14 research outputs found

    The Deployment Of Directed Energy Weapons In Outer Space: An Evaluation

    Get PDF
    This thesis evaluates the logic of deploying directed energy weapons in outer space for use in anti-satellite and in anti-ballistic missile operations. The purpose of this evaluation is to determine if it is in the best interests of the United States to deploy directed energy weapons or to seek a treaty which would ban the development of such weapons

    Comparison of General Fungal and Basidiomycete-specific ITS Primers for Identification of Wood Decay Fungi

    No full text
    The identity of the fungi associated with and responsible for wood decay could lead to the development of more environmentally benign wood preservative systems. In this study we have reconstructed the phylogenetic tree of a select group of basidiomycete fungi using nucleotide sequences of the internal transcribed spacer (ITS) region. We then compared this to trees generated from restriction fragment length polymorphism (RFLP) analysis using two different primers to see if the latter procedures can correctly identify multiple isolates of select wood decay fungi. The phylogenetic tree using maximum likelihood analysis revealed three well-supported genera, Trametes, Phanerochaete, and Gloeophyllum, with bootstrap values of 75 or greater. Trametes and Phanerochaete were sister taxa, and Gloeophyllum was a sister taxon to the Trametes/Phanerochaete clade. Neither set of RFLP data could resolve the three genera into monophyletic groups. The RFLP tree based on general fungal primers also did not resolve species, while the basidiomycete-specific data could resolve species. In the basidiomycete-specific tree, all isolates of both G. striatum and G. trabeum comprised monophyletic groups. Eight of nine T. versicolor isolates, 10 of 11 G. sepiarium isolates, and nine of 10 T. hirsuta isolates comprised monophyletic groups. Phanerochaete could not be consistently resolved into monophyletic groups at either the generic or specific level. Our studies indicate that RFLP analysis using general fungal primers are not likely to be useful in identifying species or reconstructing phylogenetic relationships. RFLP analysis using basidiomycete-specific primers may be useful in identifying some species but not in reconstructing phylogenetic relationships although it is a simpler procedure than sequencing
    corecore