A Study on Evaluation of Plasma D-Dimer as an Initial Diagnostic Biomarker in Cerebral Venous Thrombosis

BACKGROUND : Cerebral venous thrombosis (CVT) is a life threatening condition with varied clinical presentations. It is mainly a diagnosis by means of radiological tool. CT Brain is the initial investigation of choice. It may be normal in 30–40% of the population. MRI with MRV is the gold standard for the diagnosis of CVT. D Dimer is a product of fibrinolysis which is usually used as an exclusion marker of venous thrombosis. Our study aims to analyse the usefulness of D Dimer as an initial diagnostic marker in CVT. OBJECTIVES: 1. To estimate the level of plasma D-DIMER and its significance in diagnosing Cerebral Venous Thrombosis. 2. To correlate the relationship between plasma D-DIMER and MRI BRAIN in diagnosing Cerebral Venous Thrombosis. 3. To evaluate the diagnostic value of negative D-DIMER in ruling out Cerebral Venous Thrombosis. METHODOLOGY : The patients diagnosed to have CVT by MRI in Coimbatore Medical College above 18 yrs of age are included in the study. A total of 50 patients are included in the study. After confirming the diagnosis, blood samples were drawn, plasma separated and sent to laboratory. D Dimer tests were measured by ELISA test. A value of more than 500 ng/ml were considered positive. All the tests were entered in a data collection sheet in an Excel format and analysed using SPSS software. RESULTS : Out of the 50 patients, 28 were females, and 28 were above 35 yrs. The common presenting symptom is headache (96%), followed by seizures, altered sensorium, with papilledema (60%). The most common sinus involved is superior sagittal sinus (70%) followed by sigmoid and transverse sinus. CT Brain was normal in 50% of the patients. Inherited thrombophilias contribute to 23%. D Dimer was positive in 78% of the study population. D Dimer and MRV has a highly significant correlation (0.034). D Dimer and onset of symptoms have a very high significant value of 0.000. CONCLUSION : D Dimer can be used as an initial diagnostic bio marker in CVT as there is a significant association with MRI. If patients present very early D Dimer is a highly reliable investigation. Further studies needed for the supportive evidence

USING NEURAL MACHINE TRANSLATION TO TRANSLATE BETWEEN DIFFERENT SIGN LANGUAGE FORMS DURING A VIDEO CONFERENCE

Techniques are presented herein that support the efficient conversion of signs from one form of sign language to another while considering the cultural context (e.g., dialect, etc.) of a sign language. Aspects of the presented techniques support the conversion between different sign language forms through a neural machine translation (NMT)-based architecture. Further aspects of the techniques may encompass a contextual frame sampler (which may employ a sign language image database to filter out noise and which may sample frames from an input sign language video), an image normalizer (that may accept as input a sampled image frame and produce as output a skeletal structure of that frame), a translation layer (which may contain a NMT-based model and which may comprise feature extraction, feature conversion, and feature generation capabilities), and a video generator (which may stitch together the generated translated sign language output frames into a video). Under still further aspects of the techniques, such a conversion capability may be available during a video conference

Chemical profiling of fern Cheilosoria mysurensis (Wall. ex Hook.) Ching & Shing and its biological activity

Cheilosoria mysurensis (Wall. ex Hook.) Ching & Shing, a medicinal fern traditionally used to treat burns, throat pain and bone fracture. There is no any scientific report regarding anticancer studies of this species. The aim of the study was to find out the chemical components through GC/MS analysis with their antioxidant and cytotoxic activity. GC-MS analysis shows primary ingredients viz, Cis-9, 10-epoxyoctadecan-1-ol and Oxiraneundecanoic acid, 3-pentyl-, methyl ester, trans were present. High free-radical scavenging activity has been discovered in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and showed the IC50 value of 52.13±0.33. Furthermore, C. mysurensis also showed good cytotoxic effects against DLA and EAC cell lines with values of 72.9% and 79.5% at 200 µg/ml dose respectively. Overall findings suggested that the identified chemical compounds proved to be rich in antioxidant property. Further, this fern can be a good resource for pharma industry to produce novel anti-cancer drugs

Cystatin C and lactoferrin concentrations in biological fluids as possible prognostic factors in eye tumor development

Objectives. To investigate the possible role of cystatin C in eye biological fluids locally and in serum and lactoferrin revealing anti-tumor activity in eye tumor development. Background. The increased number of eye tumors was registered recently not only in the countries with high insolation, but also in the northern countries including Russia (11 cases per million of population). Search for new biological markers is important for diagnosis and prognosis in eye tumors. Cystatin C, an endogenous inhibitor of cysteine proteases, plays an important protective role in several tumors. Lactoferrin was shown to express anti-tumor and antiviral activities. It was hypothesized that cystatin C and lactoferrin could serve as possible biomarkers in the diagnosis of malignant and benign eye tumors. Study design. A total of 54 patients with choroidal melanoma and benign eye tumors were examined (part of them undergoing surgical treatment). Serum, tear fluid and intraocular fluid samples obtained from the anterior chamber of eyes in patients with choroidal melanoma were studied. Methods. Cystatin C concentration in serum and eye biological fluids was measured by commercial ELISA kits for human (BioVendor, Czechia); lactoferrin concentration – by Lactoferrin-strip D 4106 ELISA test systems (Vector-BEST, Novosibirsk Region, Russia). Results. Cystatin C concentration in serum of healthy persons was significantly higher as compared to tear and intraocular fluids. In patients with choroidal melanoma, increased cystatin C concentration was similar in tear fluid of both the eyes. Lactoferrin level in tear fluid of healthy persons was significantly higher than its serum level. Significantly increased lactoferrin concentration in tear fluid was noted in patients with benign and malignant eye tumors. Conclusion. Increased level of cystatin C in tear fluid seems to be a possible diagnostic factor in the eye tumors studied. However, it does not allow us to differentiate between malignant and benign eye tumors. Similar changes were noted for lactoferrin in tear fluid

Noise sensitivity of 89Zr-Immuno-PET radiomics based on count-reduced clinical images

PURPOSE: Low photon count in (89)Zr-Immuno-PET results in images with a low signal-to-noise ratio (SNR). Since PET radiomics are sensitive to noise, this study focuses on the impact of noise on radiomic features from (89)Zr-Immuno-PET clinical images. We hypothesise that (89)Zr-Immuno-PET derived radiomic features have: (1) noise-induced variability affecting their precision and (2) noise-induced bias affecting their accuracy. This study aims to identify those features that are not or only minimally affected by noise in terms of precision and accuracy. METHODS: Count-split (89)Zr-Immuno-PET patient scans from previous studies with three different (89)Zr-labelled monoclonal antibodies were used to extract radiomic features at 50% (S50p) and 25% (S25p) of their original counts. Tumour lesions were manually delineated on the original full-count (89)Zr-Immuno-PET scans. Noise-induced variability and bias were assessed using intraclass correlation coefficient (ICC) and similarity distance metric (SDM), respectively. Based on the ICC and SDM values, the radiomic features were categorised as having poor [0, 0.5), moderate [0.5, 0.75), good [0.75, 0.9), or excellent [0.9, 1] precision and accuracy. The number of features classified into these categories was compared between the S50p and S25p images using Fisher’s exact test. All p values < 0.01 were considered statistically significant. RESULTS: For S50p, a total of 92% and 90% features were classified as having good or excellent ICC and SDM respectively, while for S25p, these decreased to 81% and 31%. In total, 148 features (31%) showed robustness to noise with good or moderate ICC and SDM in both S50p and S25p. The number of features classified into the four ICC and SDM categories between S50p and S25p was significantly different statistically. CONCLUSION: Several radiomic features derived from low SNR (89)Zr-Immuno-PET images exhibit noise-induced variability and/or bias. However, 196 features (43%) that show minimal noise-induced variability and bias in S50p images have been identified. These features are less affected by noise and are, therefore, suitable candidates to be further studied as prognostic and predictive quantitative biomarkers in (89)Zr-Immuno-PET studies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40658-022-00444-4

Performance of little millet (Panicum flexuosum) based cropping system for rainfed agro ecosystems: A path to sustainable crop diversification

An experiment was conducted during 2019, 2020 and 2021 at Centre of Excellence in Millets, (Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu), Athiyandal, Tiruvanamalai, Tamil Nadu. to study the performance of little millet (Panicum flexuosum Retz.) based cropping system for rainfed agro ecosystems for north-eastern part of Tamil Nadu. The experiment was laid out in randomized block design (RBD) comprised of 9 treatments, viz. T1, Little millet + Redgram [Cajanus cajan (L.) Millsp.] (4:1)-Moth bean [Vigna aconitifolia (Jacq.) Marechal]; T2, Little millet + Redgram (4:1)-Blackgram [Vigna mungo (L.) Hepper]; T3, Little millet + Redgram (4:1)-Horsegram [Macrotyloma uniflorum (Lam.) Verdc.]; T4, Little millet + Redgram (6:2)-Moth bean; T5, Little millet + Redgram (6:2)-Blackgram; T6, Little millet + Redgram (6:2)-Horsegram; T7, Little millet + Redgram (8:2)- Mothbean; T8, Little millet + Redgram (8:2)-Blackgram; T9, Little millet + Redgram (8:2)- Horsegram, with three replications. The good quality seeds of little millet (CO 4), redgram (Paiyur 2), horsegram (Paiyur 2), blackgram (VBN 3) and mothbean [TMV (Mb) 1] were used. The result shows that the higher yield for little millet and intercropped redgram was recorded in T9 (985 and 362 kg/ha) followed by T7 (853 and 356 kg/ha) and T8 (834 and 337 kg/ ha). The 8:2 row ratio exhibited the highest little millet equivalent yield when compared to 6:2 and 4:1 row ratio. The highest economic return and benefit cost (B:C) ratio was observed in T9 with gross return of ₹60,962/ha, net returns of ₹35,362/ha and B:C ratio (2.38). This was followed by T7 which had gross returns of ₹51,314/ha, net returns of ₹25,989/ha and B:C ratio (2.10)

Antiulcer, wound healing and hepatoprotective activities of the seaweeds Gracilaria crassa, Turbinaria ornata and Laurencia papillosa from the southeast coast of India

Seaweeds have bioactive compounds of interest in the pharmaceutical industry. In India, seaweeds are used exclusively for phycocolloids production and have not yet received consideration as a dietary supplement. So, it has become imperative to explore the biomedical potential of seaweeds and promote their utilization as a functional food. The seaweeds Turbinaria ornata, Gracillaria crassa and Laurencia papillosa, collected from the Tuticorin coast of the Southeast coast of India and selected based on preliminary screening, were extracted with acetone and evaluated for antiulcer, wound healing and hepatoprotective activities. L. papillosa showed the highest level of gastric protection activity (81%) at 200 mg/kg, comparable to the standard drug ranitidine (90%). G. crassa followed with 76%. G. crassa and L. papillosa, showed marked wound-healing activity. G. crassa at 200 mg/kg, showed a marked effect on the serum marker enzymes indicating prominent hepatoprotective activity. The noteworthy wound-healing and hepato-protective properties of G. crassa besides anti-ulcer activity next to L. papillosa were indicative of its potential for further consideration