Trace Element Patterns in Shells of Mussels (Bivalvia) Allow to Distinguish between Fresh- and Brackish-Water Coastal Environments of the Subarctic and Boreal Zone

The accumulation of trace metals in the shells of bivalves allows quantitative assessments of environmental pollution and helps to reconstruct paleo aquatic environments. However, the understanding on how marine and freshwater mollusks control the level of trace elements in their shells remains very limited. Here, we compared the trace element composition of marine and freshwater bivalves from boreal and subarctic habitats, using examples of widely distributed species of marine (Mytilus edulis, M. trossulus) and freshwater (Anodonta anatina, Unio sp., Beringiana beringiana) mussels. Sizable differences in several trace element concentrations were detected between different species, depending on their environmental niches. A multiparametric statistical treatment of the shell’s elemental composition allowed to distinguish the impact of external factors (water and sediment chemical composition) from active metabolic (biological) control. In particular, the obtained results demonstrated that Ba:Ca and Pb:Ca ratios in mussels’ shells are closely related to the primary productivity of aquatic ecosystems. The Mn:Ca ratio allowed to constrain the environmental conditions of mussels’ species depending on the trophic state of inhabited waterbody. Overall, the marine mussels exhibited stronger biological control of trace element accumulation, whereas trace element pattern in shells of freshwater mussels was chiefly controlled by environmental factors. The obtained results might help to use the trace element composition of bivalves in distinguishing marine and freshwater habitats of mollusks in paleo environments

Iron, Phosphorus and Trace Elements in Mussels’ Shells, Water, and Bottom Sediments from the Severnaya Dvina and the Onega River Basins (Northwestern Russia)

Trace elements in freshwater bivalve shells are widely used for reconstructing long-term changes in the riverine environments. However, Northern Eurasian regions, notably the European Russian North, susceptible to strong environmental impact via both local pollution and climate warming, are poorly studied. This work reports new data on trace elements accumulation by widespread species of freshwater mussels Unio spp. and Anodonta anatina in the Severnaya Dvina and the Onega River Basin, the two largest subarctic river basins in the Northeastern Europe. We revealed that iron and phosphorous accumulation in Unio spp. and Anodonta anatina shells have a strong relationship with a distance from the mouth of the studied river (the Severnaya Dvina). Based on multiparametric statistics comprising chemical composition of shells, water, and sediments, we demonstrated that the accumulation of elements in the shell depends on the environment of the biotope. Differences in the elemental composition of shells between different taxa are associated with ecological preferences of certain species to the substrate. The results set new constraints for the use of freshwater mussels’ shells for monitoring riverine environments and performing paleo-reconstructions

Freshwater mollusks from Neogene-Quaternary Dniester and Prut riverine deposits as indicator paleoenvironments: chemical composition of shells and its palaeoecological interpretation

The respective environments in two ancient rivers were studied using geochemical methods with paleogeographic reconstructions of fossil material represented by shells of freshwater bivalve mollusks. The studied outcrops are located in the basins of the Dniester and Prut rivers. Materials were collected from two Pliocene (Brînza, Giurgiuleşti) outcrops and the ages of the second group of localities (Sucleia, Slobozia Mare, Gura Bîcului) are from Middle to Late Pleistocene. The determination of the taxonomic position was carried out using standard malacological methods. Geochemical data were used for the environmental reconstruction and included stable isotope ratio and trace element compositions in subfossil freshwater bivalve shell (Bivalvia: Unionoida). Key indicators of paleoenvironments show changes in water temperature. Changes in the taxonomic composition of bivalve mollusk assemblages also occurred. In the present study, increasing the water temperature caused a change of stenobiont species of bivalve mollusks to eurybiont species. Eutrophication of watercourses, caused by anthropogenic pollution and climate change in the direction of warming, has led to the replacement of pearl mussels of the family Margaritiferidae by more eurybiont species of the family Unionidae in the benthic communities of European oligotrophic rivers. These processes are similar to those deduced for the Neogene-Quaternary watercourses of the Prut and Dniester basins, but they proceed at a much faster pace. They are processes of eutrophication of watercourses – the main factor leading to the catastrophically rapid modern reduction of the ranges of pearl mussel

Symbiotic cooperation between freshwater rock-boring bivalves and microorganisms promotes silicate bioerosion

International audienceBioerosion is a process with a high socioeconomic impact that contributes to coastal retreat, and likely to increase with climate change. Whereas limestone bioerosion is well explained by a combination of mechanical and chemical pathways, the bioerosion mechanisms of silicates, which are harder and chemically more resistant, remain elusive. Here we investigated the interface between siltstone and freshwater rock-boring bivalves Lignopholas fluminalis (Bivalvia: pholadidae). Remains of a microbial biofilm were observed only in the poorly consolidated part of the rock within the macroborings created by bivalves. Secondary Mn-bearing minerals identified in the biofilm suggest that microbes promoted silicate rock weathering by dissolving Mn-rich chlorites. Moreover, hard mineral debris found in a biofilm attached to the shells likely contributed to the abrasion of the rock substrate. thus, beyond the classical view of chemical and/or mechanical action(s) of macroborers, silicate bioerosion may also be facilitated by an unexpected synergistic association between macro-and microorganisms. Bioerosion is a commonplace strategy developed by living organisms, which consists in boring hard substrates of various origins, including biological materials (e.g., wood, shells, and bones) 1 , mud 2 , rocks 3 and even synthetic materials. Depending on the nature of the substrate and the borer, bioerosion ensures a wide range of metabolic activities and ecosystem services, ranging from nutrition 4 to the creation of microhabitats protected from predators for themselves as well as for secondary dwellers 5. Gaining knowledge into the occurrence, rates and mechanisms of boring is of fundamental importance for a series of reasons. First and historically, mankind has been confronted with macroborers through the damages caused by shipworms on vessels, wooden wharfs and docks 5,6. More broadly, bioerosion has socioeconomic impacts whenever manufactured materials are damaged, including plastic, metals and concrete materials such as levees or coastal defences 3,6. Second, bioerosion contributes to element recycling, shaping landscapes through the weakening of rocky shorelines and participating to coastal retreat 5 , for which the current rates are likely to be modified drastically as a result of climate change 6. Third, the creation of microhabitats by macroborers such as bivalves is correlated with a significant increase of the abundance of species assemblages, thus partly contributing to local faunal biodiversity 7. Finally, fossil records of macro-bioerosion may be used as a biological proxy to estimate the paleo-location of intertidal and shallow subtidal marine environments, marking ancient shorelines 8. The mechanisms of rock bioerosion associated to macroborers and especially bivalves have been a source of lively debate for decades, and can be schematically divided into two main pathways. First, rock boring can ope

Taxonomic Reassessment of Freshwater Mussels from the Western Balkans Reveals an Overlooked but Critical Refugium and Defines Conservation Priorities

Freshwater mussels are an endangered group of animals, especially in the water bodies of Central and Western Europe. Conservation priorities were established for many endangered freshwater mussel species in the last decade. Here, we present new data on a cryptic refugium of freshwater mussels in the Western Balkans. Two species, Anodonta exulcerata Porro, 1838 and Unio elongatulus Pfeiffer, 1825, were discovered in Montenegro for the first time. The phylogenetic position and taxonomic status of freshwater mussel species from Montenegro were studied by means of an integrative approach, combining morphological data and COI gene sequences. Unio carneus Küster, 1854 stat. rev. represents a species with a restricted range, which is endemic to the Western Balkans. A viable population of Microcondylaea bonellii (Férussac, 1827) was discovered in the Zeta River. This species was not mentioned in Montenegro since the 1900s. A significant level of genetic diversity was revealed for the studied species. In the Skadar Lake basin, freshwater mussels from diverse ecological groups were discovered. Rheophilic species of freshwater mussels may represent ancient lineages, which need special conservation planning. Conservation priorities should be aimed at the protection of freshwater mussel habitats on the Balkan Peninsula

Taxonomic Reassessment of Freshwater Mussels from the Western Balkans Reveals an Overlooked but Critical Refugium and Defines Conservation Priorities

Freshwater mussels are an endangered group of animals, especially in the water bodies of Central and Western Europe. Conservation priorities were established for many endangered freshwater mussel species in the last decade. Here, we present new data on a cryptic refugium of freshwater mussels in the Western Balkans. Two species, Anodonta exulcerata Porro, 1838 and Unio elongatulus Pfeiffer, 1825, were discovered in Montenegro for the first time. The phylogenetic position and taxonomic status of freshwater mussel species from Montenegro were studied by means of an integrative approach, combining morphological data and COI gene sequences. Unio carneus Küster, 1854 stat. rev. represents a species with a restricted range, which is endemic to the Western Balkans. A viable population of Microcondylaea bonellii (Férussac, 1827) was discovered in the Zeta River. This species was not mentioned in Montenegro since the 1900s. A significant level of genetic diversity was revealed for the studied species. In the Skadar Lake basin, freshwater mussels from diverse ecological groups were discovered. Rheophilic species of freshwater mussels may represent ancient lineages, which need special conservation planning. Conservation priorities should be aimed at the protection of freshwater mussel habitats on the Balkan Peninsula

New paleogeography data of the eastern coast of Green fjord (West Spitsbergen Island) based on research of the holocene deposits on Finneset cape with use of isotope-geochemical methods

Актуальность исследования заключается в определении возраста моллюсков в заливе Грен-фьорд на архипелаге Шпицберген и физико-химических параметров среды их обитания в условиях угрозы разрушения геологических обнажений с находящимися в них моллюсками и костным материалом птиц и рыб в результате активно развивающихся процессов береговой термоабразии и техногенной нагрузки комбината «Арктикуголь». Цель: реконструкция основных физико-химических параметров среды обитания моллюсков в голоцене на основе определения видовой принадлежности моллюсков и изучения изотопно-геохимического состава раковин и вмещающих их горных пород. Методы: определение видовой принадлежности раковин моллюсков с использованием морфологического метода; разложение образцов раковин путем кислотного вскрытия; определение содержания макро- и микроэлементов на масс-спектрометре с индуктивно связанной плазмой Aurora Elite фирмы Bruker (Германия) и на волнодисперсном рентгенфлуоресцентном спектрометре LabCenter XRF-1800; определение отношений стабильных изотопов на масс-спектрометре Finningan МАТ253; определение возраста раковин моллюсков на жидкостном низкофоновом сцинтилляционном счетчике. Объекты: четвертичные отложения берегового клифа мыса Финнисет (залив Грён-фьорд, арх. Западный Шпицберген), раковины двустворчатых моллюсков и их фрагменты Результаты. Определены обнаруженные комплексы макрофауны, представленные двустворчатыми моллюсками (Hiatella arctica, Mytilus edulis, Tridonta borealis, Mya truncata). Впервые для мыса Финнисет установлен возраст ископаемых раковин морских моллюсков, который составляет от 7970 до 8050 кал. лет. Установлено, что моллюски, найденные в нижних слоях, обитали в более теплой среде чем те, что обнаружены в вышележащих. Реконструированы редокс-условия с помощью соотношения Ni/Co в пределах от 1,5 до 9 единиц и V/Cr менее 2 единиц. Определено содержание U, которое изменяется в диапазоне от 0,2 мг до 4 мг. Анализ отношения Сu/Cr в разрезе показал, что расстояние от области сноса до области осадконакопления изменялось незначительно. Содержания Zn в горной породе свидетельствует о временной регрессии моря и преобладании континентального режима осадконакопления над морским. Определение концентраций Mn в раковинах моллюсков показало, что они обитали в прибрежно-морской зоне с высоким содержанием кислорода.The relevance of the research consists in determining the age of mollusks in the Green fjord bay of West Spitsbergen Island and physical and chemical parameters of their habitant environment in conditions of the threat of destruction of geological outcrops as a result of actively developing processes of coastal thermo-abrasion and man-made load of «Arcticugol» combine. The main aim of the research is to reconstruct physical and chemical parameters of mollusks habitant environment in Holocene based on determination of mollusks species and research of isotope-geochemical composition of shells and their host rocks. Methods. Mollusk species were identified using morphological method and decomposition of shell samples - by acid dissection. Content of microelements and microelements was determined on Aurora Elite inductively coupled plasma mass spectrometer (Bruker, Germany) and on LabCenter XRF-1800 wavelength X-ray fluorescence spectrometer and stable isotope ratios - on Finningan MAT253 mass spectrometer. Age of mollusk shells was identified on liquid low-background scintillation counter. Objects of the research are quaternary sediments of the coastal cliff of Finniset cape (Green fjord bay, West Spitsbergen Island), shells of bivalve mollusks and their fragments. Results. The authors have identified the detected macrofauna complexes represented by bivalve mollusks (Hiatella arctica, Mytilus edulis, Tridonta borealis, Mya truncata) and the age of fossil shells of marine mollusks for Finniset cape, which are from 7970 to 8050 years old. It was established that the mollusks found in lower layers lived in a wormer climate than those found in the overlying layers. The authors reconstructed redox conditions using Ni/Co ratios ranging from 1,5 to 9 units and V/Cr less than 2 units. Content of the U was detected in range from 0,2 mg to 4 mg. An analysis of the Cu/Cr ratio in the outcrop shows that the distance from the area of denudation to the sedimentation area changed insignificantly. Zn concentration in sediments indicates the temporal regression of the sea and predominance of continental sedimentary regime over sea regime. Determination of Mn content in mollusk shells shown, that they lived in coastal-marine zone with high oxygen content

Cryptic taxonomic diversity and high-latitude melanism in the glossiphoniid leech assemblage from the Eurasian Arctic

Abstract The family Glossiphoniidae is a diverse and widespread clade of freshwater leeches, playing a significant role in functioning of aquatic ecosystems. The taxonomy and biogeography of leeches from temperate, subtropical, and tropical regions attracted much attention of zoologists, while their taxonomic richness and distribution in the Arctic are poorly understood. Here, we present an overview of the Eurasian Arctic Glossiphoniidae based on the most comprehensive occurrence and DNA sequence datasets sampled to date. This fauna contains 14 species, belonging to five genera and three subfamilies. One genus and five species are new to science and described here. The world’s northernmost occurrences of glossiphoniids are situated on the Taymyr Peninsula at 72° N, although further records at higher latitudes are expected. Most Arctic leeches are characterized by broad ranges crossing several climatic zones (e.g., Glossiphonia balcanica and G. nebulosa), although the distribution of two new species may be confined to the high-latitude areas. The Taymyr Peninsula with the nearby Putorana Plateau represents the most species-rich area (totally 9 species), while the European Arctic, Iceland, Kolyma Highland, and Chukotka Peninsula house depleted faunas (2–4 species per subregion). Finally, we show that the high-latitude melanism is a common phenomenon in glossiphoniid leeches