The use of jclic in the english teaching-learning process

This Master Thesis presents the results of a research project carried out at the Primary School (C.E.I.P) Ferrer Guardia in Almeria with 5th grade class A and 5th grade class B students in order to verify if there is an impact of JClic at this school. The present paper proposes a mainly investigative objective; which is to demonstrate that the JClic programme can be an educational resource designed to improve the children’s abilities and skills so as to develop the Teaching-Learning process in the English classroom. Starting from a theoretical basis exploration in order to place and define the research aim, the different necessary stages of this research will be described, explaining and justifying at the same time the methodology and data used. In the end, the obtained results and conclusions will be analysed with the objective to establish possible improvement proposals for the JClic programme as an educational resource

Building a network of TP53 and IGHV testing reference centers across Spain: the Red53 initiative

© The Author(s) 2021.Among the different biomarkers predicting response in chronic lymphocytic leukemia (CLL), the most influential parameters are the mutational status of the IGHV genes and the presence of TP53 gene disruptions. Nevertheless, these important assessments are not readily available in most centers dealing with CLL patients. To provide this molecular testing across the country, the Spanish Cooperative Group on CLL (GELLC) established a network of four analytical reference centers. A total of 2153 samples from 256 centers were analyzed over a period of 30 months. In 9% of the patients, we found pathological mutations in the TP53 gene, whereas 48.96% were classified as IGHV unmutated. Results of the satisfaction survey of the program showed a Net Promoter Score of 85.15. Building a national network for molecular testing in CLL allowed the CLL population a broad access to complex biomarkers analysis that should translate into a more accurate and informed therapeutic decision-making.This work was supported in part by Janssen Pharmaceutical Companies of Johnson & Johnson. MC holds a contract from Ministerio de Ciencia, Innovación y Universidades (RYC-2012-12018)

Diversity of immune responses in children highly exposed to SARS-CoV-2

Background: Children are less susceptible than adults to symptomatic COVID‐19 infection, but very few studies addressed their underlying cause. Moreover, very few studies analyzed why children highly exposed to the virus remain uninfected. Methods: We analyzed the serum levels of ACE2, angiotensin II, anti-spike and anti-N antibodies, cytokine profiles, and virus neutralization in a cohort of children at high risk of viral exposure, cohabiting with infected close relatives during the lockdown in Spain. Results: We analyzed 40 children who were highly exposed to the virus since they lived with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected relatives during the lockdown for several months without taking preventive measures. Of those, 26 reported mild or very mild symptoms. The induced immune response to the virus was analyzed 3 months after the household infection. Surprisingly, only 15 children had IgG anti-S (IgG+) determined by a sensitive method indicative of a past infection. The rest, negative for IgG anti-N or S in various tests, could be further subdivided, according to IgM antibodies, into those having IgM anti-S and IgM anti-N (IgG−IgMhigh) and those having only IgM anti-N (IgG−IgMlow). Interestingly, those two subgroups of children with IgM antibodies have strikingly different patterns of cytokines. The IgMhigh group had significantly higher IFN-α2 and IFN-γ levels as well as IL-10 and GM-CSF than the IgMlow group. In contrast, the IgMlow group had low levels of ACE2 in the serum. Both groups have a weaker but significant capacity to neutralize the virus in the serum than the IgG+ group. Two children were negative in all immunological antibody tests. Conclusions: A significant proportion of children highly exposed to SARS-CoV-2 did not develop a classical adaptive immune response, defined by the production of IgG, despite being in close contact with infected relatives. A large proportion of those children show immunological signs compatible with innate immune responses (as secretion of natural antibodies and cytokines), and others displayed very low levels of the viral receptor ACE2 that may have protected them from the virus spreading in the body despite high and constant viral exposure.This research was funded by grants from “Ministerio de Ciencia e Innovación” (PID-2019104760RB-100); “Comunidad de Madrid” (S2017/BMD-3671. INFLAMUNE-CM; FEDER and COTRAVI-19-CM) to MF; Consejo Superior de Investigaciones Científica, CSIC (CSIC-COV19-108, SGL210235) to MF and UB; CRUE-Supera COVID; the European Development Regional Fund “A way to achieve Europe” (ERDF); Merck, Sharp and Dohme Investigator Studies Program (code MISP# IIS 60257); and Fondo Supera COVID-19 (2020–001) to SSV. The study also received institutional grants from “Fundación Ramón Areces” and “Banco de Santander.”Peer reviewe

ACE2 Serum Levels as Predictor of Infectability and Outcome in COVID-19

Background: COVID‐19 can generate a broad spectrum of severity and symptoms. Many studies analysed the determinants of severity but not among some types of symptoms. More importantly, very few studies analysed patients highly exposed to the virus that nonetheless remain uninfected. Methods: We analysed serum levels of ACE2, Angiotensin II and anti-Spike antibodies in 2 different cohorts at high risk of viral exposure, highly exposed but uninfected subjects, either high risk health care workers or persons cohabiting with infected close relatives and seropositive patients with symptoms. We tested the ability of the sera of these subjects to neutralize lentivirus pseudotyped with the Spike-protein. Results: We found that the serum levels of ACE2 are significantly higher in highly exposed but uninfected subjects. Moreover, sera from this seronegative persons can neutralize SARS-CoV-2 infection in cellular assays more strongly that sera from non-exposed negative controls eventhough they do not have anti-CoV-2 IgG antibodies suggesting that high levels of ACE2 in serum may somewhat protect against an active infection without generating a conventional antibody response. Finally, we show that among patients with symptoms, ACE2 levels were significantly higher in infected patients who developed cutaneous as compared with respiratory symptoms and ACE2 was also higher in those with milder symptoms. Conclusions: These findings suggest that soluble ACE2 could be used as a potential biomarker to predict SARS-CoV-2 infection risk and to discriminate COVID-19 disease subtypes.This research was funded by grants from “Ministerio de Ciencia e Innovación” (SAF2013-42850-R, SAF2016-75988-R and PID-2019104760RB-100; FEDER), “Comunidad de Madrid (S2017/BMD-3671. INFLAMUNE-CM; FEDER) to MF, Consejo Superior de Investigaciones Científica, CSIC (CSIC-COV19-108, SGL210235) to MF and UB, CRUE-Supera COVID, the European Development Regional Fund ‘‘A way to achieve Europe’’ (ERDF), Merck, Sharp & Dohme Investigator Studies Program (code MISP# IIS 60257), and Fondo Supera COVID-19 (2020-001) to SS-V. Institutional grants from “Fundación Ramón Areces” and “Banco de Santander”. This research work was also funded by the European Commission – NextGenerationEU (Regulation EU 2020/2094), through CSIC's Global Health Platform (PTI Salud Global).Peer reviewe

Fomento de la participación de los diferentes agentes implicados en el SGIC: actualización del material informativo, mejora del espacio web y creación de plataforma de gestión de reclamaciones, quejas, sugerencias y felicitaciones

Es la memoria del Proyecto de Innovación número 126 de la convocatoria 2021-2022, titulado "Fomento de la participación de los diferentes agentes implicados en el SGIC: actualización del material informativo, mejora del espacio web y creación de plataforma de gestión de reclamaciones, quejas, sugerencias y felicitaciones"

Point-of-care manufacturing: a single university hospital's initial experience

The integration of 3D printing technology in hospitals is evolving toward production models such as point-of-care manufacturing. This study aims to present the results of the integration of 3D printing technology in a manufacturing university hospital.Analysis and interpretation of the data supported by Project PI18/01625 (Ministerio de Ciencia, Innovación y Universidades, Instituto de Salud Carlos III) and European Regional Development Fund (“Una manera de hacer Europa”)

Kappa free light chains index in multiple sclerosis very long-term prognosis

IntroductionThe role of the kappa-free light chain (kFLC) in the diagnosis of multiple sclerosis (MS) and, to a lesser extent, its role as a medium-term prognostic marker have been extensively studied. This study aimed to explore its potential as a long-term prognostic marker for MS.MethodsWe performed an exploratory retrospective observational study by selecting patients systemically followed up in our MS unit with available cerebrospinal fluid and serum samples at the time of initial evaluation. Two groups were defined: benign MS (bMS), defined as patients with Expanded Disability Status Scale (EDSS) ≤ 3 at 10 years of follow-up, and aggressive MS (aMS), defined as patients with EDSS ≥ 6 at 15 years of follow-up. Clinical variables were collected, and the immunoglobulin G (IgG) index, kFLC index, and oligoclonal bands (OCB) were determined for all patients and compared between the groups.ResultsTwenty bMS and 15 aMS patients were included in this study. Sixty percent (21/35) were female, and the mean age at the time of the first symptom was 31.5 ± 9.45 years, with no statistical differences between groups. Median follow-up time was 19.8 years (Interquartile range, IQR 15.9–24.6). The median EDSS scores at the last follow-up were 1.5 and 7.5 in the bMS and the aMS group, respectively. No statistically significant differences were found in the kFLC index between the two groups (136.6 vs. 140.27, p=0.59). The IgG index was positive in 62.9% of patients (55% bMS vs. 73.3% aMS, p>0.05), and OCB was positive in 88.6% (90% bMS vs. 86.7% aMS, p>0.05). A significant positive correlation was found between IgG and kFLC indices (rs = 0.85, p<0.001).ConclusionGiven the absence of differences between the two groups with opposite disease courses, it is unlikely that the kFLC index is a reliable and powerful marker of long-term prognosis in MS

Transparencia de los procesos de garantía de calidad en la gestión de reclamaciones: estrategias de comunicación y difusión de información a los agentes implicados

Fac. de EducaciónFALSEsubmitte

Investigación joven con perspectiva de género VI

Actas del VI Congreso Internacional de Jóvenes Investigadorxs con perspectiva de género (Getafe, 16 - 18 de junio de 2021) organizado por el Instituto Universitario de Estudios de Género de la Universidad Carlos III de Madrid.El concepto de la vejes en la literatura griega arcaica / María Secades Fonseca se inscribe en el marco del Proyectos de I+D+i del Ministerio de Ciencia e Innovación, titulado Vulnerabilidad intrafamiliar y política en el mundo antiguo y dirigido por Susana Reboreda Morillo y Rosa María Cid López (Ref. PID2020-116349GB-I00).Generando una interpretación del Derecho en clave de igualdad de género / Alicia Cárdenas Cordón y Gloria Serrano Valverde es un trabajo elaborado y presentado en el marco del Proyecto de Investigación RTI2018-10669-B-100 ‘‘GEN-DER: Generando una interpretación del Derecho en clave de igualdad de género’’, enmarcado en el Programa Estatal de I+D+i Orientada a los Retos de la Sociedad- Ministerio de Ciencia e Innovación (2019-2021)