Rezensionen

PESCHEL, CORINNA /RUNSCHKE, KERSTIN (eds.) (2015): Sprachvariation und Sprachreflexion in interkulturellen Kontexten. (= Sprache – Kommunikation – Kultur, Soziolinguistische Beiträge 16) Frankfurta. M.: Peter Lang. 394 S. SATA, LEHEL (2016): Paradox und mystische Sinnlichkeit – Angelus Silesius’ „Cherubinischer Wandersmann“ im Lichte der Theosophie und Sprachphilosophie Jakob Böhmes (Poetica, 140). Hamburg: Dr. Kovač. 303 S. MATTHIES, ANNEMARIE (2016): Spielbälle. Neuverhandlungen der Arbeitswelt im Medium der Literatur. Konstanz: UVK. 328 S. SOMMERFELD, BEATE (2016): Übersetzungskritik. Modelle, Perspektiven, Didaktik. Poznań: Wydawnictwo Naukowe UAM. 138 S. WEIDNER, DANIEL (ed.) (2016): Handbuch. Literatur und Religion. Stuttgart: J.B. Metzler Verlag. 484 S. HELBIG-MISCHEWSKI, BRIGITTA / ZDUNIAK-WIKTOROWICZ, MAŁGORZATA (eds.) (2016): Migrantenliteratur im Wandel. Junge Prosa mit (nicht nur) polnischen Wurzeln in Deutschland und Europa / Literatura migracyjna w procesie. Młoda proza (nie tylko) polskiego pochodzenia w Niemczech i Europie (= Studien zum deutsch-polnischen Kulturtransfer, Band 6). Leipzig: Leipziger Universitätsverlag. 294 S. SOMMERFELD, BEATE / KĘSICKA, KAROLINA / KORYCIŃSKA-WEGNER, MAŁGORZATA / FIMIAK-CHWIŁKOWSKA, ANNA (EDS.) (2017): Über- setzungskritisches Handeln. Modelle und Fallstudien (= Studien zur Germanistik, Skandinavistik und Übersetzungskultur 16). Frankfurt a. M.: Peter Lang. 238 S

Migration-promoting factors in the intestinal T-cell homing during acute graft-versus-host disease

Die akute Graft-versus-Host Erkrankung (GvHD), insbesondere die Darm GvHD, stellt weiterhin eine der Hauptursachen für Mortalität und Morbidität nach allogener SZT dar. Aktivierte, alloreaktive Spender T-Zellen infiltrieren dabei über die Blutbahn die intestinale Lamina Propria. Erst kürzlich konnten wir zeigen, dass neben der vaskulären Migration ein Teil der Spender T-Zellen auch direkt aus den PP in die angrenzende Lamina Propria migrieren. Um Faktoren, die diese direkte Migration fördern, zu untersuchen und die direkt migrierenden T-Zellen genauer zu charakterisieren, verwendeten wir ein MHC-inkompatibles Mausmodell zur Induktion einer akuten GvHD. Durch RNA Sequenzierung und Massenspektrometrie lasermikrodissezierter Darmschleimhautproben konnte eine starke Expression der Chemokine CXCL9, CXCL10, CXCL11, CCL3, CCL4 und CCL5 während der akuten intestinalen GvHD aufgezeigt werden. Neben CCL4 und XCL1 wiesen verschiedene Faktoren der T-Zellaktivierung, wie CD3ζ, LAT, Lck und ZAP70, sowie Faktoren der zytoskelettalen Reorganisation, wie Dock2, Coro1α und Parvin-γ, eine vermehrte Expression insbesondere nahe der PP auf. Die Expression der migrationsfördernden Faktoren Coro1α und Parvin-γ in Spender T-Zellen nahe der PP konnte anschließend mittels histologischen Immunfluoreszenzfärbungen bestätigt werden. Durchflusszytometrische Analysen konnten weiterhin eine vermehrte Expression von CCR5, CCR9 und Intgerin α4β7 auf den vornehmlich Tbet+ Spender T-Zellen nahe der PP nachweisen. Funktionelle in vitro Migrationsversuche zeigten abschließend, dass in vivo aktivierte Spender T-Zellen eine gerichtete Migration in Richtung auf CXCL11 und zu späterem Zeitpunkt auch auf CCL4 vollziehen können. Zusammenfassend zeigt diese Arbeit die Bedeutung zahlreicher Chemokine für das sequenzielle T-Zell-Homing während der akuten intestinalen GvHD. Neben der insbesondere durch Faktoren der zytosekeletalen Reorganisation vermittelten amoeboiden Migration kann auch eine mesenchymale Fortbewegung über Faktoren wie CCR5, CCR9 und Integrin α4β7 die direkte Migration der T-Zellen fördern. Den direkt migrierenden vornehmlich TH1 polarisierten Zellen folgen weitere, CD27 und Integrin αLβ2 exprimierende, zytotoxische T-Zellen aus der Blutbahn. Die direkt migrierenden Zellen könnten als Initiator und Potentiator der intestinalen T-Zell Infiltration wirken und müssen für zukünftige therapeutische Strategien nicht nur der Darm GvHD, sondern der intestinalen Inflammation im Allgemeinen mitberücksichtigt werden.Acute graft-verus-host disease (GvHD), especially intestinal GvHD, remains one of the main causes of mortality and morbidity after allogeneic hematopoietic stem cell transplantation. In this process activated alloreactive donor T cells infiltrate the intestinal lamina propria via the bloodstream. Our group could recently show that besides the vascular migration route some donor T cells migrate directly from the Peyer’s patches into the adjacent lamina propria. To investigate factors that could promote such a direct migration, and to characterize these direct migrating T cells we applied a major mismatch mouse model to induce acute GvHD. Using RNA sequencing and mass spectrometry of lasermicrodissected lamina propria samples, we detected a strong upregulation of the chemokines CXCL9, CXCL10, CXCL11, CCL3, CCL4 and CCL5 during acute intestinal GvHD. Alongside CCL4 and XCL1, several factors of T cell activation, such as CD3ζ, LAT, Lck und ZAP70, as well as factors of cytoskeletal reorganization, such as Dock2, Coro1α und Parvin-γ, showed higher expression near the Peyer’s patches. Subsequently, we validated the expression of Coro1α and Parvin-γ on donor T cells near the Peyer’s patches with histological immunofluorescence stainings. Flow cytometry analysis further revealed high expression of CCR5, CCR9 and Intgerin α4β7 on the predominantly Tbet+ donor T cells near the Peyer’s patches. Conclusively, in vitro migration assays showed that in vivo activated donor T cells can directly migrate towards CXCL11 and subsequently also towards CCL4. The present study shows the relevance of several chemokines for the sequential T-cell homing during acute intestinal GvHD. Besides the amoeboid migration mode, which is particularly driven by cytoskeletal reorganization, a mesenchymal movement using factors, such as CCR5, CCR9 and Integrin α4β7, can promote the direct migration of donor T cells. The directly migrating cells, which are predominantly of a TH1 phenotype, are followed by cytotoxic T cells, expressing CD27 and Integrin αLβ2 (LFA-1), from the systemic circulation. Thus, these directly migrating cells may act like an initiator and potentiator for the intestinal T cell infiltration and must be considered for new therapeutic strategies not only of GvHD but of intestinal inflammation in genera

How to insert visual information into a whiteboard animation with a human hand? Effects of different insertion styles on learning

Abstract Whiteboard animations have become very popular in recent years. They are mainly used in distance education, where learners can acquire knowledge individually and without the help of a teacher. However, there is little empirical evidence on how whiteboard animations should be designed to achieve learning-enhancing effects. Since the presentation of whiteboard animations is reminiscent of a teacher drawing or showing content on a whiteboard, the hand has been identified as an essential feature of this learning medium. Therefore, the aim of this experimental study was to investigate whether and how the human hand should be implemented in whiteboard animations for the presentation of visual content. University students (N = 84) watched a whiteboard animation in which the type of information insertion was manipulated (hand drawing content vs. hand pushing content in vs. no hand visible). Results revealed that the drawing hand on a whiteboard led to significantly higher intrinsic motivation than the hand pushing visual content onto the whiteboard. Contrary to assumptions derived from cognitive load theory, the implementation of a human hand did not cause extraneous cognitive load. However, no other effects on the perception of the instructor, cognitive load, and learning performance were found. The results are discussed in terms of both cognitive and social processes in multimedia learning

Exploring Fonts as Retrieval Cues in Text-Based Learning

The purpose of this experiment was to determine whether the font can serve as a retrieval cue in text-based learning. Hereby, the experiment was unique in that both the learning texts and learning questions were manipulated for font. This involved reusing the same font (Haettenschweiler and Times New Roman) for the learning texts and for the learning questions. Based on the context-dependent memory effect (Smith & Vela, 2001) and empirical findings on retrieval cues (e.g., Grant et al., 1998; Schneider et al., 2020; Skulmowski & Rey, 2021), it was hypothesized that using the same font for both learning and recall would result in better learning performance (Godden & Baddeley, 1975)

18F-FDG, 11C-methionine, and 68Ga-pentixafor PET/CT in patients with smoldering multiple myeloma: imaging pattern and clinical features

This study aimed to explore the correlation between imaging patterns and clinical features in patients with smoldering multiple myeloma (SMM) who simultaneously underwent 18F-FDG, 11C-Methionine, and 68Ga-Pentixafor positron emission tomography/computed tomography (PET/CT). We retrieved and analyzed clinical characteristics and PET imaging data of 10 patients with SMM. We found a significant correlation between bone marrow (BM) plasma cell (PC) infiltration and mean standardized uptake values (SUV$_{mean}$) of lumbar vertebrae L2-L4 on 11C-Methionine PET/CT scans (r = 0.676, p = 0.031) and 68Ga-Pentixafor PET/CT scans (r = 0.839, p = 0.002). However, there was no significant correlation between BM involvement and SUV$_{mean}$ of lumbar vertebrae L2-L4 on 18F-FDG PET/CT scans (r = 0.558, p = 0.093). Similarly, mean target-to-background ratios (TBR$_{mean}$) of lumbar vertebrae L2-L4 also correlated with bone marrow plasma cell (BMPC) infiltration in 11C-Methionine PET/CT (r = 0.789, p = 0.007) and 68Ga-Pentixafor PET/CT (r = 0.724, p = 0.018) PET/CT. In contrast, we did not observe a significant correlation between BMPC infiltration rate and TBR$_{mean}$ in 18F-FDG PET/CT (r = 0.355, p = 0.313). Additionally, on 11C-Methionine PET/CT scans, we found a significant correlation between BMPC infiltration and TBR$_{max}$ of lumbar vertebrae L2-L4 (r = 0.642, p = 0.045). In conclusion, 11C-Methionine and 68Ga-Pentixafor PET/CT demonstrate higher sensitivity than 18F-FDG PET/CT in detecting BM involvement in SMM

The link between cytogenetics/genomics and imaging patterns of relapse and progression in patients with relapsed/refractory multiple myeloma: a pilot study utilizing 18F-FDG PET/CT

Utilizing 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET)/computed tomography (CT), we performed this pilot study to evaluate the link between cytogenetic/genomic markers and imaging patterns in relapsed/refractory (RR) multiple myeloma (MM). We retrospectively analyzed data of 24 patients with RRMM who were treated at our institution between November 2018 and February 2020. At the last relapse/progression, patients had been treated with a median of three (range 1–10) lines of therapy. Six (25%) patients showed FDG avid extramedullary disease without adjacency to bone. We observed significantly higher maximum standardized uptake values (SUVmax) in patients harboring del(17p) compared with those without del(17p) (p = 0.025). Moreover, a high SUVmax of >15 indicated significantly shortened progression-free survival (PFS) (p = 0.01) and overall survival (OS) (p = 0.0002). One female patient exhibited biallelic TP53 alteration, i.e., deletion and mutation, in whom an extremely high SUVmax of 37.88 was observed. In summary, this pilot study suggested a link between del(17p)/TP53 alteration and high SUVmax on 18F-FDG PET/CT in RRMM patients. Further investigations are highly warranted at this point

ICOS immunoPET enables visualization of activated T cells and early diagnosis of murine acute gastrointestinal GvHD

Allogeneic hematopoietic cell transplantation (HCT) is a well-established and potentially curative treatment for a broad range of hematological diseases, bone marrow failure states, and genetic disorders. Acute graft-versus-host disease (GvHD), mediated by donor T cells attacking host tissues, still represents a major cause of morbidity and mortality following allogeneic HCT. Current approaches to diagnosis of gastrointestinal acute GvHD rely on clinical and pathological criteria that manifest at late stages of disease. New strategies allowing for GvHD prediction and diagnosis, prior to symptom onset, are urgently needed. Noninvasive antibody-based positron emission tomography (PET) (immunoPET) imaging of T-cell activation post-allogeneic HCT is a promising strategy toward this goal. In this work, we identified inducible T-cell costimulator (ICOS) as a potential immunoPET target for imaging activated T cells during GvHD. We demonstrate that the use of the Zirconium-89-deferoxamine-ICOS monoclonal antibody PET tracer allows in vivo visualization of donor T-cell activation in target tissues, namely the intestinal tract, in a murine model of acute GvHD. Importantly, we demonstrate that the Zirconium-89-deferoxamine-ICOS monoclonal antibody PET tracer does not affect GvHD pathogenesis or the graft-versus-tumor (GvT) effect of the transplant procedure. Our data identify ICOS immunoPET as a promising strategy for early GvHD diagnosis prior to the appearance of clinical symptoms