Über einen energieunabhängigen Me2+-H+-Austausch in der Auβenmembran von Rattenlebermitochondrien und seine Beziehungen zum energieunabhängigen Me+-H+-Austausch

AbstractIntact mitochondria are able to bind monovalent and divalent metal cations and to release protons in an energy-independent exchange process. Directly accessible binding sites exist in the outer membrane. They seem to be identical for monovalent and divalent metal ions. The inner membrane-matrix-fraction possesses exchange sites after ultrasonic disruption only for monovalent cations, but not for divalent cations

Über einen energieunabhängigen austausch der alkali-ionen Li+, Na+, K+, Rb+ und Cs+ GEGEN H+ in der membran intakter und desintegrierter rattenlebermitochondrien

Abstract1.1. The energy-independent exchange of alkali metal cations against protons was investigated in intact and disintegrated mitochondria. The exchange is not specific for different alkali metal ions.2.2. In intact mitochondria the apparent Km for the energy-independent exchange range from 5 to 25 mM (Cs+, Rb+, K+ 5–10 mM; Na+ 15 mM; Li+ 20 mM). At all ions studied the maximum release of protons was 15–20 nval/mg protein.3.3. In disintegrated mitochondria there are no differences between the alkali metal ions (apparent Km 30 mM; maximum H+-release 50 nval/mg protein).4.4. Directly accessible binding sites seem to exist in the outer membrane. Binding sites in the inner membrane are accessible only after disintegration, but not after addition of valinomycin + rotenone

Human papilloma virus is associated with breast cancer

Background: There is increasing evidence that high-risk human papilloma virus (HPV) is involved in cancers in addition to cervical cancer. For example, it is generally accepted that HPV has a role in a significant proportion of head and neck tumours, and it has long been hypothesised that hormone dependent oncogenic viruses, such as HPV may have causal roles in some human breast cancers. A number of reports have identified HPV DNA in breast tissue and breast cancer specimens, but these rely on standard polymerase chain reaction (PCR), which is criticised for its propensity for contamination. Methods: We have used two different technologies, in situ and standard PCR (with sequencing), and histology based on light microscopy. Results: We unambiguously demonstrate the presence of high-risk HPV in the cells of breast cancer specimens and breast cancer cell lines. In addition, we also show that the oncogenic characteristics of HPV associated breast cancer are very similar to HPV-associated cervical cancer. Specifically, that putative koilocytes are present in some HPV associated breast cancers. Interpretation: The above observations indicate a likely causal role for high-risk HPV in human breast cancer and offer the possibility of primary prevention of some breast cancers by vaccination against HPV.6 page(s

Final Report - Effects of High Spinel and Chromium Oxide Crystal Contents on Simulated HLW Vitrification in DM100 Melter Tests, VSL-09R1520-1, Rev. 0, dated 6/22/09

The principal objective of the work was to evaluate the effects of spinel and chromium oxide particles on WTP HLW melter operations and potential impacts on melter life. This was accomplished through a combination of crucible-scale tests, settling and rheological tests, and tests on the DM100 melter system. Crucible testing was designed to develop and identify HLW glass compositions with high waste loadings that exhibit formation of crystalline spinel and/or chromium oxide phases up to relatively high crystal contents (i.e., > 1 vol%). Characterization of crystal settling and the effects on melt rheology was performed on the HLW glass formulations. Appropriate candidate HLW glass formulations were selected, based on characterization results, to support subsequent melter tests. In the present work, crucible melts were formulated that exhibit up to about 4.4 vol% crystallization

Sur les solutions périodiques du mouvement plan de libration des satellites et des planètes

Ce papier présente une étude analytique du mouvement plan de rotation des satellites (et des planètes) dans leurs mouvements orbitaux. Les trois familles des solution périodiques sont obtenues par la méthode du prolongement analytique de Poincaré. Ensuite, la stabilité de ces solutions périodiques est discutée, et les équations approchées des courbes limites de stabilité sont données jusqu'au quatrième ordre. This paper presents an analytical study of the rotational motion of the satellites (and the planets) in their orbital planes. The three families of periodic solutions are obtained by the method of analytical continuation as formulated by Poincaré. The stability of these solutions are analyzed, and the approximate equations of the transition curves are obtained to the fourth order.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42563/1/10569_2005_Article_BF01227807.pd