Evaluación de la calidad ambiental de ecosistemas acuáticos en el entorno del Parque Nacional de Doñana

Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 201

M3Display: Sistema de realidad aumentada para la rehabilitación de la función motora del miembro superior

Se presenta un sistema basado en realidad aumentada para la rehabilitación de la función del miembro superior en pacientes con discapacidad motora. Este sistema utiliza la tecnología de realidad aumentada para combinar elementos virtuales con el mundo real, mejorando así la experiencia del usuario. El sistema se compone de una cámara que captura el entorno real a través de la reflexión de un espejo ubicado detrás de la pantalla de visualización. A través de un algoritmo de segmentación en tiempo real, las imágenes obtenidas mediante la cámara son analizadas para detectar las manos del paciente. Posteriormente, se realiza un procesamiento adicional que permite la creación del entorno de realidad aumentada mediante la integración de diversos elementos virtuales con las manos detectadas y la imagen original capturada. Además, incorpora una interfaz de usuario que permite una interacción sencilla e intuitiva con el sistema. Esta interfaz está compuesta por seis juegos serios diseñados específicamente para la rehabilitación del miembro superior. Este sistema ofrece una solución innovadora y prometedora para la rehabilitación de la función del miembro superior en pacientes con discapacidad motora. Al combinar la tecnología de realidad aumentada con juegos serios diseñados para la rehabilitación, se brinda a los pacientes una experiencia de rehabilitación más motivadora y efectiva.Este trabajo ha sido financiado por el Ministerio de Ciencia e Innovación, a través del proyecto RTC2019-007350-1 y por la empresa TICCYL Digital S.L.U. Además, este trabajo ha sido respaldado por el Ministerio de Ciencia, Innovación y Universidades, mediante el proyecto PID2019-111023RB-C33 y por la Junta de Castilla y León por las ayudas para financiar la contratación predoctoral de personal investigador, cofinanciadas por el Fondo Social Europeo (FSE). Por ultimo, nos gustaría agradecer las ideas y sugerencias de los profesionales del Instituto de Rehabilitación Funcional (IRF) La Salle de Madrid

Omic approaches in environmental risk assessment

Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 201

Solvothermal synthesis and characterization of ytterbium/iron mixed oxide nanoparticles with potential functionalities for applications as multiplatform contrast agent in medical image techniques

A solvothermal route to prepare Glutathione capped hybrid ytterbium/iron oxide nanoparticles with potential applications as multiplatform contrast agent in medical image techniques has been developed. The influence of ytterbium/iron molar ratio used as precursor, as well as the degree of the autoclave filling on the structural and morphological characteristics of the obtained nanoparticles has been extensively studied. Although all nanoparticles present similar composition, with YbFeO3 being the majority phase, size and morphology of the as synthetized nanoparticles are highly influenced by the critical temperature and by the over -saturation reached during the solvothermal process. We have demonstrated that glutathione properly functionalizes the hybrid nanoparticles, increasing their colloidal stability and decreasing their cytotoxicity. Additionally, they show good imaging in magnetic resonance and X-ray computerized tomography, thereby indicating promising potential as a dual contrast agent. This work presents, for the first time, glutathione functionalized ytterbium/iron oxide nanoparticles with potential applications in Biomedicine. © 2022 Elsevier Ltd and Techna Group S.r.l

Clinical Role of CA125 in Worsening Heart Failure A BIOSTAT-CHF Study Subanalysis

OBJECTIVES The aim of this study was to evaluate the association between antigen carbohydrate 125 (CA125) and the risk of 1-year clinical outcomes in patients with worsening heart failure (HF).BACKGROUND CA125 is a widely available biomarker that is up-regulated in patients with acute HF and has been postulated as a useful marker of congestion and risk stratification.METHODS hi a large multicenter cohort of patients with worsening HF, either in-hospital or in the outpatient setting, the independent associations between CA125 and 1-year death and the composite of death/HF readmission (adjusted for outcome-specific prognostic risk score [BIOSTAT risk score]) were determined by using the Royston-Parmar method (N = 2356). In a sensitivity analysis, the prognostic implications of CA125 were also adjusted for a composite congestion score (CCS). Data were validated in the B1OSTAT-CHF (Biology Study to Tailored Treatment in Chronic Heart Failure validation) cohort (N = 1,630).RESULTS Surrogates of congestion, such as N-terminal pro-B-type natriuretic peptide and CCS, emerged as independent predictors of CA125. In muttivariabte survival analyses, higher CA125 was associated with an increased risk of mortality and the composite of death/HF readmission (p &lt;0.001 for both comparisons), even after adjustment for the CCS (p &lt;0.010 for both comparisons). The addition of CA125 to the B1OSTAT score led to a significant risk reclassification for both outcomes (category-free net reclassification improvement 0.137 [p &lt;0.001] and 0.104 [p 0.003] respectively). AR outcomes were confirmed in an independent validation cohort.CONCLUSIONS In patients with worsening HF, higher levels of CA125 were positively associated with parameters of congestion. Furthermore, CA125 remained independently associated with a higher risk of clinical outcomes, even beyond a predefined risk model and clinical surrogates of congestion. (C) 2020 by the American College of Cardiology Foundation.</p

Ex situ and in situ functionalized Yb/Fe nanoparticles obtained by scanning pulsed laser ablation in liquids: A route to obtain biofunctionalized multiplatform contrast agents for MRI and CT imaging

Two distinct strategies were used to improve the colloidal properties of hybrid Yb/Fe oxide NPs previously prepared by the pulsed liquid laser ablation process for use as a contrast agent in medical imaging. First, an exhaustive optimization process of the laser ablation synthesis parameters was carried out to reduce the hydrodynamic diameters of the Yb/Fe NPs. The hydrodynamic size was successfully reduced to <200 nm, thereby decreasing the polydispertivity index. Second, ex situ and in situ functionalization processes using glutathione, cysteamine, or polyethylenimine as capping agents have been developed to increase their colloidal stability at physiological pH values. Transmission electron microscopy, dynamic light scattering, Z-potential measurements, and Fourier Transform Infrared spectroscopy were used to examine the structure, morphology, colloidal and surface properties of Yb/Fe NPs. Colloidal stability of the Yb/Fe NPs as well as the linkage mechanism of functionalization have been studied extensively. This last parameter provides a critical information for subsequently bioconjugations in biomedical applications. Additionally, the biocompatibility of the Yb/Fe NPs was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) experiments. These results indicate more appropriate colloidal characteristics and higher biocompatibility for ex situ-functionalized Yb/Fe NPs, especially when the capping agent is glutathione. Additionally, these Yb/Fe NPs show good magnetic resonance imaging and X-ray computerized tomography imaging abilities, thereby indicating promising potential as dual contrast agents.This research has been funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and FEDER [research projects MAT2015-67354R], the H2020 Action H2020-MSCA-IF-2014_ST [grant 656908-NIMBLIS] of the Executive Agency for Research Manages of EU Commission, the Proyectos Integradores MdM-IMEYMAT 2020 call (research project ULST-NANO), and the projects PECART-0096-2020 (Consejería Salud y Familias. JA Spain) and P20_01293 (Consejería Economía, Conocimiento, Empresas y Universidad. JA Spain). In addition, we acknowledge the received technical assistance from the SC-ICYT of the University of Cádiz. We also acknowledge to the Networking Research Centre on Bioengineering, Biomaterials, and Nanomedicine (CIBERBBN) (which is financed by the Instituto de Salud Carlos III (ISCIII) with assistance from the European Regional Development Fund (ERDF)) and the ICTS “NANBIOSIS”, specifically the FVPR/U20 (http://www.nanbiosis.es/portfolio/u20-in-vivo-experimentalplatform/) for providing access to the micro-CT. M.L. was supported by a post-doctoral grant of the Consejo Nacional de Ciencia y Tecnología from Mexico (CONACYT, no 619639).Peer reviewe

Rationale and design of the Concordance study between FFR and iFR for the assessment of lesions in the left main coronary artery. The ILITRO-EPIC-07 Trial

Introduction and objectives: Patients with left main coronary artery (LMCA) stenosis have been excluded from the trials that support the non-inferiority of the instantaneous wave-free ratio (iFR) compared to the fractional flow reserve (FFR) in the decision-making process of coronary revascularization. This study proposes to prospectively assess the concordance between the two indices in LMCA lesions and to validate the iFR cut-off value of 0.89 for clinical use. Methods: National, prospective, and observational multicenter registry of 300 consecutive patients with intermediate lesions in the LMCA (angiographic stenosis, 25% to 60%. A pressure gudiewire study and determination of the RFF and the iFR will be performed: in the event of a negative concordant result (FFR > 0.80/iFR > 0.89), no treatment will be performed; in case of a positive concordant result (FFR 0.80/iFR 0.89), an intravascular echocardiography will be performed and revascularization will be delayed if the minimum lumen area is > 6 mm(2). The primary clinical endpoint will be a composite of cardiovascular death, LMCA lesion-related non-fatal infarction or need for revascularization of the LMCA lesion at 12 months. Conclusions: Confirm that an iFR-guided decision-making process in patients with intermediate LMCA stenosis is clinically safe and would have a significant clinical impact. Also, justify its systematic use when prescribing treatment in these potentially high-risk patients

Superficial Characteristics and Functionalization Effectiveness of Non-Toxic Glutathione-Capped Magnetic, Fluorescent, Metallic and Hybrid Nanoparticles for Biomedical Applications

An optimal design of nanoparticles suitable for biomedical applications requires proper functionalization, a key step in the synthesis of such nanoparticles, not only for subsequent crosslinking to biological targets and to avoid cytotoxicity, but also to endow these materials with colloidal stability. In this sense, a reliable characterization of the effectiveness of the functionalization process would, therefore, be crucial for subsequent bioconjugations. In this work, we have analyzed glutathione as a means to functionalize four of the most widely used nanoparticles in biomedicine, one of which is a hybrid gold-magnetic-iron-oxide nanoparticle synthetized by a simple and novel method that we propose in this article. We have analyzed the colloidal characteristics that the glutathione capping provides to the different nanoparticles and, using information on the Z-potential, we have deduced the chemical group used by glutathione to link to the nanoparticle core. We have used electron microscopy for further structural and chemical characterization of the nanoparticles. Finally, we have evaluated nanoparticle cytotoxicity, studying cell viability after incubation with different concentrations of nanoparticles, showing their suitability for biomedical applications