Prophet Muhammad and his Companions. At the time, it was alqiyafah that was used to determine people's genealogy through their foot palm or traces. Now DNA can help resolve a doubt concerning one's genealogy. Using an Islamic perspective, this articles aims to analyze DNA as the method of determining genealogy. It argues that although DNA is a valid method, and perhaps more precise than any previous method, it still needs additional proofs such as testimony and witness. These additional proofs are needed in case any error occurs during the processes of DNA examination

Development of a Forward/Adjoint Hybrid Monte Carlo Absorbed Dose Calculational Method for Use in Radiation Therapy

A successful radiation therapy treatment aims at conforming (i.e., concentrating) radiation dose to the entire tumor volume (i.e., diseased area) while avoiding surrounding normal tissue (i.e., healthy non-diseased areas). This objective is achieved clinically by finding a set of radiation beam parameters that successfully deliver the desired dose distribution. In this project, a hybrid forward/adjoint Monte Carlo based absorbed dose computation method is developed and tested, aimed at eventual implementation in a radiation therapy external beam treatment planning system to predict the absorbed dose produced by a medical linear accelerator. This absorbed dose calculational engine was designed to be: Efficient. This is achieved by incorporating several Monte Carlo techniques used in the Nuclear Engineering field for deep penetration and reactor analysis problem. Flexible. This is achieved by using a Cartesian grid and a voxelized material map. Currently most of the absorbed dose calculation algorithms in radiotherapy are 3-D based predictive models. The use of such algorithms results in treatment planning quality that depends tremendously on the planner’s experience and knowledge base. This dependence, along with inaccuracy in predicting absorbed dose due to the assumptions and simplifications used in these algorithms, can result in a predicted absorbed dose that under- or over-predicts the delivered dose. As an alternative, forward and adjoint Monte Carlo absorbed dose computation methods have been used and validated by several authors (Difilippo, 1998; Goldstein & Regev, 1999; Jeraj & Keall, 1999). However, in the “pure” forward or adjoint methods, each change in the radiation beam parameters requires its own time-consuming 3D calculation; for the hybrid technique developed in this research, a single 3D calculation for each desired dose region (tumor or healthy organ) is all that is required. This project also improves the Monte Carlo methodology by incorporating the use of voxelized fictitious scattering and surface forward/adjoint coupling. The accuracy is demonstrated through comparison with forward and adjoint MCNP calculations of a simple beam/patient sample problem

Asthma diagnosis and treatment - 1012. The efficacy of budesonide in the treatmetn of acute asthma in children: a double-blind, randomized, controlled trial.

Background Current evidence suggests that inhaled glucocorticoids (IGC) have a more profound topical none genomic effect on bronchial airways as compared to systemic glucocorticoids. The value of adding IGC to current therapy of acute asthma is not well established. Methods We conducted a double-blind, randomized, two-arm, parallel groups, controlled clinical trial to compare the addition of budesonide 1500 mcg or placebo (normal saline) to standard acute asthma treatment (albuterol and ipratropium bromide) administered in 3 divided mixed doses within 1 hour in the emergency department (ED). Children 2-12 years of age with moderate or severe acute asthma, scoring 8-15/15 on a well-validated scoring system were included. Both groups received a single dose of prednisone 2 mg/kg/day (max. 60 mg) at the beginning of therapy. The primary outcome was admission rate within 2-4 hours from starting therapy. Results A total of 723 children were enrolled in the study over 17 months duration, of whom 139 were allowed to re-enroll and be randomized to constitute 906 randomization assignments (458 on the treatment group and 448 on the control group); with baseline mean + SD asthma score of 10.63 + 1.73; age 5.52 + 2.76 years; 35% girls; 30.8% (16.5%) with baseline severe asthma score of ≥12 (≥ 13). Statistical Analysis plan allowed for the potential dependency in response due to reenrollments of a subset of children, using Generalized Linear Mixed Modeling (GLMM) techniques. Baseline demographic and clinical characteristics were not significantly different between the two randomized groups. Seventy-five out of 458 (16.4%) of the treatment group vs. 82/448 (18.3%) of the control group were admitted, (OR 0.85, CI: 0.59-1.23, p-value=0.39). Among the severe asthmatics with baseline score ≥13, treatment vs. placebo group, GLMM adjusted admission rate was 30% vs. 47%, indicating a 17% difference in admission rate in favor of the treatment group (adjusted OR of 0.49, CI: 0.25-0.95; p-value= 0.035) that indicated a 51% reduction in the risk of admission for the treatment vs. control group. Conclusions Children with baseline severe asthma score ≥13 who were treated with budesonide had a significant reduction in their admission rate

Modification of chitin as substrates for chitinase

Enzymes are able to bind to their substrates specifically at the active site. The proximity and orientation of the substrates strongly increase the likelihood that productive E–S complexes will arise. Treated chitin (powder or flake) is more efficient than crystalline chitin. This is because the latter is less active due to its insolubility. The structure of treated chitin is opened; this facilitates its interaction with the enzyme. The purpose of this research was to create a kind of modified chitin and study the characterization of the different types of chitin including functional groups by IR spectrophotometer, pore size, surface area and crystallinity by X-Ray diffraction. Chitin from shrimp shell was modified into colloidal, bead, amorphous and superfine chitin. The results of the IR spectra of colloidal and bead chitin showed a similar pattern with chitin powder; they peaked at 3447 and 3113 cm-1 (OH and NH2 groups), 1645 cm-1 (amide groups N-H) and 1071 cm-1 (group C-O). Superfine and amorphous chitin had similar absorbance with powder chitin but appeared to peak in the fingerprint region. Characterization of physical properties based on the pore size and surface area of powder, colloidal, superfine, amorphous and bead chitin changed the pore radius of each type of chitin due to the treatment of swelling. Crystallinity showed that specific diffractogram pattern in the three main peaks 2q was 9.5, 19.5 and 26 with varying intensity. Chitinase activity assay using modified types of chitin substrate had higher values than chitin powder. The highest activity was in amorphous chitin with values of 1.858 U/mL. This is because it has chitin chain and the rearrangement of its structure was more open, facilitating its interaction with enzyme.Keywords: Chitin modified, chitinase, substrate.Abbreviation: SDS, Sodium dodecyl sulfate; Ct, chitin powder; Cc, colloidal chitin; Sf, superfine; Cb, bead; Ca, amorphous

Synthesis and Characterization of New Polyimide Contain Heterocyclic

Novel heterocyclic polyimide 5(a,b) have been synthesized based on polyacrylic backbone. The synthetic route start with nucleophilic substitution of 2-amino, or 4-amino, pyridine 1(a,b) to the polyacryloyl chloride afforded poly substituted amide 2(a,b). Another nucleophilic substitution were carried with adipoyl chloride to form polyimide chloride 3(a,b). Treatment of 3(a,b) with hydrazine hydrate afforded acid hydrazide polyimide 4(a,b), which upon cyclocondensation with carbon disulfide gave the target heterocyclic polyimide. The synthesized compounds were identified by spectroscopic methods: FT-IR, 1H-NMR and 13C-NMR

Mammographic density as a mediator for breast cancer risk: analytic approaches

Mammographic breast density has been found to be associated with breast cancer risk. Many of the traditional risk factors for breast cancer are themselves associated with mammographic breast density. A natural question that arises in this setting is the extent to which the effects of breast cancer risk factors are mediated by breast density and the extent to which such effects are through other pathways. We discuss analytic approaches to address these questions of mediation and also discuss how such approaches can accommodate potential interaction between risk factors and mammographic density and can accommodate case-control study designs

Gene × Gene interaction between MnSOD and GPX-1 and breast cancer risk: a nested case-control study

BACKGROUND: Germ-line mutations in genes such as BRCA1, BRCA2, and ATM can cause a substantial increase in risk of breast cancer. However, these mutations are rare in the general population, and account for little of the incidence of sporadic breast cancer in the general population. Therefore, research has been focused on examining associations between common polymorphisms and breast cancer risk. To date, few associations have been described. This has led to the hypothesis that breast cancer is a complex disease, whereby a constellation of very low penetrance alleles need to be carried to present a risk phenotype. Polymorphisms in the manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPX-1) genes have been proposed as low penetrance alleles, and have not been clearly associated with breast cancer. We investigated whether variants at both polymorphisms, while not independently associated with breast cancer risk, could influence breast cancer risk when considered together. METHODS: A case-control study nested within the Nurses' Health Study was performed comparing 1262 women diagnosed with breast cancer to 1533 disease free women. The MnSOD (Val16Ala, rs1799725) and GPX-1 (Pro198Leu, rs1050450) were genotyped via TaqMan assay. Disease risk was evaluated using logistic regression. RESULTS: While neither allele alone shows any change in breast cancer risk, an increase in the risk of breast cancer (OR 1.87, 95% CI 1.09 – 3.19) is observed in individuals who carry both the Ala16Ala genotype of MnSOD and the Leu198Leu genotype of GPX-1. CONCLUSION: Polymorphisms in the GPX-1 and MnSOD genes are associated with an increased risk of breast cancer

Photodegradation of 2-naphthol Using Nanocrystalline TiO2

The kinetics of the photocatalytic degradation of 2-naphthol has been investigated in aqueous suspensions of titanium dioxide (TiO2) under a variety of conditions, which is essential from application point of view. The degradation was studied using different parameters such as types of TiO2, catalyst concentration, substrate concentration, reaction pH and in the presence of different electron acceptors such as hydrogen peroxide (H2O2), potassium bromate (KBrO3) and potassium persulphate (K2S2O8) besides molecular oxygen. The degradation rates were found to be strongly influenced by all the above parameters. The photocatlyst ″Degussa P-25″ was found to be more efficient as compared with other photocatalysts. The results indicate the process follows Langmuir-Hinshelwood-type kinetics and inference is made of the reaction taking place on the semiconductor particle surface. An analysis of Total Organic Carbon (TOC) showed that a complete mineralization of 2-naphthol can be easily achieved.The kinetics of the photocatalytic degradation of 2-naphthol has been investigated in aqueous suspensions of titanium dioxide (TiO2) under a variety of conditions, which is essential from application point of view. The degradation was studied using different parameters such as types of TiO2, catalyst concentration, substrate concentration, reaction pH and in the presence of different electron acceptors such as hydrogen peroxide (H2O2), potassium bromate (KBrO3) and potassium persulphate (K2S2O8) besides molecular oxygen. The degradation rates were found to be strongly influenced by all the above parameters. The photocatlyst ″Degussa P-25″ was found to be more efficient as compared with other photocatalysts. The results indicate the process follows Langmuir-Hinshelwood-type kinetics and inference is made of the reaction taking place on the semiconductor particle surface. An analysis of Total Organic Carbon (TOC) showed that a complete mineralization of 2-naphthol can be easily achieved

Synthesis, Characterization and Antibacterial Activity of Cefalexin Dervatives

New series of Schiff bases 2(a-j) and corresponding beta-lactam derivatives 3(a-j) were synthesized from cefalexin (1) as starting material. The compound (1) was reacted with different aldehydes and ketones to give Schiff bases derivatives 2(a-j). The synthesized Schiff bases were cyclized by chloroacetyl chloride in the presence of triethylamine to form beta-lactam derivatives 3(a-j). The compounds were characterized by deremination melting point, FT-IR and 1H NMR. The beta-lactam derivatives were screened in vitro antibacterial against some bacterial specie