421 research outputs found

    Comparison of cloud boundaries measured with 8.6 mm radar and 10.6 micrometer lidar

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    One of the most basic cloud properties is location; the height of cloud base and the height of cloud top. The glossary of meteorology defines cloud base (top) as follows: 'For a given cloud or cloud layer, that lowest (highest) level in the atmosphere at which the air contains a perceptible quantity of cloud particles.' Our studies show that for a 8.66 mm radar, and a 10.6 micrometer lidar, the level at which cloud hydrometers become 'perceptible' can vary significantly as a function of the different wavelengths, powers, beamwidths and sampling rates of the two remote sensors

    Diversity assessment of seedlings from self-pollinated Sangiovese grapevines by ampelography and microsatellite DNA analysis

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    A population of Vitis vinifera L. seedlings deriving from a single self-pollinated Sangiovese vine were assayed for diversity by ampelographic and genetic techniques. After field-transplantation in 1987, the seedlings were initially screened in 1995. Twenty-four seedlings were of standard Vigour and grape production. Woody cuttings from the 24 seedlings and the mother plant were self-rooted in 1995, and each Vine was morphologically analysed and compared in 1997 using 31 descriptors of the ampelographic data sheet (OIV 1983) which are also recommended by UPOV for varietal identification - three for young shoots, 7 for shoots at bloom, 17 for adult leaves, one for flowers and 3 for berries. - In 1996 DNA was extracted from young apical leaves of the mother plant and the 24 seedlings. Ten molecular microsatellites, VVS1, VVS2, VVS5, VVS16, VVS29, VVMD5, VVMD6, VVMD7, VVMD17 and VVMD28, were used for progeny and mother plant comparison. The descriptor-based analysis showed that 12 of the 24 seedlings were morphologically similar amongst themselves and not different fr-om the mother; the remaining 12 differing from each other and from the parent. The microsatellite analysis differentiated all 24 seedlings from the mother plant. Only two seedlings showed the same allele patterns at the 10 tested loci, although they differed morphologically. The results of both analyses indicate that self-pollination can generate phenotypically similar individuals that are difficult to distinguish morphologically, while their genetic polymorphism can easily be detected by microsatellite analysis. Thus it is possible, as suggested by RIVES (1961), that certain ancient cultivars comprise a number of clones that derive via vegetative propagation from closely related mother plants. Corroboration of the polyclonal origin in such cases can be performed by techniques combining morphological and molecular approaches

    Molecular characterization of officially registered Sangiovese clones and of other Sangiovese-like biotypes in Tuscany, Corsica and Emilia-Romagna

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    The present study was designed (1) to identify and determine the origin of the genetic variability via SSR and AFLP within a group of 39 Sangiovese clones officially listed in the National Grapevine Registry, (2) to pinpoint varietal differences and potential family relations among 34 Sangiovese-like biotypes, via the SSR markers. Most biotypes are regarded as Sangiovese but sometimes are known under different names. In both studies the reference standard was the registered Sangiovese clone SG 12T.No polymorphism was found among the officially listed 39 clones analysed at 6 microsatellite loci. This enabled us to confirm their origin from a single mother plant, thereby supporting the view that any morphological or qualitative differences may be the result of propagation-related mutagenic events. A subsequent AFLP analysis of 26 of the 39 clones showed polymorphic bands in three of them (two identical) that may correspond to a mutagenic event. Assays with SSR markers on 34 Sangiovese-like biotypes collected in Tuscany, Corsica and Emilia-Romagna showed that 28 are identical with the reference Sangiovese clone SG 12T, while the remaining 6 (Sangiovese 1, Sangiovese 6, Morellino, Poverina, Sangiovese forte and Brunellone) are genetically different from SG 12T and among one another so that no direct family relations could be established

    Vine performance and leaf physiology of conventionally and minimally pruned Sangiovese grapevines

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    A three-year-study (1996-1998) was carried out on field-grown Sangiovese grapevines under the temperate, humid climate of the Po Valley (northern Italy) to assess their degree of adaptation to minimal pruning (NIT) as compared to conventional pruning (CP). Evaluation included canopy growth, yield, berry ripening and grape rot incidence. In 1996 leaf function was determined as net assimilation (A); seasonal total canopy light interception and percentage of canopy gaps were also measured. As compared to CP, over the 3-year period MP showed typical features of a more rapid canopy development and earlier growth cessation, higher shoot number with shorter shoots and smaller leaves, higher yields with smaller and looser clusters less susceptible to bunch rot. Must sugar concentration was significantly lowered. Seasonal rates of A recorded on single leaves of the two pruning treatments at different shoot positions were overall similar except for higher A in young MT leaves having a chronological age of about 8-10 d. MP also showed the tendency of retarded leaf senescence. The seasonal total light interception trends indicated a linear increase with formation of leaf area in CP, whereas total fight interception in MT augmented until about 50 % of canopy filling and then remained constant with further leaf area development. This suggests that about half the leaf area produced in the MP vines contributed mostly to mutual shading rather than enhancing light interception capability, and, hence, photosynthetic capacity. The overall data indicate that a satisfactory balance in the minimally pruned Sangiovese grapevines can only be reached with further adjustments of crop level to be performed either as partial winter and/or summer pruning. Nevertheless, in MP vines the consistent feature of looser clusters which are much less susceptible to bunch rot is crucial in an area usually characterized by abundant rainfall at pre-harvest.

    Synthesis in mesoreactors: Ru(porphyrin)CO-catalyzed aziridination of olefins under continuous flow conditions

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    The Ru(porphyrin)CO-catalyzed addition of aryl azides to styrenes to afford N-aryl aziridines was successfully performed for the first time in mesoreactors under continuous flow conditions

    Site-fidelity and movement patterns of bottlenose dolphins (<i>Tursiops truncatus</i>) in central Argentina: essential information for effective conservation

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    The effectiveness of conservation measures such as marine protected areas (MPAs) for the conservation of cetaceans is determined by how well their home range or critical habitat is covered. The present study seeks to provide information on the site-fidelity and movement patterns of individual bottlenose dolphins (Tursiops truncatus) in central Argentina. Between 2007 and 2013, photo-identification data of bottlenose dolphins were collected in four study sites some 90-200km apart from each other along the central Argentinean coast. Results show long-term site-fidelity (over 5years) in one of the study areas. Re-sighting rates further suggest the existence of different sub-populations of bottlenose dolphins, but also confirm some connectivity (with movements over 200-290km) and thus potential for gene flow within the region. Considering the population declines of bottlenose dolphins in Argentina, information on site-fidelity and movement patterns will be of value to improve the effectiveness of existing MPAs for the conservation of the species as well as prioritizing areas for increased research

    Determination of n-3 index and arachidonic acid/eicosapentaenoic acid ratio in dried blood spot by gas chromatography

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    Background: Clinical and epidemiological studies suggest that analysis of the polyunsaturated fatty acids (PUFAs) is essential to evaluate nutritional requirements and disease risk. We describe a simple, sensitive and non-invasive method for estimating the n-3 index and arachidonic acid (AA)/eicosapentaenoic acid (EPA) ratio in dried blood spots (DBSs). Experimental: After obtaining DBSs on a spot card, PUFAs were transesterified (direct, acidic transesterification) and subsequently extracted with n-hexane. Gas chromatography with flame ionization detection (GC-FID) was used to analyze the extracted PUFAs, and then n-3 index and AA/EPA ratio were calculated. Method validation showed satisfactory precision and linearity. Conclusion: This analysis is simple and reliable to estimate PUFA status, and it was successfully applied to samples from 20 subjects, demonstrating its applicability

    Sinkhole monitoring and early warning: An experimental and successful GB-InSAR application

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    AbstractSinkholes represent a natural risk that may hit catastrophically without clearly detectible precursors. However, they are often overlooked by people and administrators. Therefore sinkhole monitoring and associated early warnings constitute important research topics but, currently, only a few papers about sinkhole prediction can be found. In this paper an experience of sinkhole monitoring and early warning with GB-InSAR is described. The latter is a highly precise instrument that is able to produce displacement maps with metric spatial resolution. The described activities were carried out on Elba Island (central Italy), where karstified limestone set off the occurrence of nine sinkholes since 2008, all within less than 3000m2, causing major damage to an important road and many indirect losses. In 1year of monitoring two deforming areas were detected, and the point where a sinkhole was about to propagate to the street level was predicted, thus permitting the preventive closure of the road. The deformation area was larger than the hole generated by the sinkhole, thus showing a subsidence that continued for a prolonged time even after the cavity was filled up. The occurrence of a 1.5-m-wide sinkhole, undetected by the GB-InSAR, also showed the lower detection limit of the instrument

    Review of ten years of volcano deformations recorded by the ground-based InSAR monitoring system at Stromboli volcano: a tool to mitigate volcano flank dynamics and intense volcanic activity

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    AbstractStromboli volcano (Southern Italy) is one of the most monitored volcano in the world with a surveillance network that includes a permanently sited ground-based SAR interferometer (GBInSAR). This work is the review of the GBInSAR data gained from the last decade of monitoring activity. The analysis of the entire dataset of GBInSAR measurements allowed the assessment of the deformation field of the northern part of the summit crater area and the Sciara del Fuoco depression. In detail, the main displacements recognized can be related to different factors: 1) the inflation/deflation respectively immediately before and after each new effusive event; 2) the bulging of localized sectors of the volcano involved in the vent opening; 3) the gravitational sliding of the Sciara del Fuoco infill; 4) the movement of lava flows. Accelerations in this sector are related to sheet intrusions, while the possibility of vent opening, associated with small sliding, or catastrophic flank failure are related to highly overpressurized sheets, able to produce high displacement rate in the Sciara del Fuoco.In the summit crater area, the increases in the displacement rate are related to the pressurization of the shallow conduit system, as the consequence of the variation in the magma level (magmastatic pressure) or to the lateral magma migration (lateral conduit expansion or dike intrusion) in response to the increase of the overpressure component. Fluctuations in the displacement rate in the summit crater area can be related to the magma overturning within the conduit, with the increases in displacement rate during the upwelling of less dense magma, while displacement rate decreases as the degassed magma column is pushed out from the conduit (lava flows or overflows). Instead, the decrease in the displacement rate without coeval lava outpouring could be related to the sink of the degassed magma due to density contrast between the gas-poor and the gas-charged magmas. Using the displacement rate in the summit crater area as a proxy for the variation in the pressure condition in conduit (both magmastatic and overpressure components), thresholds for the crises characterized by the occurrence of overflows (eventually associated with major explosions) and flank effusions (eventually associated with paroxysmal explosions) are identified. Small conduit overpressure will produce overflows (sometimes associated with crater-rim collapses), while large magma overpressure will laterally expand the conduit forming NE-SW striking sheets, feeding eruptive vents at the base of the summit crater area and within the Sciara del Fuoco, generating conditions of instability that can evolve into catastrophic collapse of the instable flank

    Remote sensing data from CLARET: A prototype CART data set

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    The data set containing radiation, meteorological , and cloud sensor observations is documented. It was prepared for use by the Department of Energy's Atmospheric Radiation Measurement (ARM) Program and other interested scientists. These data are a precursor of the types of data that ARM Cloud And Radiation Testbed (CART) sites will provide. The data are from the Cloud Lidar And Radar Exploratory Test (CLARET) conducted by the Wave Propagation Laboratory during autumn 1989 in the Denver-Boulder area of Colorado primarily for the purpose of developing new cloud-sensing techniques on cirrus. After becoming aware of the experiment, ARM scientists requested archival of subsets of the data to assist in the developing ARM program. Five CLARET cases were selected: two with cirrus, one with stratus, one with mixed-phase clouds, and one with clear skies. Satellite data from the stratus case and one cirrus case were analyzed for statistics on cloud cover and top height. The main body of the selected data are available on diskette from the Wave Propagation Laboratory or Los Alamos National Laboratory
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