Short- and long-term effect of sulphite on sucrose transport in grapevine (Vitis vinifera L.) leaves. An electrophysiological study

Sucrose is the main carbohydrate translocated in grapevine and its transport may be restricted or inhibited by a number of factors such as the pollutant sulphur dioxide. The present study investigated, for the first time in grapevine, the effects of sulphite on membrane electrical response of sucrose transport in the mesophyll cells. Co-transport of sucrose across the membrane is linked to the free energy in a electrochemical proton gradient. Without the pollutant, electrophysiological traces displayed a metabolic-dependent sucrose electrical response in which an initial depolarization was followed by complete repolarization. In the presence of sulphite, instead, there were different trends depending on time of contact with the tissue of the pollutant. In the short-term, a slower repolarization was observed and in the long-term (after 6 and 12 h) the extent of depolarization (Δ mV) was also reduced. Transmembrane electrical potentials, measured in the presence of sulphite, became significantly (P < 0.01) more positive with increasing time of incubation of the tissue. On the whole, electrophysiological results highlight a direct or indirect effect of the pollutant on the activity of proton pump H+/ATP ase. Since carbohydrate translocation has a central role in the balance between source and sinks in the plant, the results of the research suggest that sulphite can modify the above balance with negative implications for the export of carbohydrate from the leaves

Propagative material of grapevine: RFID technology for supporting traceability of "basic" and "certified" material along the wine production chain

Four main categories of propagative material in the European Union (EU) have been indentified for grapevine: the primary source, pre-basic, basic and certified material. Each type of material has to be periodically assayed for pathogens and each material stage is intrinsically characterized by increasing risks. Radiofrequency (RFID) can be considered an efficient and durable traceability system to provide retrieval of propagated material or check producer identity. RFID tagging of grapevines of different categories along the production line could establish a durable link between stakeholder and products. To evaluate this approach, histological observations and growth parameters of basic or certified RFID- tagged grapevine were performed, as well as requirement analysis for management of sanitary checks and for traceability of the wine production chain. Basic material can be safety tagged with RFID tags to establish mother plant vineyards; derived certified material can also safely be tagged. No detrimental performance in terms of vegetative growth and bud production were reported for mother plant vineyards from the first year of production life. Requirement analysis made it possible to individuate traceability objectives, materials and stakeholders involved, as well as the RFID-tagging steps and methods to collect sanitary and production data that are useful for traceability purposes

Propagative material of grapevine: RFID technology for supporting traceability of "basic" and "certified" material along the wine production chain

Four main categories of propagative material in the European Union (EU) have been indentified for grapevine: the primary source, pre-basic, basic and certified material. Each type of material has to be periodically assayed for pathogens and each material stage is intrinsically characterized by increasing risks. Radiofrequency (RFID) can be considered an efficient and durable traceability system to provide retrieval of propagated material or check producer identity. RFID tagging of grapevines of different categories along the production line could establish a durable link between stakeholder and products. To evaluate this approach, histological observations and growth parameters of basic or certified RFID- tagged grapevine were performed, as well as requirement analysis for management of sanitary checks and for traceability of the wine production chain. Basic material can be safety tagged with RFID tags to establish mother plant vineyards; derived certified material can also safely be tagged. No detrimental performance in terms of vegetative growth and bud production were reported for mother plant vineyards from the first year of production life. Requirement analysis made it possible to individuate traceability objectives, materials and stakeholders involved, as well as the RFID-tagging steps and methods to collect sanitary and production data that are useful for traceability purposes

La tecnologia delle radiofrequenze (RFId) nella filiera vivaistico-viticola: una prima sperimentazione per la tracciabilità nel settore

Organic Eprint

RFID in viticoltura: un database online per la gestione delle piante

La banca dati sperimentale allestita dall'Associazione toscana costitutori viticoli in collaborazione con il Dipartimento di coltivazione e difesa delle specie legnose dell'Università di Pis