DNA aneuploidy as a topographic malignant transformation pattern in a pleomorphic adenoma of long-term evolution: a case report

<p>Abstract</p> <p>Introduction</p> <p>We present a case of long-term evolution of a submandibular pleomorphic adenoma. There is little information about topographic malignant transformation patterns of pleomorphic adenomas.</p> <p>Case presentation</p> <p>We extensively analyze a giant submandibular mixed tumor of 25-year evolution in a 57-year-old Caucasian woman. Deoxyribonucleic acid ploidy was evaluated in different superficial and deep areas using flow cytometry analysis and correlated with pathological and immunohistochemical characteristics. Superficial areas exhibited a typical histological pleomorphic adenoma pattern and were deoxyribonucleic acid diploid. Deep samples showed deoxyribonucleic acid aneuploidy, atypical histological benign features and expression of markers involved at an early-stage of malignant transformation, such as tumor protein 53 and antigen Ki67.</p> <p>Conclusion</p> <p>These findings revealed that deep tumor compartments may be involved in the initial stages of malignant transformation. Deoxyribonucleic acid ploidy analysis may provide an additional diagnosis tool and indicate 'uncertain' areas that require careful study to avoid diagnostic errors. Larger studies are needed to confirm our results and to evaluate the usefulness of the technique.</p

Sorodiscordância na atenção às pessoas com HIV/AIDS: implicações para o enfermeiro Serodiscordance in care for people with HIV/AIDS: implications for nurses

Objetivo: analisar a produção científica sobre a prática sexual em casais sorodiscordantes e destacar as implicações para a prática do enfermeiro. Método: trata-se de uma revisão integrativa realizada nas bases de dados da Biblioteca Virtual em Saúde, Lilacs, SciELO, PubMED, CINAHL, sendo selecionados 12 artigos que atenderam aos critérios de inclusão, publicados de 2009 a 2014. Resultados: a maioria dos artigos foram publicados por enfermeiros em 2011 e 2013 nas revistas Caderno de Saúde Pública, Temas em Psicologia e Revista da Escola de Enfermagem USP; sendo prevalente o descritor, casamento. As publicações foram agrupadas em duas categorias temáticas: Práticas sexuais após o diagnóstico do HIV; e Sorodiscordância na vida afetivo-sexual de portadores do HIV/AIDS: implicações para o enfermeiro. Conclusões: ações de enfermagem pautadas na orientação sexual contribuam para melhorar a qualidade de vida dos sorodiscordantes

miR-625-3p upregulation is a late event after T cell activation.

<p>(A-D) CD8+ T cells isolated from PBMCs of 4 healthy donors were stimulated with PHA + 120 IU/ml IL-2 and (A) CD69, CD25 and CD79 surface expressions, (B) IFN-γ in the supernatant, (C) BrdU uptake and (D) intracellular miR-625-3p expression were measured after 0h, 6h, 1, 3, 5 and 7 days. Y-axis: (A) Mean fluorescence intensity (MFI), (B) Relative uptake of BrdU calculated as [absorbance of sample—absorbance of unstimulated control]/absorbance of unstimulated control, (C) IFN-γ levels in pg/ml, (D) Relative fold change in miR-625-3p expression calculated by 2^-ΔΔct method using RNU48 and U6 snRNA as reference genes. (A-C) Statistical analysis was calculated by paired t-test; *indicates p<0.05, **indicates p<0.01. (D) Fold change above dotted line indicates significant fold change value >2. Data are representative of three independent experiments. All measurements were performed in duplicates.</p

miR-625-3p expression is regulated during CD8+ T cell reconstitution in vivo.

<p>(A) Viable CD8+ T cells were quantified (grey triangles) and isolated by FACS sorting from peripheral blood samples collected on days 25 (n = 22), 45 (n = 53), 90 (n = 45) and 150 (n = 17) after allogeneic SCT. Subsequently, the relative miR-625-3p expression (black circles) was determined. Left Y-axis: Mean relative miR-625-3p expression calculated by miR-625-3p [RQ]/mean RNU48/U6 snRNA [RQ]. RQ: relative quantity. Right Y-axis: Mean CD8+ T cell count /μl at the time of blood collection (±5 days). Error bars indicate standard error of mean. All measurements were performed in duplicates. Statistical comparisons was calculated in comparison with the miR-625-3p expression in CD8+ T cells of healthy donors by Mann-Whitney U test; *indicates p<0.05. (B) Correlation analysis between relative miR-625-3p expression in patient derived CD8+ T cells (black circles) and the exact time of collection after allogeneic SCT is shown. The relationship between collection time and miR-625-3p expression in CD8+ T cells was analyzed by Spearman correlation coefficient (R²) analysis.</p

Patient characteristics.

<p>Patient characteristics.</p