Nod2 Deficiency in mice is Associated with Microbiota Variation Favouring the Expansion of mucosal CD4+ LAP+ Regulatory Cells

Nucleotide-binding Oligomerization Domain-2 (NOD2) mutations are associated with an increased risk to develop Crohn's Disease. In previous studies, we have shown that Nod2-/- mice manifest increased proportion of Lamina Propria (LP) CD4+ LAP+ Foxp3- regulatory cells, when compared with Nod2+/+ mice, while CD4+ Foxp3 + regulatory cells were not affected. Here, we investigated the Nod2 gut microbiota, by 16S rRNA pyrosequencing, at steady state and after TNBS-colitis induction in mice reared separately or in cohousing, correlating the microbial profiles with LP regulatory T cells proportion and tissue cytokines content. We found that enrichment of Rikenella and Alistipes (Rikenellaceae) in Nod2-/- mice at 8 weeks of age reared separately was associated with increased proportion of CD4+ LAP+ Foxp3- cells and less severe TNBS-colitis. In co-housed mice the acquisition of Rickenellaceae by Nod2+/+ mice was associated with increased CD4+ LAP+ Foxp3- proportion and less severe colitis. Severe colitis was associated with enrichment of gram-negative pathobionts (Escherichia and Enterococcus), while less severe colitis with protective bacteria (Barnesiella, Odoribacter and Clostridium IV). Environmental factors acting on genetic background with different outcomes according to their impact on microbiota, predispose in different ways to inflammation. These results open a new scenario for therapeutic attempt to re-establish eubiosis in Inflammatory Bowel Disease patients with NOD2 polymorphisms

Adaptive Dispersion Compensation for Remote Fiber Delivery of NIR Femtosecond Pulses

We report on remote delivery of 25 pJ broadband near-infrared femtosecond light pulses from a Ti:sapphire laser through 150 meters of single-mode optical fiber. Pulse distortion due to dispersion is overcome with pre-compensation using adaptive pulse shaping techniques, while nonlinearities are mitigated using an SF10 rod for the final stage of pulse compression. Near transform limited pulse duration of 130 fs is measured after the final compression.Comment: 3 pages, 4 figure

Avaliação da seletividade inicial de herbicidas para o tomateiro por meio da análise de fluorescência da clorofila.

Objetivou-se com este trabalho analisar a fluorescência da clorofila por meio da avaliação da taxa de transporte de eletrons em tomateiro como forma de avaliar a seletividade inicial de hervicidas aplicados em pré-transplate

Misure di resistività mediante OhmMapper finalizzate allo studio del sito di Torraccia di Chiusi

The paper describes the findings of a geo-electric study conducted at the archaeological site of Torraccia di Chiusi (Siena Province) with the use of soil resistivity modelling. In collaboration with the Département d’Archéologie et Histoire de l’Art (Université catholique de Louvain, Belgium) and the Centro di GeoTecnologie (Università degli Studi di Siena), a new, non-invasive instrument, named the OhmMapper, was used for the first time in an archaeological context to provide geophysical sections of the terrain. The aim of the study was to evaluate the nature and distribution of the buried anthropogenic structures and to properly locate them on the map without the necessity of excavating. The site, located in the valley of the Foci river, has been described as a long lasting settlement (III to VII centuries A.D., although ceramics dating to the II century B.C. have been uncovered). Belgian and Italian archaeologists, who have been digging at the site for the last two years, have also discovered that it had undergone multiple additions and modifications throughout the years (initially a villa, and later perhaps a mansio). The OhmMapper is a capacitively coupled resistivity meter that measures the electrical properties of rocks and soil without ground stakes used in traditional resistivity surveys. A simple coaxial-cable array with transmitter and receiver sections is pulled along the ground either by a single person or attached to an all-terrain vehicle. The dipole-dipole configuration enables the instrument to immediately pick up the horizontal variations in resistivity, thereby enabling the user to identify structures such as walls or cavities. With the OhmMapper, nine sections were made around the perimeter of the area already excavated to collect data on the presence, depth, and dimensions of anthropogenic structures. The results from the sections were refined and placed on a map using an ArcGIS platform. The interpretation of the nine sections resulted in a new hypothesis on the buried anthropogenic structures and a new model of the natural landscape that existed at the site prior to its construction

The sea urchin embryo: A model to study Alzheimer’s beta amyloid induced toxicity

Alzheimer’s disease (AD) is the most common form of dementia. The cause of AD is closely related to the accumulation of amyloid beta peptide in the neuritic plaques. The use of animal model systems represents a good strategy to elucidate the molecular mechanism behind the development of this pathology. Here we use the Paracentrotus lividus embryo to identify molecules and pathways that can be involved in the degenerative process. As a first step, we identified the presence of an antigen related to the human APP, called PlAPP. This antigen, after gastrula stage, is processed producing a polypeptide of about 10 kDa. By immunohistochemistry we localized the PlAPP antigen in some serotonin expressing cells. Similarly, after 48 or 96 h incubation, a recombinant b-amyloid peptide, rAb42, accumulates around the intestinal tube and oesophagus. In addition, incubation of sea urchin embryos with two different solutions rich in oligomers and fibrillar aggregates of rAb42 induce activation of apoptosis as detected by TUNEL assay. Moreover, we demonstrate that aggregates induce apoptosis by extrinsic pathway activation, whereas oligomers induce apoptosis both by extrinsic and intrinsic pathway activation. Utilizing an apoptotic inhibitor, caspases activation was offset and morphological damage rescued. Taken together all these observations suggest that the sea urchin may be a simple and suitable model to characterize the mechanism underlining the cytotoxicity of Ab42

Characterizing horizontal variability and energy spectra in the Arctic Ocean halocline

Energy transfer from the atmosphere into the upper Arctic Ocean is expected to become more efficient as summer sea-ice coverage decreases and multiyear ice thins due to recent atmospheric warming. However, relatively little is known about how energy is transferred within the ocean by turbulent processes from large to small scales in the presence of ice and how these pathways might change in future. This study characterises horizontal variability in several regions of the Eurasian Arctic Ocean under differing sea-ice conditions. Historic along track CTD data collected by a Royal Navy submarine during summer 1996 allows a unique examination of horizontal variability and associated wavenumber spectra within the Arctic Ocean halocline. Spectral analysis indicates that potential energy variance under perennial sea-ice in the Amundsen Basin is O(100) less than within the Marginal Ice Zone (MIZ) of Fram Strait. Spectra from all regions show a transition in scaling at wavelengths of approximately 5 to 7 km. At scales greater than the transition wavelength to 50 km, energy spectra are consistent with a k-3 scaling (where k is wavenumber) and interior quasi-geostrophic dynamics. The scaling of spectra at these scales is extremely similar between regions suggesting similar dynamics and energy exchange pathways. The k-3 scaling is steeper than typically found in regions of mid latitude open ocean. At scales below the transition wavelength to 300 m, spectra are close to a k-5/3 scaling or flatter, indicating a change in dynamics, which is potentially due to internal waves dominating variability at small scales

Driving magnetic order in a manganite by ultrafast lattice excitation

Optical control of magnetism, of interest for high-speed data processing and storage, has only been demonstrated with near-infrared excitation to date. However, in absorbing materials, such high photon energies can lead to significant dissipation, making switch back times long and miniaturization challenging. In manganites, magnetism is directly coupled to the lattice, as evidenced by the response to external and chemical pressure, or to ferroelectric polarization. Here, femtosecond mid-infrared pulses are used to excite the lattice in La0.5Sr1.5MnO4 and the dynamics of electronic order are measured by femtosecond resonant soft x-ray scattering with an x-ray free electron laser. We observe that magnetic and orbital orders are reduced by excitation of the lattice. This process, which occurs within few picoseconds, is interpreted as relaxation of the complex charge-orbital-spin structure following a displacive exchange quench - a prompt shift in the equilibrium value of the magnetic and orbital order parameters after the lattice has been distorted. A microscopic picture of the underlying unidirectional lattice displacement is proposed, based on nonlinear rectification of the directly-excited vibrational field, as analyzed in the specific lattice symmetry of La0.5Sr1.5MnO4. Control of magnetism through ultrafast lattice excitation has important analogies to the multiferroic effect and may serve as a new paradigm for high-speed optomagnetism.Comment: 10 pages manuscript, 4 figure

Ectopic hbox12 Expression Evoked by Histone Deacetylase Inhibition Disrupts Axial Specification of the Sea Urchin Embryo

Dorsal/ventral patterning of the sea urchin embryo depends upon the establishment of a Nodal-expressing ventral organizer. Recently, we showed that spatial positioning of this organizer relies on the dorsal-specific transcription of the Hbox12 repressor. Building on these findings, we determined the influence of the epigenetic milieu on the expression of hbox12 and nodal genes. We find that Trichostatin-A, a potent and selective histone-deacetylases inhibitor, induces histone hyperacetylation in hbox12 chromatin, evoking broad ectopic expression of the gene. Transcription of nodal concomitantly drops, prejudicing dorsal/ventral polarity of the resulting larvae. Remarkably, impairing hbox12 function, either in a spatially-restricted sector or in the whole embryo, specifically rescues nodal transcription in Trichostatin-A-treated larvae. Beyond strengthen the notion that nodal expression is not allowed in the presence of functional Hbox12 in the same cells, these results highlight a critical role of histone deacetylases in regulating the spatial expression of hbox12

Dessins, their delta-matroids and partial duals

Given a map $\mathcal M$ on a connected and closed orientable surface, the delta-matroid of $\mathcal M$ is a combinatorial object associated to $\mathcal M$ which captures some topological information of the embedding. We explore how delta-matroids associated to dessins d'enfants behave under the action of the absolute Galois group. Twists of delta-matroids are considered as well; they correspond to the recently introduced operation of partial duality of maps. Furthermore, we prove that every map has a partial dual defined over its field of moduli. A relationship between dessins, partial duals and tropical curves arising from the cartography groups of dessins is observed as well.Comment: 34 pages, 20 figures. Accepted for publication in the SIGMAP14 Conference Proceeding

Arranjo de plantas no desempenho do híbrido de tomate para processamento industrial BRS Sena.

Objetivou-se com este trabalho avaliar diferentes arranjos de plantas para o híbrido de tomate para processamento industrial BRS Sena, considerando duas épocas de transplante: mês de fevereiro (ano de 2012) e mês de abril (ano de 2013).Resumo 1