Monocular tracking of the human arm in 3D: real-time implementation and experiments

We have developed a system capable of tracking a human arm in 3D and in real time. The system is based on a previously developed algorithm for 3D tracking which requires only a monocular view and no special markers on the body. In this paper we describe our real-time system and the insights gained from real-time experimentation

Two-parameter bifurcation analysis of the buck converter

This paper is concerned with the analysis of two-parameter bifurcation phenomena in the buck power converter. It is shown that the complex dynamics of the converter can be unfolded by considering higher codimension bifurcation points in two-parameter space. Specifically, standard smooth bifurcations are shown to merge with discontinuity-induced bifurcation (DIB) curves, giving rise to intricate bifurcation scenarios. The analytical results are compared with those obtained numerically, showing excellent agreement between the analytical predictions and the numerical observations. The existence of these two-parameter bifurcation phenomena involving DIBs and smooth bifurcations, predicted in [P. Kowalczyk et al., Internat. J. Bifur. Chaos Appl. Sci. Engrg., 16 (2006), pp. 601–629; A. Colombo and F. Dercole, SIAM J. Appl. Dyn. Syst., submitted], is confirmed in this important class of systems.Postprint (published version

Perspectivas críticas de los géneros de la literatura popular: policiaco y literatura especulativa en Hispanoamérica: Introducción

The present dossier proposes to establish a dialogue with recent critical and theoretical approaches through readings that address crime and speculative genre through a wide spectrum of perspectives, focusing on works by authors who claim their position in the traditions of detective fiction and speculative literature.El presente dossier propone establecer un diálogo con la recientes aproximaciones críticas y teóricas a través de lecturas que abordan el género policiaco y especulativo a través de un amplio espectro de perspectivas, centrándose en obras de autores/as que reivindican su posicionamiento en las tradiciones de las narrativas policíaca y de la literatura especulativa

On the use of information and infrastructure technologies for the smart city research in Europe: a survey

The Smart City paradigm has become one of the most important research topics around the globe. Particularly in Europe, it is considered as a solution for unstoppable increase of high density urban environments and the European Commission has included the Smart City research as one of the key objectives for the FP7 (Seventh Framework Program) and H2020 (Horizon 2020) research initiatives. As a result, a considerable amount of quality research, with particular emphasis on information and communication technologies, has been produced. In this paper, we review the current efforts dedicated in Europe to this research topic. Particular attention is paid in the review to the platforms and infrastructure technologies adopted to introduce the Internet of Things into the city, taking into account the constraints and harshness of urban environments. Furthermore, this paper also considers the efforts in the experimental perspective, which includes the review of existing Smart City testbeds, part of wider European initiatives such as FIRE (Future Internet Research and Experimentation) and FIWARE. Last but not least, the main efforts in providing interoperability between the different experimental facilities are also presented.This work was funded in part by the European Union’s Horizon 2020 Programme of the FESTIVAL project (Federated Interoperable Smart ICT Services Development and Testing Platforms) under grant agreement 643275, and from the Japanese National Institute of Information and Communications Technology

A reverse-engineering approach to dissect post-translational modulators of transcription factor's activity from transcriptional data.

BACKGROUND: Transcription factors (TFs) act downstream of the major signalling pathways functioning as master regulators of cell fate. Their activity is tightly regulated at the transcriptional, post-transcriptional and post-translational level. Proteins modifying TF activity are not easily identified by experimental high-throughput methods. RESULTS: We developed a computational strategy, called Differential Multi-Information (DMI), to infer post-translational modulators of a transcription factor from a compendium of gene expression profiles (GEPs). DMI is built on the hypothesis that the modulator of a TF (i.e. kinase/phosphatases), when expressed in the cell, will cause the TF target genes to be co-expressed. On the contrary, when the modulator is not expressed, the TF will be inactive resulting in a loss of co-regulation across its target genes. DMI detects the occurrence of changes in target gene co-regulation for each candidate modulator, using a measure called Multi-Information. We validated the DMI approach on a compendium of 5,372 GEPs showing its predictive ability in correctly identifying kinases regulating the activity of 14 different transcription factors. CONCLUSIONS: DMI can be used in combination with experimental approaches as high-throughput screening to efficiently improve both pathway and target discovery. An on-line web-tool enabling the user to use DMI to identify post-transcriptional modulators of a transcription factor of interest che be found at http://dmi.tigem.it

Human Transgene-Free Amniotic-Fluid-Derived Induced Pluripotent Stem Cells for Autologous Cell Therapy

The establishment of a reliable prenatal source of autologous, transgene-free progenitor cells has enormous potential in the development of regenerative-medicine-based therapies for infants born with devastating birth defects. Here, we show that a largely CD117-negative population of human amniotic fluid mesenchymal stromal cells (AF-MSCs) obtained from fetuses with or without prenatally diagnosed anomalies are readily abundant and have limited baseline differentiation potential when compared with bone-marrow-derived MSCs and other somatic cell types. Nonetheless, the AF-MSCs could be easily reprogrammed into induced pluripotent stem cells (iPSCs) using nonintegrating Sendai viral vectors encoding for OCT4, SOX2, KLF4, and cMYC. The iPSCs were virtually indistinguishable from human embryonic stem cells in multiple assays and could be used to generate a relatively homogeneous population of neural progenitors, expressing PAX6, SOX2, SOX3, Musashi-1, and PSA-NCAM, for potential use in neurologic diseases. Further, these neural progenitors showed engraftment potential in vivo and were capable of differentiating into mature neurons and astrocytes in vitro. This study demonstrates the usefulness of AF-MSCs as an excellent source for the generation of human transgene-free iPSCs ideally suited for autologous perinatal regenerative medicine applications.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140204/1/scd.2014.0110.pd

Lower lip squamous cell carcinoma in patients with photosensitive disorders : analysis of cases treated at the Brazilian National Cancer Institute (INCA) from 1999 to 2012

Lower lip squamous cell carcinoma (LLSCC) is a common malignancy of the head and neck, being mainly a consequence of a chronic exposure to ultraviolet (UV) light solar radiation. Here, we evaluated the clinicopathological profile of patients with photosensitive disorders (xeroderma pigmentosum, lupus erythematosus and albinism) that developed LLSCC. Data from patients who had a diagnosed LLSCC with a prior xeroderma pigmentosum, lupus erythematosus or albinism diagnosis that were treated at INCA from 1999 to 2012 were collected from patients? medical records (n=16). The control group was composed of 68 patients with LLSCC without a medical history of photosensitivity. The clinicopathological data of this study population were collected and the association between these variables was analyzed by Fisher?s exact test. Survival curves were constructed using the Kaplan?Meier method and compared by log-rank test. All statistical analyses were performed using SPSS statistics package. The mean age of patients in the photosensitive and non-photosensitive groups was 42 years and 67 years, respectively (p<0.0001). A previous history of malignant diseases was more common in the photosensitive group (p=0.001). In both groups, most tumors showed a pathological stage I/II disease. Overall and cancer-specific survival were not statistically different. However, disease-free interval showed a significant difference (p=0.01) between the photosensitive and non-photosensitive patients. Photosensitive patients presented LLSCC at earlier age but it usually was not the primary tumor in these patients. Furthermore, a more aggressive pathological behavior was not seen when compared with tumors from non-photosensitive patients. The disease-free interval was lower in photosensitive patients, as expected

Amblyomma sparsum Neumann 1899 on migratory birds from Africa : first records in Italy

DATA AVAILABILITY : Data will be made available on requestMigratory birds play an important role in transporting ixodid ticks and tick-borne pathogens between continents. During the Boreal spring, migratory birds reach Europe, mainly from sub-Saharan Africa or from northern African countries but not much is known about the diversity and ecology of the ticks they spread. From 2017 to 2022, in the framework of two consecutive projects focused on sampling migratory birds from Africa to Europe, a total of 27 immature Amblyomma ticks were collected from migratory birds, belonging to 8 species, captured on the Island of Ventotene, an important stop-over site in the Mediterranean Sea. In the absence of adult specimens, morphological identification was limited to assigning these ticks to the Amblyomma genus. In this study, sequencing and comparative analysis of three mitochondrial molecular markers (12S rDNA, 16S rDNA, COI) were performed to achieve taxonomic identification. Sequences obtained from Ventotene specimens matched at 100% identity with Amblyomma sparsum. In conclusion, this study documented that immature stages of this species belonging to the Amblyomma marmoreum complex reached the Pontine Islands for six consecutive years. The entry of alien tick species and their potentially transmitted pathogens deserves further study, also in light of the globally ongoing climate change.The Italian Ministry of Health and partially funded by EU funding within the NextGeneration EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases.https://www.elsevier.com/locate/ttbdishj2024Veterinary Tropical DiseasesSDG-03:Good heatlh and well-beingSDG-15:Life on lan

Genome-wide diversity, population structure and signatures of inbreeding in the African buffalo in Mozambique

DATA AVAILABILITY : All sequence data presented here (fastq format) have been deposited into the NCBI SRA database (accession number: PRJNA971211).The African buffalo, Syncerus caffer, is a key species in African ecosystems. Like other large herbivores, it plays a fundamental role in its habitat acting as an ecosystem engineer. Over the last few centuries, African buffalo populations have declined because of range contraction and demographic decline caused by direct or indirect human activities. In Mozambique, historically home to large buffalo herds, the combined effect of colonialism and subsequent civil wars has created a critical situation that urgently needs to be addressed. In this study, we focused on the analysis of genetic diversity of Syncerus caffer caffer populations from six areas of Mozambique. Using genome-wide SNPs obtained from ddRAD sequencing, we examined the population structure across the country, estimated gene flow between areas under conservation management, including national reserves, and assessed the inbreeding coefficients. Our results indicate that all studied populations of Syncerus caffer caffer are genetically depauperate, with a high level of inbreeding. Moreover, buffaloes in Mozambique present a significant population differentiation between southern and central areas. We found an unexpected genotype in the Gorongosa National Park, where buffaloes experienced a dramatic population size reduction, that shares a common ancestry with southern populations of Catuane and Namaacha. This could suggest the past occurrence of a connection between southern and central Mozambique and that the observed population structuring could reflect recent events of anthropogenic origin. All the populations analysed showed high levels of homozygosity, likely due to extensive inbreeding over the last few decades, which could have increased the frequency of recessive deleterious alleles. Improving the resilience of Syncerus caffer caffer in Mozambique is essential for preserving the ecosystem integrity. The most viable approach appears to be facilitating translocations and re-establishing connectivity between isolated herds. However, our results also highlight the importance of assessing intraspecific genetic diversity when considering interventions aimed at enhancing population viability such as selecting suitable source populations.This research was supported by the projects “Training in Biodiversity and Biotechnology for sustainable development” (AID 11096) and BioForMoz “Support for Environmental Research” (AID 12089) managed by the Department of Biomedical Sciences, University of Sassari, Italy, and funded by the Italian Agency for Development Cooperation, Italy. Open Access funding enabled and organized by Projekt DEAL.https://bmcecolevol.biomedcentral.comhj2024Veterinary Tropical DiseasesSDG-15:Life on lan