Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography

Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions. Here we describe a simple method that allows rapid determination of the most abundant soluble sugars (glucose, fructose and sucrose) in Arabidopsis leaves by anion exchange chromatography. We have applied this method to study the levels of soluble sugars during the photoperiodic transition to flowering (Ortiz-Marchena et al., 2014).España, MINECO projects CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00España, Junta de Andalucía P06-CVI-01450 and P08-AGR-0358

Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity

Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).España,MINECO CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00España, Junta de Andalucía P06-CVI-01450 and P08-AGR-0358

Capas de Tormentas (tempestitas) en el Jurásico del Subbético Externo (Cordilleras Béticas)

Se estudian niveles de tempestitas calcáreas en el Dogger y Malm del Subbético Externo. Se describen las secuencias de estructuras internas y se interpreta su génesis en relación con tormentas tropicales o huracanes, lo que concuerda con la paleogeografía y paleolatitud admitida para el Jurásico

Spanish albacore (Thunnus alalunga) surface fishery in the Northeastern Atlantic, summary description in 2018.

Main features of the Spanish albacore (Thunnus alalunga) surface fishery in 2018 were summarized. Fishing activity took place during summer from June to August operating in offshore waters of the northeast Atlantic and in the Bay of Biscay. Albacore was targeted by troll fleet from June to August, mostly in off shore waters of northeast Atlantic and the baitboat in the Bay of Biscay. In 2018, the catch of the baitboat fleet increased 38% while troll fleet increased 15% compared to catch in year 2017. Size composition of catches taken by baitboats and troll fleets in 2018, showed a high proportion of age 2 group, followed by equivalent proportion of age 1 and age 3 groups albacore. Monthly spatial distribution of nominal catch rates were presented for both fleets

Mealiness assessment in apples using MRI techniques

Small samples of Top Red apples stored 6 months under controlled atmosphere (expected to be non-mealy) and 2°C (expected to be mealy) have been used for MRI imaging. Multi-slice-multi-echo magnetic resonance images (64*64 pixels) have been recorded with a 8 ms echo time. Three out of four apples corresponding to the sample maintained under controlled atmosphere did not developed mealiness while three out of four fruits corresponding to the sample stored at 2°C became mealy after 6 months of storage. The minimum T2 values obtained for the mealy apples show to be significantly lower (F = 13.21) when compared with non-mealy apples pointing that a more desegregated structure and a lower juiciness content leads to lower T2 signal. Also, there is a significant linear correlation (r = −0.76) between the number of pixels with a T2 value below 35 ms within a fruit image and the deformation parameter registered during the Magness–Taylor firmness test. Finally, all T2 maps of mealy apples show a regional variation of contrast which is not shown for non-mealy apples. Significant differences (F = 19.43) between mealy and non-mealy apples are found in the histograms of the T2 maps as mealy apples show a skew histogram combined with a “tail” in their high T2 extreme which is not shown in the histograms of non-mealy apples. These histogram features are also shown for an apple showing internal breakdown indicating that in mealy apples there is a differential water movement that may precede internal breakdown