Frozen mitochondria as rapid water quality bioassay

A rapid and relatively low cost bioassay, usable in routine screening water test has been developed modifying the beef heart mitochondria test. In our experiments, mitochondria (FM22) were frozen at 22 °C, instead of 80 °C (FM80), and their applicability and sensitivity was verified. The oxygen consumption was measured by a Clark elec- trode that was interfaced to a PC to collect test analysis data. Blank tests were carried out to verify the oxygen con- sumption linear fitting. Toxicity tests were performed using pure organic and inorganic compounds, such to verify the FM22 sensitivity. A piecewise regression, through an Excelâ Macro, identified the break-point in the oxygen con- sumption and calculated the toxicity. The IC50s of the tested compounds were calculated and ranged from 0.123 to 0.173 mg/l for heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) and from 0.572 to 10.545 mg/l for organics (benzene, DMSO, DDE, endrin, dichloromethane, chlorobenzene, 1,2-dichlorobenzene and 1,3-dichlorobenzene). Water effluent samples were then tested. The FM22 gave different toxic reactions to them. Water samples were characterised for heavy metals. The FM22 bioassay had a higher sensitivity than the FM80 and a high reproducibility in the toxicity test with pure compounds. The FM22 test was a good predictor of toxicity for water samples; the bioassay is easy, low cost and rapid, then usable for routine tests

Setaria tundra, an emerging filarioid nematode in roe deer (Capreolus capreolus) in northeast Italy.

Coincidental with decades of warming (high temperature and humidity) in the sub-Arctic region of Europe, the mosquito-borne filarioid nematode Setaria tundra is now associated with emerging epidemic disease, resulting in morbidity and mortality for reindeer and moose. Similarly, roe deer is also target of this nematode, as documented in Denmark, Germany and Bulgary. In the early 2000s, S. tundra was first described in roe deer in Piedmont (north Italy). Aedes spp. mosquitoes seem to be the most competent vectors for S. tundra and little is known about its pathogenicity in roe deer. After a first report in 2013 of S. tundra in Friuli Venezia Giulia-FVG (northeast Italy) roe deers, during 2014-2016 an investigation was undertaken to evaluate the its presence in road-killed roe deer from the Gorizia province. Currently, parasitological dissections of 17 roe deer were performed. During the necropsy, nematode specimens (1 to 58 per host) were found in the abdominal cavity. Based on their morphology and biometrics, the nematodes recovered were identified as Setaria tundra and preserved in alcohol or cryopreserved for further analysis Moreover, pathological lesions were described, documented and damaged tissue samples were collected for histological evaluation. The prevalence of S. tundra in FVG roe deer population is 47.1%, mean intensity 11 (range 1-58) and mean abundance 5. The adult worms were located free in the abdominal cavity where tracks left by worm migration were also seen. Macroscopical lesions were polysierositis with thickening spleen and liver serosa with fibrinous deposition on surface of the abdominal organs. Whereas, histological lesions were diffuse serositis, granulomatous reactivity with Muller's giant cells and calcifications. In our study in FVG, this the first recognition of S. tundra and the alarming aspect has been that some road killed roe deers showed some significant pathological peritoneal changes

Trialkyllead compounds induce the opening of the MPT pore in rat liver mitochondria

The interactions of the tributyl, triethyl and trimethyllead compounds with energized mitochondria have been investigated in this paper. It has been shown that the (alkyl)3Pb–Cl compounds induce swelling in mitochondria suspended in a sucrose medium. The phe- nomenon is more marked the higher the lipophilicity and occurs in the following order: (Bu)3Pb > (Et)3Pb > (Me)3Pb. As swelling is inhibited by cyclosporine, this suggests that the swelling is due to the opening of a trans-membrane pore (MTP pore) in the mitochondria. As this pore can be responsible for the inhibition of the ATP synthesis, and, consequently for cell death, the opening of the pore could be one of the reasons for the toxicity of the (alkyl)3Pb–X compounds

An in vitro study of the interaction of Sea-Nine with rat lever mitochondria

The interactions of the antifouling compound Sea-Ninetwith rat liver mitochondria have been studied. The results indicate that low doses of this compound inhibit adenosine 59-triphosphate (ATP) synthesis. Further investigations indicate that ATP synthesis inhibition should be due to an interaction of Sea-Nine with the succinic dehydrogenase in the mitochondrial respiratory chain

Methylmercury induces the opening of the permeability transition pore in rat liver mithocondria.

Interactions of methylmercury (CH3HgCl) with non-energized mitochondria from rat liver (non-respiring mitochondria) have been investigated in this paper. It has been shown that CH3HgCl induces swelling in mitochondria suspended in a sucrose medium. Swelling has also been induced by detergent compounds and by phenylarsine, a chemical compound which induces opening of the permeant transition pore (MTP). Opening of the MTP is inhibited by means of cyclosporine A. Results indicate that the swelling induced by CH3HgCl, as in the case of phenylarsine, is inhibited by cyclosporine A and Mg , while swelling induced by detergent compounds is not cyclosporine sensitive. This comparison suggests that CH3HgCl induces opening of a permeability transition pore (MTP). Since the opening of an MTP induces cell death, this interaction with MTP could be one of the causes of toxicity of CH3HgCl

Frozen mitochondria as rapid water quality bioassay

A rapid and relatively low cost bioassay, usable in routine screening water test has been developed modifying the beef heart mitochondria test. In our experiments, mitochondria (FM22) were frozen at 22 °C, instead of 80 °C (FM80), and their applicability and sensitivity was verified. The oxygen consumption was measured by a Clark elec- trode that was interfaced to a PC to collect test analysis data. Blank tests were carried out to verify the oxygen con- sumption linear fitting. Toxicity tests were performed using pure organic and inorganic compounds, such to verify the FM22 sensitivity. A piecewise regression, through an Excelâ Macro, identified the break-point in the oxygen con- sumption and calculated the toxicity. The IC50s of the tested compounds were calculated and ranged from 0.123 to 0.173 mg/l for heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) and from 0.572 to 10.545 mg/l for organics (benzene, DMSO, DDE, endrin, dichloromethane, chlorobenzene, 1,2-dichlorobenzene and 1,3-dichlorobenzene). Water effluent samples were then tested. The FM22 gave different toxic reactions to them. Water samples were characterised for heavy metals. The FM22 bioassay had a higher sensitivity than the FM80 and a high reproducibility in the toxicity test with pure compounds. The FM22 test was a good predictor of toxicity for water samples; the bioassay is easy, low cost and rapid, then usable for routine tests

Implicações da covid-19 no ordenamento jurídico brasileiro

Divulgação dos SUMÁRIOS das obras recentemente incorporadas ao acervo da Biblioteca Ministro Oscar Saraiva do STJ. Em respeito à Lei de Direitos Autorais, não disponibilizamos a obra na íntegra.Localização na estante: 34:578.834 M149iOrganizado por: Carlos Henrique Machado, Denise M. Nunes e Ruben Rockenbach Manent

Propylthiouracil-induced cutaneous vasculitis

The use of propylthiouracil (PTU) is associated with the development of different auto-antibodies, amongst them are antineutrophil cytoplasmic antibodies (ANCA) that are involved in the pathogenesis of ANCA associated systemic vasculitis. The case of a 46-years old woman who presented cutaneous vasculitis when taking PTU for Graves' disease is reported. Perinuclear ANCA (p-ANCA) was positive with titer > 1/320, but anti-myeloperoxidase antibodies were not detected. Skin biopsy showed leucocytoclastic vasculitis. The patient improved within ten days after withdrawing PTU and the resolution of hyperthyroidism was achieved with radioiodine (131I). The p-ANCA test remained positive > 1/320 eight months and four years after PTU withdrawal.O uso do propiltiouracil (PTU) está associado ao desenvolvimento de diferentes auto-anticorpos, entre eles anticorpos anticitoplasma de neutrófilos (ANCA), que estão envolvidos na patogênese das vasculites sistêmicas ANCA-associadas (VSAA). Será relatado o caso de um paciente do sexo feminino, de 46 anos, que apresentou vasculite cutânea durante o uso de PTU como terapêutica para doença de Graves. O ANCA com padrão perinuclear (p-ANCA) foi positivo em títulos > 1/320, porém anticorpos antimielo-peroxidase (MPO) não foram detectados. A biópsia de pele revelou uma vasculite leucocitoclástica. Houve melhora clínica em dez dias após a retirada do PTU e optou-se pelo iodo radioativo (I131) para o tratamento do hipertireoidismo. O p-ANCA manteve-se positivo em títulos > 1/320 em duas medidas, realizadas oito meses e quatro anos após a suspensão do PTU.Hospital do Servidor Público Estadual Francisco Morato de OliveiraUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaUNIFESP, EPMSciEL

REGULUS CubeSat Propulsion System: In-Orbit Operations

A robust, versatile, and cost-effective propulsion system to provide wide mobility to small satellite platforms and nanosatellite deployers. A Plug&Play propulsion system designed to be easily integrated into different satellite platforms and to match customer\u27s requirements, with minimal customization efforts and costs