Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair

Characterization of hMSCs and hMSC-chondrogenic pellets. (a) Flow cytometric analysis of the hematopoietic markers (CD34, CD45) and MSC markers (CD73, CD105) in hMSCs. The proportion of CD73-expressing cells was 99.7 ± 0.63 %; The proportion of CD105-expressing cells was 98.7 ± 1.21 %. Values represent means ± SEM; n = 3. (b) Three-dimensional pellet culture of the hMSC-chondrogenic pellet. (c) Alcian blue staining and (d) toluidine blue staining of glycosaminoglycans and proteoglycans revealed the chondrocyte-type appearance in the hMSC-chondrogenic pellets. Scale bar = 100 μm. (e,f) Immunohistochemistry for type II and type X collagen. Scale bar = 100 μm. (TIF 8130 kb

Effects of organic plant oils and role of oxidation on nutrient utilization in juvenile rainbow trout (Oncorhynchus mykiss)

The study compared the effect of four either fresh or force oxidized organic plant oils in diets for juvenile rainbow trout (Oncorhynchus mykiss) in which 47% of conventional LT fish meal protein was substituted by a mixture of 3 organic plant protein concentrates. Fish oil was completely substituted with either organic linseed oil; rape seed oil; sunflower oil or grape seed oil and evaluated based on feed intake, feed utilization, growth and digestibility. None of the plant oils affected feed intake and growth parameters. Organic plant oils had all a positive effect on lipid digestibility as compared with the fish oil based control diet, despite the very different FA profiles. The organic vegetable oils did not undergo autoxidation, as opposed to the fish oil control for which lipid digestibility was significantly negative influenced

X-Ray Diffuse Scattering

Contains research summary and reports on two research projects.Joint Services Electronics Program (Contract DAAL03-86-K-0002)Joint Services Electronics Program (Contract DAAL03-89-C-0001

Recent topics of pharmacology in oriental medicine : detoxification of morphine addiction in the mouse narcotic dependence model using extracts from psidium guajava leaves

The effects of a polar fraction of an extract from the leaf of the guava plant, Psidium guajava Linn (Myrtaceae), including the effects of two isolated flavonoid compounds, quercetin and quercetin-3-arabinoside, on withdrawal symptoms in morphine-dependent mouse models were studied. Two types of mouse narcotic-dependence models were employed.Mouse chronic narcotic-dependence model was prepared by thrice daily 1.p injection of morphine sulphate, with an initial dose of 4 mg/kg and increasing each successive dose by 4 mg/kg, up to day-9, when the dose of 108 mg/kg reached was maintained through day-11 and the morning of day-12.Mouse acutely narcotic-dependence model was prepared by a s.c. bolus injection of 50 mg/kg morphine sulphate, followed 4 h later by s.c. injection of 10 mg/kg naloxone. Manifestations of abstinence syndrome, characterised by repetitive vertical jumping,rearing,paw-shaking,head-shaking,teeth chattering,etc.,occurred about 14 h after the last dose in group A,and 45 - 60 min after naloxone injection in group B.A s.c. dose of 0.5 mg/kg of the extract, 10 h after the last dose in group A or 1 h before the naloxone injection in group B, prevented the repeated vertical jumps and greatly reduced the other symptoms of withdrawal.Parallel experiments on satiety to the extract in mice showed no development of dependence on the extract. Quercetin may be the main active compound in the extract

X-Ray Diffuse Scattering

Contains introduction and reports on three research projects.Joint Services Electronics Program DAAL03-89-C-000

On convergence of (

A generalization of the classical Montessus de Ballore theorem in ℂn and some related theorems are discussed. Non-Montessus type of convergence in ℝ2n dimensional Lebesgue measure is presented linking it to a result of Gonĉar