Evaluation report of demonstrator

No abstract availabl

Abundância de fungos micorrízicos arbusculares e biomassa total fúngica e bacteriana de solos cultivados com cana de açúcar.

A cana de açúcar movimenta no Brasil cerca de R$40 bilhões por ano com a produção e comercialização de açúcar e etanol. Os micro-organismos do solo têm são fundamentais no ciclo dos nutrientes, destacando os Fungos Micorrízicos Arbusculares (FMAs) no fornecimento de fósforo para as plantas. Os objetivos desse trabalho foram determinar os parâmetros quantitativos da microbiota do solo através da avaliação da abundância de esporos dos FMAs e biomassa total de fungos e bactérias utilizando a técnica de microscopia de Epifluorescência, em mata ciliar e em canaviais com diferentes manejos do solo. Foram selecionadas seis áreas no Estado de São Paulo e as amostras de solo foram coletadas nos períodos de outubro/novembro de 2010. A abundância de esporos dos FMAs junto ao manejo de cana de açúcar não apresentaram diferenças significativas. Houve aumento significativo do pH do solo nos solos manejados em relação com a mata ciliar. O baixo pH do solo não interferiu na comunidade dos FMAs e também não afetou na biomassa de fungos e bactérias. Foram observadas diferenças significativas no aumento para biomassa de bactérias e fungo+bactérias do solo no manejo com cana queimada, com aplicação de vinhaça e colheita mecanizada em relação à cana crua, sem vinhaça e colheita manual. Este é o primeiro estudo que se tem conhecimento realizado no Brasil utilizando a técnica de microscopia de epifluorescência para estimar a biomassa total de carbono de fungos e bactérias e os resultados apresentados aqui estão de coerentes com outros estudos da literatura. Desse modo a técnica é promissora e poderá ser utilizada em futuros estudos no Brasil.Fertbio

Technical report of data mining

No abstract availabl

Three dimensional fluid-kinetic model of a magnetically guided plasma jet

A fluid-kinetic model of the collisionless plasma row in a convergent-divergent magnetic nozzle is presented. The model combines the leading-order Vlasov equation and the fluid continuity and perpendicular momentum equation for magnetized electrons, and the fluid equations for cold ions, which must be solved iteratively to determine the self-consistent plasma response in a three-dimensional magnetic field. The kinetic electron solution identifies three electron populations and provides the plasma density and pressure tensor. The far downstream asymptotic behavior shows the anisotropic cooling of the electron populations. The fluid equations determine the electric potential and the fluid velocities. In the small ion-sound gyroradius case the solution is constructed one magnetic line at a time. In the large ion-sound gyroradius case, ion detachment from magnetic lines makes the problem fully three-dimensional.This work was supported by National R&D Plan (Grant ESP2016-75887) from the Gobierno de España. Jesús Ramos thanks the financial sponsorship of the Chair of Excellence award granted by UC3M and Banco de Santander

Thrombospondin-1 is downregulated by anoxia and suppresses tumorigenicity of human glioblastoma cells.

Angiogenesis, the sprouting of new capillaries from preexisting blood vessels, results from a disruption of the balance between stimulatory and inhibitory factors. Here, we show that anoxia reduces expression of thrombospondin-1 (TSP-1), a natural inhibitor of angiogenesis, in glioblastoma cells. This suggests that reduced oxygen tension can promote angiogenesis not only by stimulating the production of inducers, such as vascular endothelial growth factor, but also by reducing the production of inhibitors. This downregulation may significantly contribute to glioblastoma development, since we show that an increase in TSP-1 expression is sufficient to strongly suppress glioblastoma cell tumorigenicity in vivo

Synthesis and preliminary in vitro evaluation of DOTA-Tenatumomab conjugates for theranostic applications in tenascin expressing tumors

Tenatumomab is an anti-tenascin murine monoclonal antibody previously used in clinical trials for delivering radionuclides to tumors by both pre-targeting (biotinylated Tenatumomab within PAGRIT) and direct 131Iodine labeling approaches. Here we present the synthesis and in vitro characterization of three Tenatumomab con-jugates to bifunctional chelating agents (NHS-DOTA, NCS-DOTA and NCS-DTPA). Results indicate ST8198AA1(Tenatumomab-DOTAMA, derived by conjugation of NHS-DOTA), as the most promising candidate in terms ofconjugation rate andyield, stability,antigenimmunoreactivity andaffinity. Labeling efficiency of thedifferentchelators was investigated with a panel of cold metals indicating DOTAMA as the best chelator. Labeling ofTenatumomab-DOTAMA was then optimized with several metals and stability performed confirms suitability of this conjugate for further development. ST8198AA1 represents an improvement of the previous antibody forms because the labeling with radionuclides like177Lu or64Cu would allow theranostic applications in patientsbearing tenascin expressing tumor