Development and Evaluation of a Semi-automatic Cucumber Seed Extractor

IntroductionAccording to FAO, gherkin and cucumber have been cultivated in about 2.23 million hectares of farmlands around the globe, and about 78000 hectares of Iran agricultural fields have been devoted to gherkin and cucumber cultivation. However, large amounts of the cucumber seeds required in Iran have been imported from abroad. Fortunately, some Iranian agricultural companies have been focused on seed production recently. Therefore, there is an opportunity to develop seed production equipment such as seed extracting machines.There are two types of cucumber seed extractors: bulk seed extractor and single fruit extractor. The bulk seed extractor is suitable for extraction of large amounts of seed on the farm, while the single fruit extractor is suitable where seed production is limited to greenhouse seed-producing facilities which are mostly used for controlled pollination of the crop.Due to the high price of imported seed extractors, especially after increasing the price of foreign currencies, production of these machines within the country is economically rational; therefore, the aim of this research is to develop and evaluate a single fruit cucumber seed extractor for small and medium-size seed-producing greenhouses.Materials and MethodsThe cucumber seed extractor was designed semi-automatically. The criteria for designing the machine were as follows: 1- usage of the single fruit seed extraction method, 2- the possibility of simultaneous usage of two operators, 3- automatic discharge of the extracted seeds, 4- the possibility of the height variation of the machine, 5- the possibility of the emergency shutdown of the machine, 6- the possibility of the angular velocity variation of the machine helix.The working element of the machine was the rotating helix that can extract the fruit seeds and leave the fruit's outer skin mostly intact for easy disposal of fruit remains from the greenhouse. A 1 hp, 3 phase electric motor was used to drive the machine via the belt and pulley transmission system. For the smooth start and stop of the machine, and the possibility of angular velocity variation of the machine helixes, an electronic driver was used for the motor.To design the machine helix, the dimensions of the examined cucumber were measured first (i.e., cucumber length and diameter); then, according to the physical and mechanical properties of the fruit, the prime mover and transmission system of the machine was designed. Finally, the fabricated machine was evaluated using some mechanization criteria.Results and DiscussionSome of the results are as follows:The total torque required to drive the machine was 3.394Nm.The electric motor power should be higher than 0.284hp; furthermore, in order to have a wider angular velocity span for the rotating shaft, a 1hp electric motor was installed on the machine.The diameter of the rotating shaft was calculated to be 15mm.The seed extracting machine could be used instead of 15 workers of the manual seed extraction method.The rate of seed extraction using the machine was 30781 kg ha-1.The efficiency of seed extraction using the machine was similar to that of the manual method.The emergence percentage of seeds obtained by the machine was similar to that of the manual method.The amount of crop calculated from the concept of economical break-even point was 7 tons.ConclusionIn this study, an apparatus for mechanized extraction of cucumber seed was designed, fabricated and evaluated. The working element of the machine is the extractor helix that is rotated via the belt and pulley transmission system, which is activated using a 1 hp, 3 phase electric motor. For smooth start and stop of the motor, as well as the possibility of angular velocity variation of the machine helix, the motor was equipped with an electronic driver. Utilizing an emergency shutdown switch, the machine can be protected from unforeseen emergency situations. After using the machine for 6 months, it was recognized that strengthening the extractor helixes was necessary. According to the results of this study, using the machine led to 15 person reduction in the labor needs of the manual cucumber seed extraction method. Moreover, the amount of crop calculated from the economical break-even point was 7.7 tons

Non-medial infectious orbital cellulitis: etiology, causative organisms, radiologic findings, management and complications

Background: Orbital cellulitis is an ophthalmic emergency, which is associated with vision-threatening adverse effects. The purpose of this study is investigating etiology, radiologic findings, management and complications of patients with non-medial orbital cellulitis. Method: A retrospective medical record and radiologic file review of patients with infectious orbital cellulitis was performed to detect all patients with non-medial orbital cellulitis who referred to Khalili hospital from 2016 to 2019. Age, sex, origin of infection, size of collection or abscess, medical or surgical management, microbiology, first and final best-corrected visual acuity, duration of admission, and complications was recorded. Patients divided into two groups; medical management and surgical management groups and all of data compared between in this groups. Results: Of ninety-six patients with infectious orbital cellulitis, 23 cases (14 male, 9 female) were included. Five patients (21.7) were managed medically and 18 patients (78.3) were managed surgically. Patients� age range was 5�70 years old. Most common location for non-medial cellulitis was superior space (66.7 in surgical and 40 in medical group; p = 0.511). In 13 cases of surgical group (72.3) were detected microorganisms. The mean ± SD of collection volume in medical group were 476.5 ± 290.93 mm3 and 2572.94 ± 1075.75 mm3 in surgical group (p < 0.001). Ten patients in surgical group had compressive optic neuropathy. The mean ± SD of collection volume was 3204.97 ± 879.88 mm3 in patient with compressive optic neuropathy and 1280.43 ± 880.68 mm3 in patient without compressive optic neuropathy (P < 0.001). One case complicated by subdural empyema and another case progressed to necrotizing fasciitis. Conclusion: Non-medial orbital cellulitis is an uncommon but sight-threatening and life-threatening condition. Timely diagnosis and accurate management reduce morbidity and mortality. Combined surgery for patients with superior or supra-temporal and large non-medial abscess is recommended. © 2020, The Author(s)

Gold nanoprisms as a hybrid in vivo cancer theranostic platform for in situ photoacoustic imaging, angiography, and localized hyperthermia

The development of high-resolution nanosized photoacoustic contrast agents is an exciting yet challenging technological advance. Herein, antibody (breast cancer-associated antigen 1 (Brcaa1) monoclonal antibody)- and peptide (RGD)-functionalized gold nanoprisms (AuNprs) were used as a combinatorial methodology for in situ photoacoustic imaging, angiography, and localized hyperthermia using orthotopic and subcutaneous murine gastric carcinoma models. RGD-conjugated PEGylated AuNprs are available for tumor angiography, and Brcaa1 monoclonal antibody-conjugated PEGylated AuNprs are used for targeting and for in situ imaging of gastric carcinoma in orthotopic tumor models. In situ photoacoustic imaging allowed for anatomical and functional imaging at the tumor site. In vivo tumor angiography imaging showed enhancement of the photoacoustic signal in a time-dependent manner. Furthermore, photoacoustic imaging demonstrated that tumor vessels were clearly damaged after localized hyperthermia. This is the first proof-of-concept using two AuNprs probes as highly sensitive contrasts and therapeutic agents for in situ tumor detection and inhibition. These smart antibody/peptide AuNprs can be used as an efficient nanotheranostic platform for in vivo tumor detection with high sensitivity, as well as for tumor targeting therapy, which, with a single-dose injection, results in tumor size reduction and increases mice survival after localized hyperthermia treatment.National Basic Research Program of China (No. 2015CB931802)National Natural Science Foundation (China) (Nos. 81225010, 81327002, 31170961, 20771075, and 20803040)National High-Tech R&D Plan of China (No. 2014AA020700)Shanghai Science and Technology Fund (Nos. 13NM1401500 and 15DZ2252000

Thermal Imaging of Nanostructures by Quantitative Optical Phase Analysis

International audienceWe introduce an optical microscopy technique aimed at characterizing the heat generation arising from nanostructures, in a comprehensive and quantitative manner. Namely, the technique permits (i) mapping the temperature distribution around the source of heat, (ii) mapping the heat power density delivered by the source, and (iii) retrieving the absolute absorption cross section of light-absorbing structures. The technique is based on the measure of the thermal-induced refractive index variation of the medium surrounding the source of heat. The measurement is achieved using an association of a regular CCD camera along with a modified Hartmann diffraction grating. Such a simple association makes this technique straightforward to implement on any conventional microscope with its native broadband illumination conditions. We illustrate this technique on gold nanoparticles illuminated at their plasmonic resonance. The spatial resolution of this technique is diffraction limited, and temperature variations weaker than 1 K can be detected

Toxicity and cellular uptake of gold nanoparticles: what we have learned so far?

Gold nanoparticles have attracted enormous scientific and technological interest due to their ease of synthesis, chemical stability, and unique optical properties. Proof-of-concept studies demonstrate their biomedical applications in chemical sensing, biological imaging, drug delivery, and cancer treatment. Knowledge about their potential toxicity and health impact is essential before these nanomaterials can be used in real clinical settings. Furthermore, the underlying interactions of these nanomaterials with physiological fluids is a key feature of understanding their biological impact, and these interactions can perhaps be exploited to mitigate unwanted toxic effects. In this Perspective we discuss recent results that address the toxicity of gold nanoparticles both in vitro and in vivo, and we provide some experimental recommendations for future research at the interface of nanotechnology and biological systems

High Performance In Vivo Near-IR (>1 {\mu}m) Imaging and Photothermal Cancer Therapy with Carbon Nanotubes

Short single-walled carbon nanotubes (SWNTs) functionalized by PEGylated phospholipids are biologically non-toxic and long-circulating nanomaterials with intrinsic near infrared photoluminescence (NIR PL), characteristic Raman spectra, and strong optical absorbance in the near infrared (NIR). This work demonstrates the first dual application of intravenously injected SWNTs as photoluminescent agents for in vivo tumor imaging in the 1.0-1.4 {\mu}m emission region and as NIR absorbers and heaters at 808 nm for photothermal tumor elimination at the lowest injected dose (70 {\mu}g of SWNT/mouse, equivalent to 3.6 mg/kg) and laser irradiation power (0.6 W/cm2) reported to date. Ex vivo resonance Raman imaging revealed the SWNT distribution within tumors at a high spatial resolution. Complete tumor elimination was achieved for large numbers of photothermally treated mice without any toxic side effects after more than six months post-treatment. Further, side-by-side experiments were carried out to compare the performance of SWNTs and gold nanorods (AuNRs) at an injected dose of 700 {\mu}g of AuNR/mouse (equivalent to 35 mg/kg) in NIR photothermal ablation of tumors in vivo. Highly effective tumor elimination with SWNTs was achieved at 10 times lower injected doses and lower irradiation powers than for AuNRs. These results suggest there are significant benefits of utilizing the intrinsic properties of biocompatible SWNTs for combined cancer imaging and therapy.Comment: Nanoresearch, in pres

Predicting breast cancer response to neoadjuvant treatment using multi-feature MRI: results from the I-SPY 2 TRIAL.

Dynamic contrast-enhanced (DCE) MRI provides both morphological and functional information regarding breast tumor response to neoadjuvant chemotherapy (NAC). The purpose of this retrospective study is to test if prediction models combining multiple MRI features outperform models with single features. Four features were quantitatively calculated in each MRI exam: functional tumor volume, longest diameter, sphericity, and contralateral background parenchymal enhancement. Logistic regression analysis was used to study the relationship between MRI variables and pathologic complete response (pCR). Predictive performance was estimated using the area under the receiver operating characteristic curve (AUC). The full cohort was stratified by hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) status (positive or negative). A total of 384 patients (median age: 49 y/o) were included. Results showed analysis with combined features achieved higher AUCs than analysis with any feature alone. AUCs estimated for the combined versus highest AUCs among single features were 0.81 (95% confidence interval [CI]: 0.76, 0.86) versus 0.79 (95% CI: 0.73, 0.85) in the full cohort, 0.83 (95% CI: 0.77, 0.92) versus 0.73 (95% CI: 0.61, 0.84) in HR-positive/HER2-negative, 0.88 (95% CI: 0.79, 0.97) versus 0.78 (95% CI: 0.63, 0.89) in HR-positive/HER2-positive, 0.83 (95% CI not available) versus 0.75 (95% CI: 0.46, 0.81) in HR-negative/HER2-positive, and 0.82 (95% CI: 0.74, 0.91) versus 0.75 (95% CI: 0.64, 0.83) in triple negatives. Multi-feature MRI analysis improved pCR prediction over analysis of any individual feature that we examined. Additionally, the improvements in prediction were more notable when analysis was conducted according to cancer subtype