295 research outputs found
Proteome profiling by label‐free mass spectrometry reveals differentiated response of Campylobacter jejuni 81–176 to sublethal concentrations of bile acids.
Purpose Bile acids are crucial components of the intestinal antimicrobial defense and represent a significant stress factor for enteric pathogens. Adaptation processes of Campylobacter jejuni to this hostile environment are analyzed in this study by a proteomic approach. Experimental design Proteome profiling by label-free mass spectrometry (SWATH-MS) has been used to characterize the adaptation of C. jejuni to sublethal concentrations of seven bile acids. Results The bile acids with the lowest inhibitory concentration (IC50), deoxycholic and chenodeoxycholic acid, induce the most significant proteome changes. Overall a downregulation of all basic biosynthetic pathways and a general decrease in the transcription machinery are found. Concurrently, an induction of factors involved in detoxification of reactive oxygen species, protein folding, and bile acid exporting efflux pumps is detected. Exposure to deoxycholic and chenodeoxycholic acid results in an increased expression of components of the more energy-efficient aerobic respiration pathway, while the anaerobic branches of the electron transport chain are down-expressed. Conclusions and clinical relevance The results show that C. jejuni has a differentiated system of adaptation to bile acid stresses. The findings enhance the understanding of the pathogenesis of campylobacteriosis, especially for survival of C. jejuni in the human intestine, and may provide clues to future medical treatment
Characterization of Human Endogenous Retrovirus Type K Virus-like Particles Generated from Recombinant Baculoviruses
AbstractThe family of human endogenous retrovirus type K (HERV-K) comprises members with long open reading frames (ORF) for retroviral proteins. The existence of a biologically active provirus with replicative capacities has not yet been demonstrated. To confirm the assumption that HERV-K codes for the previously observed retrovirus-like particles (human teratocarcinoma-derived virus, HTDV) in human teratocarcinoma cells, we have constructed recombinant full-length HERV-K cDNA-based baculoviruses withgag, pro, pol,andenvORFs. Two viral constructs were used for infections of insect cells, one bearing 67 bp of the 5′ untranslated region upstream of the 5′ splice donor (SD) site and of the retroviral genes, the second omitting the SD sequence. For both recombinant viruses, indirect immunofluorescence and laser scan analyses revealed expression of HERV-K Gag protein. Electron microscopy studies demonstrated efficient production of virus-like particles (VLPs) at the cytoplasmic cell membranes. These VLPs are morphologically identical with the HTDV phenotype. In immunoelectron microscopy of ultrathin frozen sections, anti-HERV-K Gag antibodies specifically reacted with HERV-K VLPs. In Western blots, in addition to the 76-kDa precursor protein, the putative major core protein with an apparent molecular mass of 32 kDa exhibited predominant immunoreactivity with anti-Gag antiserum. In contrast, neither HERV-K Env nor cORF proteins could be detected due to inefficient mRNA splicing. Purified particles from insect cell culture supernatants tested in an ultrasensitive reverse transcriptase assay revealed weak polymerase activity. The data demonstrate that HERV-K codes for retroviral particles of the HTDV phenotype
Measurement of the Beam Asymmetry in the Forward Direction for pi0 Photoproduction
Photoproduction of neutral pions has been studied with the CBELSA/TAPS
detector for photon energies between 0.92 and 1.68~GeV at the electron
accelerator ELSA. The beam asymmetry~ has been extracted for of the ~meson and for . The new beam asymmetry data improve the world database for
photon energies above 1.5~GeV and, by covering the very forward region, extend
previously published data for the same reaction by our collaboration. The
angular dependence of shows overall good agreement with the SAID
parameterization.Comment: 11 pages, 10 figures, published in Phys. Rev. C, included LEPS data
and MAID 2007 predictions for comparison with our dat
Neutron skin of Pb from Coherent Pion Photoproduction
Information on the size and shape of the neutron skin on Pb has been
extracted from coherent pion photoproduction cross sections measured using the
Crystal Ball together with the Glasgow tagger at the MAMI electron beam
facility. On exploitation of an interpolated fit of a theoretical model to the
measured cross sections the half-height radius and diffuseness of the neutron
distribution are found to be 6.70 fm and 0.55 fm respectively, corresponding to a neutron
skin thickness =0.15 fm.
The results give the first successful extraction of a neutron skin with an
electromagnetic probe and indicate the skin of Pb has a halo character.
The measurement provides valuable new constraints on both the structure of
nuclei and the equation of state for neutron-rich matter.Comment: 4 figures 5 pages. Version submitted to journal. Includes additional
studies of systematic effects in the extracted diffuseness, which led to a
small increase in the quoted systematic error. These additional studies are
discussed in the revised manuscript. Also includes minor editorial
improvements to the tex
Photoproduction of Neutral Pions off Protons
Photoproduction of neutral pions has been studied with the CBELSA/TAPS
detector in the reaction for photon energies between 0.85
and 2.50 GeV. The mesons are observed in their dominant neutral decay
mode: . For the first time, the differential cross
sections cover the very forward region, . A
partial-wave analysis of these data within the Bonn-Gatchina framework observes
the high-mass resonances (2190), (2080), and (2070).Comment: 12 pages, 9 figures, minor modifications to PWA sectio
Modification of the -Meson Lifetime in Nuclear Matter
The photo production of mesons on the nuclei C, Ca, Nb and Pb has
been measured using the Crystal Barrel/TAPS detector at the ELSA tagged photon
facility in Bonn. The dependence of the meson cross section on the
nuclear mass number has been compared with three different types of models, a
Glauber analysis, a BUU analysis of the Giessen theory group and a calculation
by the Valencia theory group. In all three cases, the inelastic width
is found to be at normal nuclear matter density for an
average 3-momentum of 1.1 GeV/c. In the restframe of the meson, this
inelastic width corresponds to a reduction of the lifetime by
a factor . For the first time, the momentum dependent N
cross section has been extracted from the experiment and is in the range of 70
mb.Comment: 5 pages, 4 figure
Determination of the Dalitz plot parameter alpha for the decay eta->3pi^0 with the Crystal Ball at MAMI-B
A precise measurement of the Dalitz plot parameter, alpha, for the eta->3pi^0
decay is presented. The experiment was performed with the Crystal Ball and TAPS
large acceptance photon detectors at the tagged photon beam facility of the
MAMI-B electron accelerator in Mainz. High statistics of 1.8*10^6 eta->3pi^0
events were obtained, giving the result alpha = -0.032 +/- 0.002(stat) +/-
0.002(syst).Comment: 9 pages, 6 figures, published in the online-first section of EPJ A,
included changes referees asked for, added DO
Photoproduction of meson pairs: First measurement of the polarization observable I^s
The polarization observable I^s, a feature exclusive to the acoplanar
kinematics of multi-meson final states produced via linearly polarized photons,
has been measured for the first time. Results for the reaction g p -> p pi0 eta
are presented for incoming photon energies between 970 MeV and 1650 MeV along
with the beam asymmetry I^c. The comparably large asymmetries demonstrate a
high sensitivity of I^s to the dynamics of the reaction. Fits using
Bonn-Gatchina partial wave analysis demonstrate that the new polarization
observables carry significant information on the contributing partial waves.Comment: 11 pages, 6 figures, v2 to appear in Phys. Lett.
Photoproduction of {\omega} Mesons off the Proton
The differential cross sections and unpolarized spin-density matrix elements
for the reaction were measured using the CBELSA/TAPS
experiment for initial photon energies ranging from the reaction threshold to
2.5 GeV. These observables were measured from the radiative decay of the
meson, . The cross sections cover the full
angular range and show the full extent of the -channel forward rise. The
overall shape of the angular distributions in the differential cross sections
and unpolarized spin-density matrix elements are in fair agreement with
previous data. In addition, for the first time, a beam of linearly-polarized
tagged photons in the energy range from 1150 MeV to 1650 MeV was used to
extract polarized spin-density matrix elements.
These data were included in the Bonn-Gatchina partial wave analysis (PWA).
The dominant contribution to photoproduction near threshold was found
to be the partial wave, which is primarily due to the sub-threshold
resonance. At higher energies, pomeron-exchange was found to
dominate whereas -exchange remained small. These -channel contributions
as well as further contributions from nucleon resonances were necessary to
describe the entire dataset: the , , and partial waves
were also found to contribute significantly.Comment: 8 pages, 5 figures, accepted for publication in Physics Letters
High statistics study of the reaction
The photoproduction of 2 mesons off protons was studied with the
Crystal Barrel/TAPS experiment at the electron accelerator ELSA in Bonn. The
energy of photons produced in a radiator was tagged in the energy range from
600\,MeV to 2.5\,GeV. Differential and total cross sections and
Dalitz plots are presented. Part of the data was taken with a diamond radiator
producing linearly polarized photons, and beam asymmetries were derived.
Properties of nucleon and resonances contributing to the
final state were determined within the BnGa partial wave analysis. The data
presented here allow us to determine branching ratios of nucleon and
resonances for their decays into via several intermediate states.
Most prominent are decays proceeding via , ,
, , but also , , and
contribute to the reaction.Comment: 28 pages, 17 figures, 7 table
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