Tyrosine kinase inhibitor therapy-induced changes in humoral immunity in patients with chronic myeloid leukemia

Purpose Tyrosine kinase inhibitors (TKIs) have well-characterized immunomodulatory effects on T and NK cells, but the effects on the humoral immunity are less well known. In this project, we studied TKI-induced changes in B cell-mediated immunity. Methods We collected peripheral blood (PB) and bone marrow (BM) samples from chronic myeloid leukemia (CML) patients before and during first-line imatinib (n = 20), dasatinib (n = 16), nilotinib (n = 8), and bosutinib (n = 12) treatment. Plasma immunoglobulin levels were measured, and different B cell populations in PB and BM were analyzed with flow cytometry. Results Imatinib treatment decreased plasma IgA and IgG levels, while dasatinib reduced IgM levels. At diagnosis, the proportion of patients with IgA, IgG, and IgM levels below the lower limit of normal (LLN) was 0, 11, and 6% of all CML patients, respectively, whereas at 12 months timepoint the proportions were 6% (p = 0.13), 31% (p = 0.042) and 28% (p = 0.0078). Lower initial Ig levels predisposed to the development of hypogammaglobulinemia during TKI therapy. Decreased Ig levels in imatinibtreated patients were associated with higher percentages of immature BM B cells. The patients, who had low Ig levels during the TKI therapy, had significantly more frequent minor infections during the follow-up compared with the patients with normal Ig values (33% vs. 3%, p = 0.0016). No severe infections were reported, except recurrent upper respiratory tract infections in one imatinib-treated patient, who developed severe hypogammaglobulinemia. Conclusions TKI treatment decreases plasma Ig levels, which should be measured in patients with recurrent infections.Peer reviewe

مكونات نباتات دوله قطر : الجزء 24 الفحص الكيميائي لنبات السالفيا (نعيم)

The study of the flavonoids revealed the isolation and identification of luteolin, luteolin 7-O- glucoside, apigenin and apigenin 7-0 glucoside; their identity was proved by m.p., TLC, UV, IR and MS. Moreover, a coumarin was isolated and identified as herniarin. Investigation of the lipid fraction resulted in the isolation and identification of a and P amyrins. The fatty acids were identified as undecanoic, dodecanoic, tridecanoic, myristic, pentadecanoic, palmitic, heptadecanoic, stearic, oleic and linoleic acids.أسفرت دراسة الفلافونيدات عن فصل والتعرف على المركبات الاَتية : ليتيولين ، ليتيولين - 7 - جليكوزيد ، أبيجنين ، أ بيجنين - 7 - جليكوزيد . كما اسفرت دراسة الجزء الدهني للنبات عن فصل والتعرف على ألفا، بيتا - أميرين . وتم التعرف على الأحماض الدهنية

Human Papilloma Virus (HPV) in Egyptian women: Molecular diagnosis and genotyping

No Abstract

دراسة كيميائية لنبات الثيمس ديكساتس (الزعتر) 1- الفلافونيدات و الزيوت الطيارة

Investigation of the flavonoidal constituents of Thymus decassatus, L. resulted in the isolation and identification of thymonin, 5,6,4'-trihydroxy-7,3'-dimethoxyl flavone, and apigenin. Investigation of the volatile oil by GC revealed the identification of 41 components. Moreover, thymol as the major component was isolated from the volatile oil fraction.أسفرت .دراسة الفلافونيدات لنبات الزعتر عن فصل وتعريف كل من الثيمونين 5 ، 6 ، 4 - تراى هيدروكسي -7 - 3 ، داى ميثوكسي فلافون وكذلك الابيجينين . وأظهرت دراسة الزيت الطيار بواسطة كروماتوجرافيا الغاز عن التعرف على 41 مركب متضمنة مادة الثيمول الذي تم فصلها من جزء الزيت الطيار

Isolation and Characterization of Antineoplastic Alkaloids from Catharanthus Roseus L. Don. Cultivated in Egypt

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone)