Anticholinergics: A potential option for preventing posttraumatic epilepsy

Interest in the use of anticholinergics to prevent the development of epilepsy after traumatic brain injury (TBI) has grown since recent basic studies have shown their effectiveness in modifying the epileptogenic process. These studies demonstrated that treatment with anticholinergics, in the acute phase after brain injury, decreases seizure frequency, and severity, and the number of spontaneous recurrent seizures (SRS). Therefore, anticholinergics may reduce the risk of developing posttraumatic epilepsy (PTE). In this brief review, we summarize the role of the cholinergic system in epilepsy and the key findings from using anticholinergic drugs to prevent PTE in animal models and new clinical trial protocols. Furthermore, we discuss why treatment with anticholinergics is more likely to prevent PTE than treatment for other epilepsies

Crescimento inicial de clones de palma forrageira submetidos a níveis de adubação potássica

Objetivou-se avaliar o efeito de diferentes níveis de adubação potássica no crescimento inicial de clones de palma forrageira (Nopalea cochenillifera Salm Dyck). O experimento foi conduzido durante seis meses, disposto em delineamento em blocos ao acaso, em esquema fatorial 5x2, sendo cinco níveis de adubação potássica (0, 250, 500, 750 e 1000 Kg de K2O ha-1) e dois clones de palma forrageira (Doce Gigante e Doce Miúda), com quatro repetições. Foi plantado um cladódio por vaso (unidade experimental) com capacidade para 7 kg de solo (24 x 17 x 23 cm). Ao longo do período experimental, foram avaliadas as características estruturais das plantas, tais como altura e largura de planta, comprimento, largura, espessura e perímetro de cladódio, bem como contabilização do número de cladódios total e por ordem de surgimento. Ao final do experimento avaliou-se a biomassa, para determinação do rendimento de massa fresca (MV) e seca (MS) da parte aérea e do sistema radicular. Após a determinação de MS, determinou-se o teor potássico nos tecidos vegetais. Os dados foram submetidos a ANOVA e, caso necessário, ao teste de Tukey a 5% de significância. A adubação potássica e os clones de palma forrageira não exerceram influências significativas nas variáveis avaliadas durante a condução experimental. A adubação potássica não influencia as características estruturais, produtivas e teor potássico no crescimento inicial de clones de Nopalea cochenillifera. Palavras-chave: Nopalea cochenillifera; potássio; semiárido

Stress-induced c-Fos expression is differentially modulated by dexamethasone, diazepam and imipramine

Immobilization stress upregulates c-Fos expression in several CNS areas. Repeated stress or the use of drugs can modulate stress-induced c-Fos expression. Here, we investigated in 40 different areas of the rat brain the effects of dexamethasone (SDX, a synthetic glucocorticoid), diazepam (SBDZ, a benzodiazepine), and imipramine (IMI, an antidepressant) on the c-Fos expression induced by restraint stress. Wistar rats were divided into four groups and submitted to 20 days of daily injection of saline (three first groups) or imipramine, 15 mg/kg, i.p. On day 21, animals were submitted to injections of saline (somatosensory, SS), SDX (1 mg/kg, i.p.), SBDZ (5 mg/kg, i.p.), or IMI (15 mg/kg, i.p.) before being submitted to restraint. Immediately after stress, the animals were perfused and their brains processed with immunohistochemistry for c-Fos (Ab-5 Oncogene Science). Dexamethasone reduced stress- induced c-Fos expression in SS cortex, hippocampus, paraventricular nucleus of the hypothalamus (PVH), and locus coeruleus (LC), whereas diazepam reduced c-Fos staining in the SS cortex, hippocampus, bed nucleus of stria terminalis, septal area, and hypothalamus (preoptic area and supramammillary nucleus). Chronic administration of imipramine decreased staining in the hippocampus, PVH, and LC, while increasing it in the nucleus raphe pallidus. We conclude that dexamethasone, diazepam and imipramine differentially modulate stress-induced Fos expression. the present study provides an important comparative background that may help in the further understanding of the effects of these compounds and on the brain activation as well as on the behavioral, neuroendocrine, and autonomic responses to stress.UFRRJ, Dept Physiol Sci, BR-23890000 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Physiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, BrazilWeb of Scienc

Plant litter dynamics in the forest-stream interface: Precipitation is a major control across tropical biomes

Riparian plant litter is a major energy source for forested streams across the world and its decomposition has repercussions on nutrient cycling, food webs and ecosystem functioning. However, we know little about plant litter dynamics in tropical streams, even though the tropics occupy 40% of the Earth's land surface. Here we investigated spatial and temporal (along a year cycle) patterns of litter inputs and storage in multiple streams of three tropical biomes in Brazil (Atlantic forest, Amazon forest and Cerrado savanna), predicting major differences among biomes in relation to temperature and precipitation regimes. Precipitation explained most of litter inputs and storage, which were generally higher in more humid biomes (litterfall: 384, 422 and 308 g m-2 y-1, storage: 55, 113 and 38 g m-2, on average in Atlantic forest, Amazon and Cerrado, respectively). Temporal dynamics varied across biomes in relation to precipitation and temperature, with uniform litter inputs but seasonal storage in Atlantic forest streams, seasonal inputs in Amazon and Cerrado streams, and aseasonal storage in Amazon streams. Our findings suggest that litter dynamics vary greatly within the tropics, but point to the major role of precipitation, which contrasts with the main influence of temperature in temperate areas. © 2017 The Author(s)