Quantification of gliadin levels to the picogram level by flow cytometry

Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of gliadin. The peptide is common to the alpha-, beta-, gamma-, and omega-gliadins

Magnetic, electrical, and GPR waterborne surveys of moraine deposits beneath a lake: A case history from Turin, Italy

Bathymetry and bottom sediment types of inland water basins provide meaningful information to estimate water reserves and possible connections between surface and groundwater. Waterborne geophysical surveys can be used to obtain several independent physical parameters to study the sediments. We explored the possibilities of retrieving information on both shallow and deep geological structures beneath a morainic lake by means of waterborne nonseismic methods. In this respect, we discuss simultaneous magnetic, electrical, and groundpenetrating radar (GPR) waterborne surveys on the Candia morainic lake in northerly Turin (Italy).We used waterborne GPR to obtain information on the bottom sediment and the bathymetry needed to constrain the magnetic and electrical inversions. We obtained a map of the total magnetic field (TMF) over the lake from which we computed a 2D constrained compact magnetic inversion for selected profiles, along with a laterally constrained inversion for one electrical profile. The magnetic survey detected some deep anomalous bodies within the subbottom moraine. The electrical profiles gave information on the more superficial layer of bottom sediments. We identify where the coarse morainic material outcrops from the bottom finer sediments from a correspondence between high GPR reflectivity, resistivity, and magnetic anomalie

Generation of strong magnetic fields by r-modes in millisecond accreting neutron stars: induced deformations and gravitational wave emission

Differential rotation induced by the r-mode instability can generate very strong toroidal fields in the core of accreting, millisecond spinning neutron stars. We introduce explicitly the magnetic damping term in the evolution equations of the r-modes and solve them numerically in the Newtonian limit, to follow the development and growth of the internal magnetic field. We show that the strength of the latter can reach large values, $B \sim 10^{14}$ G, in the core of the fastest accreting neutron stars. This is strong enough to induce a significant quadrupole moment of the neutron star mass distribution, corresponding to an ellipticity |\epsilon_B}| \sim 10^{-8}. If the symmetry axis of the induced magnetic field is not aligned with the spin axis, the neutron star radiates gravitational waves. We suggest that this mechanism may explain the upper limit of the spin frequencies observed in accreting neutron stars in Low Mass X-Ray Binaries. We discuss the relevance of our results for the search of gravitational waves.Comment: 11 pages, 8 figure

Reaching the End of the White Dwarf Cooling Sequence in NGC 6791

We present new observations of the white dwarf sequence of the old open cluster NGC 6791. The brighter peak previously observed in the white dwarf luminosity function (WDLF) is now better delineated, and the second, fainter peak that we suggested earlier is now confirmed. A careful study suggests that we have reached the end of the white dwarf sequence. The WDs that create the two peaks in the WDLF show a significant turn to the blue in the color-magnitude diagram. The discrepancy between the age from the WDs and that from the main sequence turnoff remains, and we have an additional puzzle in the second peak in the WDLF. Canonical WD models seem to fail --at least at ~25%-level-- in reproducing the age of clusters of this metallicity. We discuss briefly possible ways of arriving at a theoretical understanding of the WDLF.Comment: 29 pages, 10 figures (4 in low resolution), 1 table. Accepted (2007 December 19) on Ap

Physical Adsorption at the Nanoscale: Towards Controllable Scaling of the Substrate-Adsorbate van der Waals Interaction

The Lifshitz-Zaremba-Kohn (LZK) theory is commonly considered as the correct large-distance limit for the van der Waals (vdW) interaction of adsorbates (atoms, molecules, or nanoparticles) with solid substrates. In the standard approximate form, implicitly based on "local" dielectric functions, the LZK approach predicts universal power laws for vdW interactions depending only on the dimensionality of the interacting objects. However, recent experimental findings are challenging the universality of this theoretical approach at finite distances of relevance for nanoscale assembly. Here, we present a combined analytical and numerical many-body study demonstrating that physical adsorption can be significantly enhanced at the nanoscale. Regardless of the band gap or the nature of the adsorbate specie, we find deviations from conventional LZK power laws that extend to separation distances of up to 10--20 nanometers. Comparison with recent experimental observation of ultra long-ranged vdW interactions in the delamination of graphene from a silicon substrate reveals qualitative agreement with the present theory. The sensitivity of vdW interactions to the substrate response and to the adsorbate characteristic excitation frequency also suggests that adsorption strength can be effectively tuned in experiments, paving the way to an improved control of physical adsorption at the nanoscale

Why haven't loose globular clusters collapsed yet?

We report on the discovery of a surprising observed correlation between the slope of the low-mass stellar global mass function (GMF) of globular clusters (GCs) and their central concentration parameter c=log(r_t/r_c), i.e. the logarithmic ratio of tidal and core radii. This result is based on the analysis of a sample of twenty Galactic GCs with solid GMF measurements from deep HST or VLT data. All the high-concentration clusters in the sample have a steep GMF, most likely reflecting their initial mass function. Conversely, low-concentration clusters tend to have a flatter GMF implying that they have lost many stars via evaporation or tidal stripping. No GCs are found with a flat GMF and high central concentration. This finding appears counter-intuitive, since the same two-body relaxation mechanism that causes stars to evaporate and the cluster to eventually dissolve should also lead to higher central density and possibly core-collapse. Therefore, more concentrated clusters should have lost proportionately more stars and have a shallower GMF than low concentration clusters, contrary to what is observed. It is possible that severely depleted GCs have also undergone core collapse and have already recovered a normal radial density profile. It is, however, more likely that GCs with a flat GMF have a much denser and smaller core than suggested by their surface brightness profile and may well be undergoing collapse at present. In either case, we may have so far seriously underestimated the number of post core-collapse clusters and many may be lurking in the Milky Way.Comment: Four pages, one figure, accepted for publication in ApJ Letter

Sulodexide counteracts endothelial dysfunction induced by metabolic or non-metabolic stresses through activation of the autophagic program

OBJECTIVE: Endothelial dysfunction (ED) predisposes to venous thrombosis (VT) and post-thrombotic syndrome (PTS), a long-term VT-related complication. Sulodexide (SDX) is a highly purified glycosaminoglycan with antithrombotic, pro-fibrinolytic and anti-inflammatory activity used in the treatment of chronic venous disease (CVD), including patients with PTS. SDX has recently obtained clinical evidence in the “extension therapy” after initial-standard anticoagulant treatment for the secondary prevention of recurrent deep vein thrombosis (DVT). Herein, we investigated how SDX counteracts ED. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVEC) were used. Metabolic and non metabolic-induced ED was induced by treating with methylglyoxal (MGO) or irradiation (IR), respectively. Bafilomycin A1 was used to inhibit autophagy. The production of reactive oxygen species (ROS), tetrazolium bromide (MTT) assay for cell viability, terminal de-oxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay for cell apoptosis, Real-time PCR and Western blot analysis for gene and protein expression were used. RESULTS: SDX protected HUVEC from MGO- or IR-induced apoptosis by counteracting the activation of the intrinsic and extrinsic caspase cascades. The cytoprotective effects of SDX resulted from a reduction in a) ROS production, b) neo-synthesis and release of pro-inflammatory cytokines (TNFα, IL1, IL6, IL8), c) DNA damage induced by MGO or IR. These effects were reduced when autophagy was inhibited. CONCLUSIONS: Data herein collected indicate the ability of SDX to counteract ED induced by metabolic or non-metabolic stresses by involving the intracellular autophagy pathway. Our experience significantly increases the knowledge of the mechanisms of action of SDX against ED and supports the use of SDX in the treatment of CVD, PTS and in the secondary prevention of recurrent DVT

First Record of Emys orbicularis (Boulenger, 1882), (Reptilia, Testudinati) in the “Castel di Guido” Natural Park (Northern Latium, Italy): a Case of Interest for Species Conservation

The finding of five specimens of Emys orbicularis (Linnaeus, 1758), (Reptilia, Testudinati) in the “Castel di Guido” Natural Park (Northern Latium, Italy), is reported. This is the first record for the species in the area, regularly monitored since 2006. It is assumed that the species has recently colonized the site through highly populated and degraded territory; the data is of conservation interest, proving the capacity of the species to cross polluted environments

Thick tori around AGN: the case for extended tori and consequences for their X-ray and IR emission

Two families of models of dusty tori in AGNs (moderately thick and extended versus very thick and compact) are tested against available observations. The confrontation suggests that the former class better explains the IR broad-band spectra of both broad and narrow line AGNs, the anisotropy of the emission deduced by comparing IR properties of Seyfert 1 and 2 nuclei, the results of IR spectroscopy and those of high spatial resolution observations. There is however clear evidence for a broad distribution of optical depths. We also examine the relationship between IR and X-ray emission. The data support a view in which the matter responsible for the X-ray absorption is mostly dust free, lying inside the dust sublimation radius. The consequences of these results for the hard X-ray background as well as IR counts and background are discussed.Comment: 33 pages, 9 Postscript figures, to appear in ApJ, September 199