Assessment of Soil Physico-Chemical Properties in Selected Natural Habitats of The Wild Rice (Oryza Longistaminata) and their Effects on the Species Morphological Characters

The aim of this study was to assess variation in some soil physical and chemical properties among four selected natural habitats of the wild rice species (Oryza longistaminata) in Tanzania, and their effects on the species morphological characters. Oryza longistaminata is a perennial wild rice species with agronomically important genes, including genes for tolerance to biotic and abiotic stresses that can be used in rice breeding. In Tanzania O. longistaminata grows sympatrically with the cultivated rice (Oryza sativa) in most rice cultivating areas. The selected natural habitats assessed were located in four districts, namely Bagamoyo, Kibaha, Kilombero and Mbarali. Soil samples were collected at the depth of 0 - 20 cm from the four districts and analysed in the laboratory for soil physico-chemical properties using standard protocols. The species morphological characters were assessed based on the morphological descriptors for wild and cultivated rice species developed by Bioversity International and International Rice Research Institute. One way ANOVA was used to determine the extent of variation in soil physico-chemical properties (parameters) among the four natural habitats of O. longistaminata. Canonical Correspondence Analysis (CCA) was used to determine the effects of assessed soil parameters on the morphological characters of O. longistaminata in the study areas. The study revealed variation in soil physico-chemical properties among the districts. Statistically there were significant differences among the habitats (districts) for most of soil physico-chemical properties investigated. In addition, the assessed soil physico-chemical properties were found to influence variation in morphological characters among O. longistaminata populations from different habitats

IN VITRO ANTIMICROBIAL ASSAY OF PLANTS USED IN TRADITIONAL MEDICINE IN BUKOBA RURAL DISTRICT, TANZANIA.

Plants used in traditional medicine in Bukoba Rural district in Tanzania were evaluated for their in vitro antimicrobial activities. Plant materials from eight plant species (Harungana madagascariensis (Lam) Poir., Jatropha curcas L., Lantana trifolia L., Plectranthus barbatus Andr., Pseudospondias microcarpa Engl., Psorospermum febrifugum Spach, Teclea nobilis Del. and Vernonia adoensis [Warp.] SL) were collected based on ethnomedical information provided by traditional herbal practitioners. Results of the study indicate that extracts from the eight plant species were active against at least one or more of the test organisms (Bacillus subtilis, Staphylococcus aureus [gram positive], Escherichia coli, Pseudomonas aeruginosa [gram negative] and Candida albicans [Yeast]). A profile of secondary metabolites (alkaloids, terpenoids, triterpenes, phenolics, tannins, flavonoids, anthraquinones, flavonols/flavones and /or chalcones, sterols and saponins) was obtained for three plant species (Jatropha curcas L., Plectranthus barbatus Andr., and Pseudospondias microcarpa Engl.). The paper discusses the probable therapeutic basis of these traditional plants based on their secondary metabolite profiles and for the first time draws research attention to Bukoba Rural district as a source for plants with potential pharmaceutical applications

Prevalence of Malaria Parasitemia and Purchase of Artemisinin-Based Combination Therapies (ACTs) among Drug Shop Clients in Two Regions in Tanzania with ACT Subsidies.

Throughout Africa, many people seek care for malaria in private-sector drug shops where diagnostic testing is often unavailable. Recently, subsidized artemisinin-based combination therapies (ACTs), a first-line medication for uncomplicated malaria, were made available in these drug shops in Tanzania. This study assessed the prevalence of malaria among and purchase of ACTs by drug shop clients in the setting of a national ACT subsidy program and sub-national drug shop accreditation program. A cross-sectional survey of drug shop clients was performed in two regions in Tanzania, one with a government drug shop accreditation program and one without, from March-May, 2012. Drug shops were randomly sampled from non-urban districts. Shop attendants were interviewed about their education, training, and accreditation status. Clients were interviewed about their symptoms and medication purchases, then underwent a limited physical examination and laboratory testing for malaria. Malaria prevalence and predictors of ACT purchase were assessed using univariate analysis and multiple logistic regression. Amongst 777 clients from 73 drug shops, the prevalence of laboratory-confirmed malaria was 12% (95% CI: 6-18%). Less than a third of clients with malaria had purchased ACTs, and less than a quarter of clients who purchased ACTs tested positive for malaria. Clients were more likely to have purchased ACTs if the participant was <5 years old (aOR: 6.6; 95% CI: 3.9-11.0) or the shop attendant had >5 years, experience (aOR: 2.8; 95% CI: 1.2-6.3). Having malaria was only a predictor of ACT purchase in the region with a drug shop accreditation program (aOR: 3.4; 95% CI: 1.5-7.4).\ud Malaria is common amongst persons presenting to drug shops with a complaint of fever. The low proportion of persons with malaria purchasing ACTs, and the high proportion of ACTs going to persons without malaria demonstrates a need to better target who receives ACTs in these drug shops

Bioassay-guided investigation of the Tanzanian plant Pyrenacantha kaurabassana for potential anti-HIV-active compounds

Two new anti-HIV xanthones, 6,7,11-trihydroxy-10-methoxy-9-(7-methoxy-3-methyl-1-oxoisochroman-5-yl)-2-methyl- 12-oxo-12H-benzo[b]xanthene-4-carboxylic acid (1) and 6,7-dihydroxy-10,11-dimethoxy-9- (7-methoxy-3-methyl-1-oxoisochroman-5-yl)-2-methyl-12-oxo-12H-benzo [b]xanthene-4-carboxylic acid (2), and a new hexadecahydrochrysen-3-ol (3) were isolated from the tubers of Pyrenacantha kaurabassana. Compounds 1 and 2 showed moderate anti-HIV activity when tested in the deCIPhR assay on HIV virus type NL4-3, with IC50 values of 21 and 2 mu g/mL, respectively

Screening of traditionally used Tanzanian medicinal plants for antifungal activity

Kisangau DP, Hosea KM, Lyaruu HVM, et al. Screening of traditionally used Tanzanian medicinal plants for antifungal activity. PHARMACEUTICAL BIOLOGY. 2009;47(8):708-716.Fungal infections represent a significant cause of morbidity and mortality especially in immunocompromised patients in the world today. Dichloromethane (DM) and aqueous (W) extracts of nine plants used traditionally for the treatment of fungal infections in Bukoba rural district in Tanzania were screened for antifungal activity against Candida albicans, Cryptococcus neoformans, and Aspergillus niger using agar well and disk diffusion methods. Dichloromethane extracts of Capparis erythrocorpos [CE] Isert (Capparaceae), Cussonia arborea [CA] Hochst. Ex A. Rich (Araliaceae), Drocaena steudneri [DS] Engl. (Dracaenaceae), Lannea schimperi [LS] (A. Rich) Engl. (Anacardiaceae), Rouvolfia vomitoria [RV] Afz (Apocynaceae), and Sapium ellipticum [SE] (Krauss) Pax (Euphorbiaceae) showed activity against all three fungi. Extracts of Rumex usambarensis [RU] (Dammer) Dammer (Polygonaceae) and Zehneria scabro [ZS] (L.f.) Sond. (Cucurbitaceae) had an activity limited to only one or two of the test organisms. Rhoicissus tridentata [RT] (L.Q Wild & Drum (Vitaceae) was the only plant without activity. Fractions of the active extracts CE, CA, DS, LS, and SE exhibited higher antifungal activity against one or more of the three fungi. Four compounds isolated from S. ellipticum also exhibited antifungal activity against one or more of the three fungi. The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs), determined using the microplate assay method, ranged between 0.4 and 50.0 mu g/mL for crude extracts, 1.6 and 50.0 mu g/mL for semi-purified fractions, and 0.12 and 1.0 mu g/mL for pure compounds, as compared to 0.016-1.5 mu g/mL for fluconazole. We confirm the potential of traditionally used plants as a source of new drugs for treatment of fungal infections