Relative expression of LMW-PTP and its <i>fast</i> and <i>slow</i> isoforms on the MDA-MB-435 cell line and knockdowns.

<p>Error bars represent standard deviation (n=4). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); CI, CII; CIII; CV – knockdown of total LMW-PTP; CIV – knockdown of LMW-PTP slow isoform.</p

Cell proliferation of different clones compared to KD NT (n=6).

<p>p > 0.05 for all comparisons. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.</p

Cell migration of LMW-PTP KDs and MDA-MB-435 compared to KD NT.

<p>A-E Representative images of cell migration assay. A: t=0, before migration; B-E: t=24h after migration. B: MDA-MB-435; C:KD NT; D:KD LMW-PTP; E: KD LMW-PTP <i>slow</i> isoform. Error bars represent standard deviation (n=3). *p<0.05 compared to KD NT and MDA-MB-435. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.</p

Dephosphorylated EphA2 in LMW-PTP KDs and MDA-MB-435.

<p>Error bars represent standard deviation (n=3). p > 0.05 for all comparisons. KD NT – scramble sequences siRNA (control); KD LMW-PTP – total LMW-PTP knockdown; KD LMW-PTP slow – LMW-PTP slow isoform knockdown.</p

RANKL/RANK/MMP-1 Molecular Triad Contributes to the Metastatic Phenotype of Breast and Prostate Cancer Cells <i>In Vitro</i>

<div><p>The osteolytic nature of bone metastasis results from a tumor-driven increased bone resorption. Bone remodeling is orchestrated by the molecular triad RANK-RANKL-OPG. This process is dysregulated in bone metastases, mostly via induction of RANKL by tumor-derived factors. These factors increase expression of RANKL, which induce osteoclast formation, function, and survival, thereby increasing bone resorption. RANK is unexpectedly expressed by cancer cells, and the activation of RANKL-RANK pathway correlates with an increased invasive phenotype. To investigate the interaction between RANK expression in human breast and prostate cancer cells and their pro-metastatic phenotype we analyzed the activation of RANKL-RANK pathway and its effects on cell migration, invasion, gene expression <i>in vitro</i>, and osteolysis-inducing ability <i>in vivo</i>. RANKL activates kinase signaling pathways, stimulates cell migration, increases cell invasion, and up-regulates MMP-1 expression. <i>In vivo</i>, MMP-1 knockdown resulted in smaller x-ray osteolytic lesions and osteoclastogenesis, and decreased tumor burden. Therefore, RANKL inhibition in bone metastatic disease may decrease the levels of the osteoclastogenesis inducer MMP-1, contributing to a better clinical outcome.</p></div

MOESM1 of Frequency and coinfection between genotypes of human papillomavirus in a population of asymptomatic women in northern Peru

Additional file 1: Table S1. Human papillomavirus types and oncogenic potential. Table S2. Demographics and characteristics among women with HPV

Dataset febriles Piura

<p><b>Detection and molecular characterization of Chikungunya and Zika in a dengue endemic region of the Peruvian coast</b><b> </b></p> <p><b> </b></p> <p><b>ABSTRACT</b></p> <p><b>Background:</b> Arboviral diseases has reemerged in the last years affecting primarily the coast of Peru. Piura is an endemic region of Dengue, where the recent appearance of Chikungunya (CHIKV) and Zika (ZIKV) has been renewed.</p> <p><b>Aim:</b> To assess the presence of DENV, CHIKV, and ZIKV in serum samples of patients with acute febrile illness in a locality ofPiura, Peru and describe associated clinical features.</p> <p><b>Methodology:</b> Descriptive cross-sectional study, sampling was done for convenience where it was obtained atotal of 496 serum samples from patients with acute febrile illness were collected from 18 primary care centers. All samples were analyzed via real-time RT-PCR to detect DENV, CHIKV, and ZIKV. In addition, positive samples for DENV were also processed for serotypes 1 to 4 classification via RT-PCR.</p> <p><b>Results:</b> DENV was the most common arbovirus detected in 170/496 (34.3%), followed by ZIKV in 39/496 (7.9%) and CHIKV in 23/496 (4.6%). Among the 170 samples positive for DENV, serotype 2 was the most predominant type present in 97/170 (57.1%) of samples, followed by the serotype 3 in 9/170 (5.3%). Headaches, muscle pain, and joint pain were the most common symptoms associated with fever in patients with DENV and ZIKV. No symptoms predominance was observed in patients with CHIKV.</p> <p><b>Conclusions:</b> </p> <p>DENV is considered the most frequent arbovirus in Peru and the number of cases has increased dramatically in the last 5 years. However, it is not the only arbovirus that circulates along the northern coast of Peru. It has also been determined the presence of ZIKV and CHIKV in our population, which may suggest the circulation of other arboviruses that have not been detected.</p> <p> </p> <p><b>Keywords</b><b>:</b> Peru, arbovirus, Dengue, Chikungunya, Zika, PCR</p

Dataset HPV 2018

<p><b>Frequency and coinfection between genotypes of Human papillomavirus in a population of asymptomatic women in northern Peru.</b></p><p><b><br></b></p><p></p><p><b>Objective:</b> Describe the prevalence of HPV genotypes via PCR and DNA sequencing in 397 women who attended to the gynecological outpatient clinic in the Hospital Regional Docente de Cajamarca from March to September 2017.</p> <p><b>Results:</b> A positive PCR result for HPV was observed in 121 cervical samples. A high-risk genotype was found in 63.6% (77/121) of patients, a probably oncogenic type in 23.1% (28/121) and a low-risk type in 7.4%. Among the high-risk genotypes, HPV-31 was the most common one present in 20% (21/77), followed by HPV-16 in 11.4% (12/77). Coinfections between two or more genotypes were observed in 12 cases.</p><b></b><p></p