Guide pour l'établissement des plans de prévention et de sécurité des entreprises extérieures: Travaux et prestations de catégorie 2

Ce document a été élaboré par un groupe de travail ST/DI et TIS/GS en collaboration avec un préventeur / consultant de l'APAVE. Il est destiné à servir de guide et de référence pour l'élaboration des plans de prévention au sein de la division ST

Dinamiche post-colturali in un ceduo misto di castagno ai suoi limiti ecologici

Abstract: Until the beginning of the last century, chestnut has played an important role as staple food and primary wood source. In many cases it was cultivated at the border of its ecological limits where it was planted by man in place of the original and more site-adapted tree species. However, with the abandonment of the rural activities, management of chestnut forests was progressively left starting from more marginal areas, usually occupied by coppice stands. After the interruption of the traditional coppice management system (usual rotation periods of 10-25 years), natural intra- and interspecific competition dynamics have become the driving force of the stand evolution. This may lead to dramatic changes in both structure and species composition of the stands. The aim of this study is to analyse the post-cultural evolution of an abandoned chestnut coppice in the Pesio Valley (Piedmont, Italy) in order to highlight the competition among different "basic silvicultural components" of the forest using a dendroecological approach. The "basic silvicultural components" are intended as the elements defined as groups of trees of the stand that have similar features such as silviculturally relevant attributes: species (chestnut, beech, fir), origin (seed, sprout) and cultural age and function (standard/reserve, maiden, shoot, regeneration, dead tree). The mean growth curves of the components show the different fitness of each category. From a general point of view, the beech and fir components show a better competitive potential in comparison with chestnut. Among chestnut components, maidens from seeds reveal a better growth trend compared to coppice shoots and standards

Prediction of self-assembly of adenosine analogues in solution: a computational approach validated by isothermal titration calorimetry.

The recent discovery of the role of adenosine-analogues as neuroprotectants and cognitive enhancers has sparked interest in these molecules as new therapeutic drugs. Understanding the behavior of these molecules in solution and predicting their ability to self-assemble will accelerate new discoveries. We propose a computational approach based on density functional theory, a polarizable continuum solvation description of the aqueous environment, and an efficient search procedure to probe the potential energy surface, to determine the structure and thermodynamic stability of molecular clusters of adenosine analogues in solution, using caffeine as a model. The method was validated as a tool for the prediction of the impact of small structural variations on self-assembly using paraxanthine. The computational results were supported by isothermal titration calorimetry experiments. The thermodynamic parameters enabled the quantification of the actual percentage of dimer present in solution as a function of concentration. The data suggest that both caffeine and paraxanthine are present at concentrations comparable to the ones found in biological samples

Biomimetic magnetic sensor for electrochemical determination of scombrotoxin in fish

This work addresses a novel, rapid and cost-effective approach for the electrochemical sensing of scombrotoxin (histamine) in fish based on magnetic molecularly imprinted polymer (magnetic-MIP). The histamine magnetic-MIP was synthesized by the core-shell method using histamine as a template, and 2-vinyl pyridine as functional monomer. The magnetic-MIP was characterized by TEM, SEM, and confocal microscopy. Additionally, the binding capacity of magnetic-MIP towards histamine was investigated and compared with magnetic non-molecularly imprinted polymer (magnetic-NIP). This biomimetic material merged the advantages of MIPs and magnetic particles (MPs), including low cost of production, stability, high binding capacity and can be easily separated by the aid of a permanent magnet. The magnetic-MIP was integrated into magneto-actuated electrodes for the direct electrochemical detection of histamine preconcentrated from fish samples. The results revealed that this approach succeeded in the preconcentration and determination of histamine with a LOD as low as 1.6 × 10−6 mg L−1, much lower than the index for fish spoilage (50 mg kg−1) accordingly to the legislation. Furthermore, the analytical performance was validated for the determination of histamine in scombroid fish samples with recovery values ranging from 96.8 to 102.0 %, confirm so it can be applied easily for routine food examination.Fil: Hassan, Amal H. A.. Universitat Autònoma de Barcelona; España. Beni suef University; EgiptoFil: Sappia, Luciano David. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Moura, Silio Lima. Universitat Autònoma de Barcelona; EspañaFil: Ali, Fatma H. M.. Beni Suef University; EgiptoFil: Moselhy, Walaa A.. Beni Suef University; EgiptoFil: Sotomayor, María del Pilar Taboada. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia.; BrasilFil: Pividori, María Isabel. Universitat Autònoma de Barcelona; Españ

Boschi di neoformazione in Italia: approfondimenti conoscitivi e orientamenti gestionali

Nelle regioni meridionali, e in Sicilia in particolare, la fisionomia della vegetazione forestale post-abbandono è quella della macchia o arbusteto che difficilmente evolve verso un bosco propriamente detto. Il processo evolutivo della vegetazione spesso non raggiunge lo stadio di bosco non perché le condizioni ambientali non lo consentano ma per due ragioni fondamentali: i disturbi, in particolare gli incendi e il pascolo, e le limitazioni nell’arrivo di propaguli, causate dalla mancanza di piante madri, dei dispersori dei semi o entrambi. Infatti, in contesti favorevoli (assenza di disturbi e arrivo dei propaguli) la vegetazione evolve sino al bosco. Le formazioni preforestali frutto dei processi di successione secondaria occupano superfici significative e sono conseguenza del fenomeno dell’abbandono dell’agricoltura iniziato alla seconda metà del secolo scorso e che ha interessato tutta l’Europa. Alcune azioni per ridurre i fattori negativi ed esaltare invece i vantaggi ambientali sono possibili per i boschi degli ambienti mediterranei. Tra queste, la trasformazione dei boschi di neoformazione in sistemi agroforestali, e più propriamente silvopastorali, utilizzando la parte aerea delle specie arboree e arbustive e mantenendo piccoli nuclei di specie spontanee utili alla fauna selvatica (a esempio, specie con frutti carnosi) senza lasciare che esse dominino la vegetazione erbacea. Ciò consentirebbe una valorizzazione delle superfici innanzitutto come pascoli, ma non si esclude l’utilizzazione come legna da ardere della componente arborea e, in certi casi, dei prodotti ottenibili (a esempio, manna, mandorle, carrube, nocciole). Altra possibilità per non disperdere i vantaggi consisterebbe nel mettere a coltura gli ex coltivi adottando tecniche alternative che non disperdano il carbonio accumulato. Su questo aspetto esistono già esperienze: ciò potrebbe inoltre ridurre la conflittualità tra gli enti gestori delle aree protette, restii a consentire un ritorno alla coltivazione, e gli agricoltori nonché i sostenitori della necessità di tutelare i paesaggi agrari

Exploiting the GTEx resources to decipher the mechanisms at GWAS loci.

The resources generated by the GTEx consortium offer unprecedented opportunities to advance our understanding of the biology of human diseases. Here, we present an in-depth examination of the phenotypic consequences of transcriptome regulation and a blueprint for the functional interpretation of genome-wide association study-discovered loci. Across a broad set of complex traits and diseases, we demonstrate widespread dose-dependent effects of RNA expression and splicing. We develop a data-driven framework to benchmark methods that prioritize causal genes and find no single approach outperforms the combination of multiple approaches. Using colocalization and association approaches that take into account the observed allelic heterogeneity of gene expression, we propose potential target genes for 47% (2519 out of 5385) of the GWAS loci examined