Study of associations of polymorphic variants of lipid metabolism genes and angiogenesis with first ischemic stroke

Introduction. Ischemic stroke (IS) is a complex multifactorial disorder with heterogeneous congenital component. Polymorphic variants of genes of apolipoprotein В (APOB), apolipoprotein А-V (APOА-V), apolipoprotein С-IV (APOC-IV), apolipoprotein H (APOH), apolipoprotein D (APOD), angiopoietin-related protein 4 (ANGPT4) that regulate lipid metabolism and vascular regeneration, may be regarded as perspective ones. Although until recently they were not studied in complex, in a homogenous group from the perspective of possible association with first IS development. Aim. To study association between polymorphic variants of the following genes: АРОH, APOD, APOE, APOA5, APOC4, ANGPT4 and first IS development. Materials and methods. Frequencies of alleles and genotypes of single nucleotide polymorphic gene variants in 200 patients after first IS and in 115 people without stroke matched by sex, age and place of residence were studied. Blood was taken from all study participants in order to perform genotyping assay using protocol by J.Hixson and D.Vernier. Genomic deoxyribonucleic acid extraction from blood was performed using magnetic beads technology on an automated system for nucleic acid extraction Chemagen Prepito (ABBIS, Germany). For single nucleotide polymorphisms typing alleles identification analysis was performed using polymerase chain reaction with prepared TaqMan probes with Assey ID identification number (Applied Biosystems, USA). Amplification of polymorphic regions of the genes was performed with the use of 7500 Real-Time PCR System (Applied Biosystems, USA). Standard amplification conditions were used that were compatible with those defined by chemical agents supplier for each Assey ID. Genotypes nomenclature is given in accordance with international database db SNP (https://www.ncbi.nlm.nih.gov/SNP/). Results. Significant differences between groups in distribution of minor alleles and genotypes were acquired for АРОD (rs7659) and APOA5 (rs619054) polymorphisms. Conclusion. Significant association of single nucleotide АРОD (rs7659) and APOA-V (rs619054) genes polymorphisms with first IS development was found in study groups

Risk for Major Bleeding in Patients Receiving Ticagrelor Compared With Aspirin After Transient Ischemic Attack or Acute Ischemic Stroke in the SOCRATES Study (Acute Stroke or Transient Ischemic Attack Treated With Aspirin or Ticagrelor and Patient Outcomes)

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