Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions

Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.Fil: Gilardoni, Mónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Oviedo, Mabel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Dellavedova, Tristán. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Sarría, Juan P.. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Racca, Laura. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Dominguez, Mariana. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Pellizas, Claudia Gabriela. Universidad Nacional de Córdoba; ArgentinaFil: Donadio, Ana Carolina. Universidad Nacional de Córdoba; Argentin

Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells

The four serotypes of dengue virus (DENV1-4) are the causal agents of the emerging disease Dengue Fever and its severe forms. DENV is inoculated into human blood through a mosquito bite. Thus, plasma is an important media for DENV dissemination in infected persons and several important interactions should take place for the virus with human plasma proteins that strongly influence or may determine the course of the infection. This dataset contains 239 proteins identified in the elution fractions of human plasma subjected to DE-52 anion exchange chromatography. Data on DENV2 infection of Huh 7.5 cells in presence of the human plasma fraction is also presented. Keywords: Human plasma, Anion exchange chromatography, Dengue viru