RP-HPLC QUANTIFIABLE TECHNIQUE DEVELOPMENT FOR EVALUATING PREGABALIN AND ETORICOXIB COMBINATION IN TABLET AND BULK KINDS

Objective: This evaluation study aims to initiate a relatively sensitive RP-HPLC quantifiable technique for evaluating pregabalin (PRBN) and etoricoxib (ETRB) combination in tablet and bulk kinds. Methods: PRBN and ETRB chromatographic evaluations were carried off using the “KNAUER C18 Eurospher II column (250 mm × 4.6 mm × 5 μ)”. The mobile phase (MBP) was driven into the KNAUER C18 Eurospher II column at a 1.0 ml/min run rate with an isocratic elution programme of 65% volume of 0.5 mmol sodium perchlorate 35% volume methanol, detected and evaluated the PRBN and ETRB content at 217 nm. Results: The analysis of PRBN and ETRB is executed inside a run period of 15 min. The RP-HPLC quantifiable technique was developed to separate PRBN and ETRB and likely degradants formed from stress testing by isocratic elution. The RP-HPLC quantifiable technique developed was successfully validated to existing ICH limit guidelines and was confirmed as robust, specific, accurate, selective, precise, sensitive, and linear. Conclusion: The RP-HPLC quantifiable technique developed here is more valuable and worthy for routine PRBN and ETRB analysis of tablets and bulk kinds

Applying Machine Learning Techniques to Categorize and Reduce Stress in Human Beings

The number of individuals in the modernworld experience elevated stress level, which is non-specific response on the body and plays a significant toll on health, productivity at work, relationships and also effect overall well-being. Many individuals are not aware of the stress triggers and potential health problems caused by prolonged stress. In order to effectively combat stress and its ill effects on health, stress triggers and responses to stress must be recognized and managed in real time. In this paper, applications of machine learning techniques are suggested to categorize and reduce stress is explored. The idea of monitoring stress and reducingstress usesmethods like personalized music, wallpaper themes, favorite games or favorite food ordering and so on. Activities which reduce stress and their degree of reduction are monitored in real time and based on customized stress reduction portfolio is designed using machine learning algorithms

Improving Vocabulary and Pronunciation of Secondary Level ESL Learners Through Language and Meaning-Focused Listening- An Experimental Study

Listening plays a larger role in language acquisition and is a critical aim for students in order to receive information generated in the target language. Its successful acquisition requires students to absorb cognitive processes and then use them in their spoken interactions in order to promote understanding in a variety of communication settings. It is the instructor's job to choose the most effective methods for ensuring students' effective learning of this language ability by selecting projects and resources that best meet their unique and linguistic learning requirements and interests. The main purpose of this research paper is to emphasize the critical role of Language-focused and Meaning-focused listening in the process of English as a second language (ESL) learning through an experimental study. Since, listening skill contributes a major part in the enhancement of ESL learners’ language proficiency, the feasible activities and strategies provided in this paper improve the vocabulary and pronunciation of secondary level ESL learners. The paper also discusses the processes of listening skill: top-down and bottom-up methods to emphasize the enhancement of ESL students’ vocabulary and pronunciation. The researcher has chosen grade 9 students of a government school as a sample for the experiment

An explorative clinical study to evaluate the combined effect of Shiropichu, Sthanika Abhyanga, Salvana Upanaha Sweda and Balarishta in the management of Pakshaghata vis~a~vis Hemiplegia

Pakshaghata is one among the Vata Nanatmaja Vyadhi, characterised by loss of function either in right or left half of the body. Pakshaghata is also considered among the ailments of Madhyama Roga Marga. Signs and symptoms of Pakshaghata is similar to that of stroke with hemiplegia as the cardinal clinical feature. It makes the affected person physically disabled and reduces quality of life. Even though western science had moved for in acute management of stroke, it is very difficult to handle the residual effect of stroke which makes person physically and mentally debilitated. In case of managing residual cases, it depends on physiotherapy and exercises, which gives good results if combined with Ayurvedic treatments such as Abhyanga. Present study is a single group open clinical trial with pre and post-test design with a sample size of 30 subjects. Subjects were administered with Shiropichu, Sthanika Abhyanga, Salvana Upana Sweda for first 10 consecutive days and Shamanaaushadha for all 30 days. The results obtained on parameter muscle power, muscle tone, finger movements and deep tendon reflexes was statistically non-significant in the management of Pakshaghata vis-à-vis hemiplegia. The results obtained on parameter stroke specific quality of life scale showed statistically significant result with p value 0.005 in the management of Pakshaghata vis-à-vis hemiplegia. Hence, it was inferred that the intervention selected for the present study possess combined effect in improving the quality of life of individual affected with Pakshaghata vis-à-vis Hemiplegia

Standardization of optimum sieve size for maximizing seed quality in Amaranthus (Amaranthus tricolor L.)

Vegetables have a critical function in human health and nutritional security. Vegetables are considered an essential building block of any diet. Out of the leafy vegetables, Amaranthus is the most popular and salable vegetable consumed by people all over India. Seed processing experiment was undertaken in Amaranthus tricolor (CO 2) by using the sieves placed inside the mechanical seed shaker (Gyratory sieve shaker) to improve the quality of Amaranthus seeds. The seeds of Amaranthus were size graded with seed shaker attached with various sieve size of BSS 18 X18 (R), BSS 20 X 20 (R), BSS 22 X 22 (R) and BSP 22 X 22 (P). During processing, the machine could be adjusted for 2, 3, 4 and 5 minutes with an oscillating speed of 1440 rpm. The separated seeds were evaluated for seed quality characteristics such as seed germination percentage, shoot length, root length, dry matter production, 1000 seed weight and seed recovery percentage. The results revealed that the sieve size of BSS 22 X 22 (R=retained) mesh sieve had the better quality seeds with a maximum recovery of 67.41 g and when it is operated for a period of 5 minutes. The germination percentage was improved from 77 % to 95 % with  1000 seed weight of 73.21 mg, and the observed recovery was 56 per cent with the vigour index of 1145. Hence, BSS 22 X 22 retained mesh sieve with a duration of 5 minutes could be recommended as an optimum sieve size for grading Amaranthus seeds for improving the seed quality

A RAPID ASSAY METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF EPLERENONE IN TABLETS BY UV SPECTROPHOTOMETRY

Objective: To develop a rapid, simple and a cheap UV spectrophotometric assay method for the estimation of Eplerenone in tablets and validate as per ICH guidelines.Methods: The optimized method uses 0.05N HCl as a solvent for the estimation of assay of Eplerenone in tablets at a detection wavelength of 245 nm.Results: System precision and intra-day precision are exemplified by relative standard deviation of 1.5% and 1.3% respectively. Method was found to be rugged as precision was found to be 1.2%. The developed method resulted in Eplerenone exhibiting linearity in the range 5-15μg/ml. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. Conclusion: A rapid, simple and a cheap UV spectrophotometric assay method was developed and validated for the estimation of Eplerenone in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â

Anti asthmatic effect of Momordica Charantia and its comparison with montelukast an in vitro and in vivo model

Background: Momordica charantia (MC) (bitter gourd) have shown the inhibition of NF-κβ and Leukotrienes expression in many inflammatory pathological conditions. Based on ist anti-inflammatory action this study aimed to Identify MC fruit dry powder (MCp) and MC fresh juice (MCj) action on airway inflammation in Guinea Pig model and also to find out the presence of alkaloids and flavonoides.Methods: 18 adult Guinea pigs of both sexes (excluding mating animals) were randomly divided into three groups with six animals in each namely Montelukast, MCj and MCp groups respectively. They were given with OD oral administration of concerned drugs for 1 -7 days. 6 control animals were exposed to 2% histamine aerosol for 120 seconds using histamine chamber prior to treatment with Montelukast, MCj and MCp to observe the normal Pre-Convulsive Dyspnea (PCD) in seconds. After six hours of daily OD oral dose of test drugs administration, all the groups’ were exposed to 2% Histamine aerosol one by one for 120 sec on day1 & 7 to observe PCD. The MCj and MCp were also tested with chemical assay, TLC to confirm the presence of alkaloids, flavonoides.Results: ON DAY 1 and 7: MCj and MCp showed significant decrease in PCD occurrence and it is statistically significant compared to the control. PCD blocking action of MCj group was effective in DAY 1. TLC and Chemical Assay were not supportive for bronchodilator action.Conclusions: MCj and MCp have promising preventive role in asthma. This study had expressed the positive protective role of Momordica charantia in asthmatic condition based on its known anti-inflammatory action.

An Ayurvedic approach to the management of Shushkakshipaka w.s.r. to Dry Eye Syndrome - A Case Report

Introduction: Sushkakshipaka is one among the Sarvagata, Sadhya Netrarogas described in in classical Ayurveda texts. A feature of Shushkakshipaka includes hard and dry eye lids, found drooping; blurred vision and difficulty to open the eyes with irritation and foreign body sensation. The Lakshanas of Shushkakshipaka can be equated with the signs and symptoms of Dry Eye Syndrome. Dry eye is a multifactorial ocular surface disease characterized by symptoms of discomfort, irritation, and visual disturbance. Here the patient presenting with signs and symptoms of dry eye disease was treated according to the treatment principles of Shushkakshipaka as explained in Ayurvedic classics. Erandamoola Kheera Seka and Shatavari Ghrita was selected for the treatment and the same has been presented in the article. Materials and Methods: The subject who approached Shalakya Tantra OPD of Sri Jayachamarajendra Institute of Indian Medicine Hospital, Bengaluru, with signs and symptoms of dry eye disease was thoroughly examined and systematically reviewed. Treatment was planned based on the Chikitsasutra of Shushkakshipaka. Result: The patient showed considerable improvement subjectively and objectively. Improvement in Schirmer’s reading, visual acuity improvement was observed in both eyes. Conclusion: The maintenance of stability of tear film is the main challenge in this pandemic time due to the increased screen time. This can be achieved up to an extend by proper administration of treatment techniques described in classical Ayurveda texts along with Pathyasevana

Role of Protein Flexibility in Ion Permeation: A Case Study in Gramicidin A

AbstractProteins have a flexible structure, and their atoms exhibit considerable fluctuations under normal operating conditions. However, apart from some enzyme reactions involving ligand binding, our understanding of the role of flexibility in protein function remains mostly incomplete. Here we investigate this question in the realm of membrane proteins that form ion channels. Specifically, we consider ion permeation in the gramicidin A channel, and study how the energetics of ion conduction changes as the channel structure is progressively changed from completely flexible to a fixed one. For each channel structure, the potential of mean force for a permeating potassium ion is determined from molecular dynamics (MD) simulations. Using the same molecular dynamics data for completely flexible gramicidin A, we also calculate the average densities and fluctuations of the peptide atoms and investigate the correlations between these fluctuations and the motion of a permeating ion. Our results show conclusively that peptide flexibility plays an important role in ion permeation in the gramicidin A channel, thus providing another reason—besides the well-known problem with the description of single file pore water—why this channel cannot be modeled using continuum electrostatics with a fixed structure. The new method developed here for studying the role of protein flexibility on its function clarifies the contributions of the fluctuations to energy and entropy, and places limits on the level of detail required in a coarse-grained model