Metabolismo de ureidos y nucleótidos durante la germinación y desarrollo inicial de plántulas de Phaseolus vulgaris

La judía (Phasealus vulgaris) es una leguminosa que transporta la mayoría del nitrógeno fijado en los nódulos hacia las partes aéreas en forma de ureidos. Estos compuestos tienen una elevada relación N/e, lo que los convierte en moléculas ideales de transporte y almacenamiento de nitrógeno. La germinación y desarrollo postgerminativo son dos etapas cruciales en el ciclo de vida de las plantas. En estos periodos, las plantas son heterotróficas ya que dependen completamente de las reservas acumuladas en las semillas. Antes de que los nutrientes se agoten, las plántulas pasan a un metabolismo autotrófico. En este trabajo se ha estudiado el metabolismo de ureidos y nucleótidos en plántulas de judía durante la germinación y desarrollo postgerminativo temprano. Las semillas de judía presentan niveles muy altos de ureidos además de elevados valores de actividad alantoinasa. Tras la emergencia radicular, se produce una inducción del metabolismo de ureidos principalmente en los ejes en desarrollo. Las actividades enzimáticas implicadas en la degradación de los ureidos alantoína, alantoato y ureidoglicolato se inducen en ejes en desarrollo, lo que sugiere un papel importante de estos compuestos en la movilización del nitrógeno durante la germinación y desarrollo postgerminativo. Se han obtenido anticuerpos frente a la proteína alantoinasa sobreexpresada en E. coli, que reconocen al menos tres proteínas en extractos de judía. En la ruta de síntesis de ureidos a partir de purinas, el primer paso estaría catalizado por una actividad fosfatasa que catalizaría la conversión de nucleótidos a nucleósidos. Se ha determinado y caracterizado una actividad fosfatasa resistente a molibdato, inhibidor de fosfatasas ácidas, en ejes de judía y cuyo patrón temporal de actividad correlaciona con el aumento de ureidos. Se determinó actividad tanto con nucleótidos purínicos como pirimidínicos. Se ha clonado un AONc que codifica una nucleotidasa con dominios similares a los de una nucleotidasa específica de pirimidinas. Sin embargo, la proteína sobreexpresada presentó actividad tanto con pirimidinas como purinas.French bean (Phaseolus vulgaris) is a legume that transports most of the fixed nitrogen from the nodules to the aerial parts as ureides. These compounds have a high N/e ratio, which makes them ideal for the transport and storage of nitrogen. Germination and seedlings growth are two crucial stages in the life cycle of plants. In these periods, plants are heterotrophic and they dependent on the reserves accumulated in the seeds. The plants become autotrophic before the nutrients are depleted. In this work, we have studied nucleotide and ureide metabolism in French bean during germination and seedlings development. Oried common bean seeds accumulate ureides and they show high levels of allantoinase activity. After radicle protusion, ureide metabolism is induced mainly in developing axes. The enzymatic activities involved in the degradation of ureides (allantoin, alantoate, and ureidoglicolate degrading activities) are induced in developing axes, suggesting a role of these compounds in the mobilization of nitrogen during germination and seedling growth. Antibodies against the common bean allantoinase were raised by using purified protein overexpressed in Escherichia cali and they recognize at least three proteins in crude extracts from common bean. The first step in the pathway from purines to ureides is catalysed by a phosphatase, which transforms nucleotides to nucleosides. A molibdate resistant phosphatase activity has been determined and characterized from developing axes. The values of phosphatase activity during germination and seedling development correlates with the ureide accumulation pattern. Molibdate-resistant phosphatase was determined with both purine and pyrimidine nucleotides as substrates. A cONA encoding a putative pyrimidine nucleotidase was cloned from French bean. However, the overexpressed protein showed activity with both pyrimidines and purines as substrates

Biotecnología de plantas superiores y algas verdes

Los ureidos, alantoína y alantoato, son los principales compuestos sintetizados a partir del nitrógeno fijado en los nódulos de las leguminosas que se exportan a las partes aéreas en leguminosas tropicales como la judía. Los ureidos se producen por la oxidación de las purinas sintetizadas de novo en los nódulos radicales y también como parte de un proceso de recuperación de los compuestos nitrogenados en tejidos senescentes. Los ureidos se acumulan en varios tejidos vegetales en respuesta al estrés hídrico, y se ha sugerido que la acumulación de estos compuestos nitrogenados es la responsable de la inhibición de la fijación de nitrógeno que tiene lugar en condiciones ambientales adversas. A pesar de la importancia crucial de los ureidos como compuestos de reserva y transporte de nitrógeno, hasta el momento no se llevado a cabo la caracterización de las rutas de síntesis y degradación de ureidos en plantas. En particular, parece que existen dos posibles rutas de degradación de alantoato, y que el que determinadas leguminosas usen una u otra ruta puede afectar a la sensibilidad o tolerancia de esas plantas a la sequía. Por tanto la determinación de la ruta que opera en leguminosas de gran interés agronómico como la judía y el garbanzo, puede a la larga tener utilidad biotecnológica con la obtención de plantas que sean capaces de mantener la fijación de nitrógeno en condiciones ambientales adversas, como la sequía. Además de en la caracterización, con fines biotecnológicos del metabolismo de ureidos en leguminosas, se está trabajando obtención de algas superproductoras de γ-tocoferol (vitamina E) mediante ingeniería metabólica, así como en el análisis del contenido en antioxidantes del aceite de oliv

Evolution of the use of corticosteroids for the treatment of hospitalised COVID-19 patients in Spain between March and November 2020: SEMI-COVID national registry

Objectives: Since the results of the RECOVERY trial, WHO recommendations about the use of corticosteroids (CTs) in COVID-19 have changed. The aim of the study is to analyse the evolutive use of CTs in Spain during the pandemic to assess the potential influence of new recommendations. Material and methods: A retrospective, descriptive, and observational study was conducted on adults hospitalised due to COVID-19 in Spain who were included in the SEMI-COVID- 19 Registry from March to November 2020. Results: CTs were used in 6053 (36.21%) of the included patients. The patients were older (mean (SD)) (69.6 (14.6) vs. 66.0 (16.8) years; p < 0.001), with hypertension (57.0% vs. 47.7%; p < 0.001), obesity (26.4% vs. 19.3%; p < 0.0001), and multimorbidity prevalence (20.6% vs. 16.1%; p < 0.001). These patients had higher values (mean (95% CI)) of C-reactive protein (CRP) (86 (32.7-160) vs. 49.3 (16-109) mg/dL; p < 0.001), ferritin (791 (393-1534) vs. 470 (236- 996) µg/dL; p < 0.001), D dimer (750 (430-1400) vs. 617 (345-1180) µg/dL; p < 0.001), and lower Sp02/Fi02 (266 (91.1) vs. 301 (101); p < 0.001). Since June 2020, there was an increment in the use of CTs (March vs. September; p < 0.001). Overall, 20% did not receive steroids, and 40% received less than 200 mg accumulated prednisone equivalent dose (APED). Severe patients are treated with higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%. Conclusions: Patients with greater comorbidity, severity, and inflammatory markers were those treated with CTs. In severe patients, there is a trend towards the use of higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%

7th Drug hypersensitivity meeting: part two

No abstract availabl

A NuSTAR view of SS433: Precessional evolution of the jet-disk system

SS433 is a Galactic microquasar with powerful outflows (double jet, accretion disk and winds) with well known orbital, precessional and nutational period. In this work we characterise different outflow parameters throughout the precessional cycle of the system. We analyse 10 NuSTAR ($3-70$ keV) observations of $\sim$30~ks that span $\sim$1.5 precessional cycles. We extract averaged spectra and model them using a combination of a double thermal jet model (bjet) and pure neutral and relativistic reflection (xillverCp and relxilllpCp) over an accretion disk. We find an average jet bulk velocity of $\beta = v/c \sim0.29$ with an opening angle of $\lesssim$6~degrees. Eastern jet kinetic power ranges from 1 to $10^{39}$~erg/s, with base "coronal" temperatures $T_o$ ranging between 14 and 18 keV. Nickel to iron abundances remain constant at $\sim$9 (within 1$\sigma$). The western to eastern jet flux ratio becomes $\sim1$ on intermediate phases, about 35% of the total precessional orbit. The $3-70$ keV total unabsorbed luminosity of the jet and disk ranges from 2 to 20 $\times$10$^{37}$~erg/s, with the disk reflection component contributing mainly to the hard $20-30$ keV excess and the stationary 6.7 keV ionized Fe line complex. At low opening angles $\Theta$ we find that the jet expands sideways following an adiabatic expansion of a gas with temperature $T_o$. Finally, the central source and lower parts of the jet could be hidden by an optically thick region of $\tau > 0.1$ and size $R\sim N_H/n_{e0}\sim1.5\times10^9$~cm$\sim$1700~$r_g$ for $M_{BH}=3~M_{\odot}

A NuSTAR view of SS433: Precessional evolution of the jet-disk system

SS433 is a Galactic microquasar with powerful outflows (double jet, accretion disk and winds) with well known orbital, precessional and nutational period. In this work we characterise different outflow parameters throughout the precessional cycle of the system. We analyse 10 NuSTAR ($3-70$ keV) observations of $\sim$30~ks that span $\sim$1.5 precessional cycles. We extract averaged spectra and model them using a combination of a double thermal jet model (bjet) and pure neutral and relativistic reflection (xillverCp and relxilllpCp) over an accretion disk. We find an average jet bulk velocity of $\beta = v/c \sim0.29$ with an opening angle of $\lesssim$6~degrees. Eastern jet kinetic power ranges from 1 to $10^{39}$~erg/s, with base "coronal" temperatures $T_o$ ranging between 14 and 18 keV. Nickel to iron abundances remain constant at $\sim$9 (within 1$\sigma$). The western to eastern jet flux ratio becomes $\sim1$ on intermediate phases, about 35% of the total precessional orbit. The $3-70$ keV total unabsorbed luminosity of the jet and disk ranges from 2 to 20 $\times$10$^{37}$~erg/s, with the disk reflection component contributing mainly to the hard $20-30$ keV excess and the stationary 6.7 keV ionized Fe line complex. At low opening angles $\Theta$ we find that the jet expands sideways following an adiabatic expansion of a gas with temperature $T_o$. Finally, the central source and lower parts of the jet could be hidden by an optically thick region of $\tau > 0.1$ and size $R\sim N_H/n_{e0}\sim1.5\times10^9$~cm$\sim$1700~$r_g$ for $M_{BH}=3~M_{\odot}

A NuSTAR view of SS433: Precessional evolution of the jet-disk system

SS433 is a Galactic microquasar with powerful outflows (double jet, accretion disk and winds) with well known orbital, precessional and nutational period. In this work we characterise different outflow parameters throughout the precessional cycle of the system. We analyse 10 NuSTAR ($3-70$ keV) observations of $\sim$30~ks that span $\sim$1.5 precessional cycles. We extract averaged spectra and model them using a combination of a double thermal jet model (bjet) and pure neutral and relativistic reflection (xillverCp and relxilllpCp) over an accretion disk. We find an average jet bulk velocity of $\beta = v/c \sim0.29$ with an opening angle of $\lesssim$6~degrees. Eastern jet kinetic power ranges from 1 to $10^{39}$~erg/s, with base "coronal" temperatures $T_o$ ranging between 14 and 18 keV. Nickel to iron abundances remain constant at $\sim$9 (within 1$\sigma$). The western to eastern jet flux ratio becomes $\sim1$ on intermediate phases, about 35% of the total precessional orbit. The $3-70$ keV total unabsorbed luminosity of the jet and disk ranges from 2 to 20 $\times$10$^{37}$~erg/s, with the disk reflection component contributing mainly to the hard $20-30$ keV excess and the stationary 6.7 keV ionized Fe line complex. At low opening angles $\Theta$ we find that the jet expands sideways following an adiabatic expansion of a gas with temperature $T_o$. Finally, the central source and lower parts of the jet could be hidden by an optically thick region of $\tau > 0.1$ and size $R\sim N_H/n_{e0}\sim1.5\times10^9$~cm$\sim$1700~$r_g$ for $M_{BH}=3~M_{\odot}

PDCD1 polymorphisms are not associated with Takayasu's arteritis in Turkey

PubMedID: 22274654Objectives: Takayasu's arteritis (TA) is a chronic arterial inflammation of unknown etiology involving mainly the aorta and its major branches. Based on the associations of programmed death-1 (PD-1) protein encoding gene (PDCD1) with connective tissue diseases and vasculitides, PDCD1 polymorphisms are studied for susceptibility to TA in this study. Methods: The study group is made up of TA patients (n=229) fulfilling the 1990 ACR classification criteria and compared to 193 healthy controls (HC). PD-1.3, PD-1.5 and PD-1.6 single nucleotide polymorphisms of PDCD1 gene are genotyped by polymerase chain reaction and restriction analysis (PCR-RFLP). Results: The distribution of PD-1.5 polymorphism in TA patients and HC revealed a similar presence of TT genotype in patients and controls (13.3% vs. 11.4%). PD-1.3 and PD-1.6 were less polymorphic and did not differ between the groups. Rare AA genotype of PD-1.3 (1.4% vs. 1.0%) and AG genotype of PD-1.6 was again similarly (22.4% vs. 19.2%) present in TA and HC. Conclusion: PD-1.3, 1.5 and 1.6 polymorphisms of PDCD1 gene, which were shown to be associated with various autoimmune disorders and vasculitides, are not associated with a susceptibility to TA in Turkish population. © Clinical and Experimental Rheumatology 2012