İntramusküler interlökin-8 enjeksiyonunun lokal anjiyoneogenetik etkisi

Objective: Angioneogenesis, which plays significant roles in a variety of physiological processes such as embryonic growth and wound healing, is strictly delimited and is finely tuned by a balance of proangiogenic and antiangiogenic factors. This study was conducted to investigate the angioneogenic effect of interleukin-8 (IL-8) administered intramuscularly. Material and Methods: Twelve New Zealand white rabbits were included in this study. A total daily dose of 4 micrograms (1 mcg/kg) of IL-8 was administered into the left (Group A) and saline solution into the right (Group B) gluteus maximus muscles of 6 rabbits for 6 days. The remaining 6 rabbits constituted the sham group (Group C). Gluteal muscle samples were obtained from injection sites in all groups and were stained with hematoxylin-eosin and immunohistochemically by using streptavidin biotin method with CD31 antibody. Avidin-biotin peroxidase method (LSAB) was used as secondary and binding antibody. Diaminobenzidine tetrahydrochloride (DAB) was used as chromogenic substance. In immunohistochemical staining with CD31, vascular channels covered with brown stained cells or cell clusters were considered and were counted as vascular network. Results: Three subjects in Group A and one subject in Group B displayed findings of large muscle necrosis and regeneration. Vascular channel score was significantly higher in Group A (p=0.032) (Group A; median=12.5, min=6, max=16. Group B; median=5, min=4, max=13. Group C; median=4.5, min=4, max=13.) than in the other groups. Conclusion: IL-8 chemokine family seems to stimulate angioneogenesis in rabbit skeletal muscles. This result is promising in terms of the possible therapeutic potential of IL-8. Daily administration at a dose of around 1 mcg/kg caused local tissue necrosis, hence use of alternative routes such as intraarterial administration must be investigated to avoid such complications. © 2012 by Türkiye Klinikleri

Early pregnancy regulates genes of hyaluronan system in ovine endometrium

Joint Meeting of the 20th Annual Conference of the European-Society-for-Domestic-Animal-Reproduction (ESDAR) / 13th Conference of the Spanish-Association-for-Animal-Reproduction (AERA) -- OCT 27-29, 2016 -- Lisbon, PORTUGALWOS: 000392391400175European Soc Domest Anim Reprod, Spanish Assoc Anim ReprodTUBITAK grant [214O643]This study was partially funded by TUBITAK grant 214O643 to MK

Organic and inorganic mercurials have distinct effects on cellular thiols, metal homeostasis, and Fe-binding proteins in Escherichia coli

The protean chemical properties of the toxic metal mercury (Hg) have made it attractive in diverse applications since antiquity. However, growing public concern has led to an international agreement to decrease its impact on health and the environment. During a recent proteomics study of acute Hg exposure in E. coli, we also examined the effects of inorganic and organic Hg compounds on thiol- and metal- homeostases. On brief exposure, lower concentrations of divalent inorganic mercury Hg(II) blocked bulk cellular thiols and protein-associated thiols more completely than higher concentrations of monovalent organomercurials, phenylmercuric acetate (PMA) and merthiolate (MT). Cells bound Hg(II) and PMA in excess of their available thiol ligands; X-ray absorption spectroscopy indicated nitrogens as likely additional ligands. The mercurials released protein bound iron (Fe) more effectively than common organic oxidants and all disturbed the Na(+)/K(+) electrolyte balance, but none provoked efflux of six essential transition metals including Fe. PMA and MT made stable cysteine monothiol adducts in many Fe-binding proteins, but stable Hg(II) adducts were only seen in CysXxx(n)Cys peptides. We conclude that on acute exposure: (a) the distinct effects of mercurials on thiol- and Fe-homeostases reflected their different uptake and valences; (b) their similar effects on essential metal- and electrolyte-homeostases reflected the energy-dependence of these processes; and (c) peptide phenylmercury-adducts were more stable or detectable in mass spectrometry than Hg(II)-adducts. These first in vivo observations in a well-defined model organism reveal differences upon acute exposure to inorganic and organic mercurials that may underlie their distinct toxicology