Analysis of the Movement of Chlamydomonas Flagella: The Function of the Radial-spoke System Is Revealed by Comparison of Wild-type and Mutant Flagella

The mutation uni-1 gives rise to uniflagellate Chlamydomonas cells which rotate around a fixed point in the microscope field, so that the flagellar bending pattern can be photographed easily . This has allowed us to make a detailed analysis of the wild-type flagellar bending pattern and the bending patterns of flagella on several mutant strains. Cells containing uni-1, and recombinants of uni-1 with the suppressor mutations, sup(_pf)-1 and sup(_pf)-3, show the typical asymmetric bending pattern associated with forward swimming in Chlamydomonas, although sup(_pf)-1 flagella have about one-half the normal beat frequency, apparently as the result of defective function of the outer dynein arms. The pf-17 mutation has been shown to produce nonmotile flagella in which radial spoke heads and five characteristic axonemal polypeptides are missing. Recombinants containing pf-17 and either sup(_pf)-1 or sup(_pf)-3 have motile flagella, but still lack radial-spoke heads and the associated polypeptides . The flagellar bending pattern of these recombinants lacking radial-spoke heads is a nearly symmetric, large amplitude pattern which is quite unlike the wild-type pattern . However, the presence of an intact radial-spoke system is not required to convert active sliding into bending and is not required for bend initiation and bend propagation, since all of these processes are active in the sup(_pf) pf-17 recombinants. The function of the radial-spoke system appears to be to convert the symmetric bending pattern displayed by these recombinants into the asymmetric bending pattern required for efficient swimming, by inhibiting the development of reverse bends during the recovery phase of the bending cycle

Simple Cardiac Screening of NCAA and USAC Collegiate Athletes Using Smartphone Electrocardiogram

Please refer to the pdf version of the abstract located adjacent to the title

Statistics of quantum transmission in one dimension with broad disorder

We study the statistics of quantum transmission through a one-dimensional disordered system modelled by a sequence of independent scattering units. Each unit is characterized by its length and by its action, which is proportional to the logarithm of the transmission probability through this unit. Unit actions and lengths are independent random variables, with a common distribution that is either narrow or broad. This investigation is motivated by results on disordered systems with non-stationary random potentials whose fluctuations grow with distance. In the statistical ensemble at fixed total sample length four phases can be distinguished, according to the values of the indices characterizing the distribution of the unit actions and lengths. The sample action, which is proportional to the logarithm of the conductance across the sample, is found to obey a fluctuating scaling law, and therefore to be non-self-averaging, in three of the four phases. According to the values of the two above mentioned indices, the sample action may typically grow less rapidly than linearly with the sample length (underlocalization), more rapidly than linearly (superlocalization), or linearly but with non-trivial sample-to-sample fluctuations (fluctuating localization).Comment: 26 pages, 4 figures, 1 tabl

Spectral properties of zero temperature dynamics in a model of a compacting granular column

The compacting of a column of grains has been studied using a one-dimensional Ising model with long range directed interactions in which down and up spins represent orientations of the grain having or not having an associated void. When the column is not shaken (zero 'temperature') the motion becomes highly constrained and under most circumstances we find that the generator of the stochastic dynamics assumes an unusual form: many eigenvalues become degenerate, but the associated multi-dimensional invariant spaces have but a single eigenvector. There is no spectral expansion and a Jordan form must be used. Many properties of the dynamics are established here analytically; some are not. General issues associated with the Jordan form are also taken up.Comment: 34 pages, 4 figures, 3 table

Recalibration Methodology to Compensate for Changing Fluid Properties in an Individual Nozzle Direct Injection Systems

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were to (1) develop a method for continuous recalibration of the chemical concentrate injectors to ensure accurate metering of chemicals of varying viscosities and (2) evaluate the recalibration method for estimating individual injector flow rates from a system of multiple injectors to assess potential errors. Test results indicated that the recalibration method was able to compensate for changes in fluid kinematic viscosity (e.g., from temperature changes and/or product variation). Errors were less than 3.4% for the minimum injector duty cycle (DCi) (at 10%) and dropped 0.2% for the maximum DCi (at 90%) for temperature changes of up to 20°C. While larger temperature changes may be expected, these test results showed that the proposed method could be successfully implemented to meet desired injection rates. Because multiple injectors would be used in commercial deployment of this technology, a method was developed to calculate the desired injector flow rate using initial injector calibration factors. Using this multi-injector recalibration method, errors ranged from 0.23% to 0.66% between predicted and actual flow rates for all three injectors

Recalibration Methodology to Compensate for Changing Fluid Properties in an Individual Nozzle Direct Injection Systems

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were to (1) develop a method for continuous recalibration of the chemical concentrate injectors to ensure accurate metering of chemicals of varying viscosities and (2) evaluate the recalibration method for estimating individual injector flow rates from a system of multiple injectors to assess potential errors. Test results indicated that the recalibration method was able to compensate for changes in fluid kinematic viscosity (e.g., from temperature changes and/or product variation). Errors were less than 3.4% for the minimum injector duty cycle (DCi) (at 10%) and dropped 0.2% for the maximum DCi (at 90%) for temperature changes of up to 20°C. While larger temperature changes may be expected, these test results showed that the proposed method could be successfully implemented to meet desired injection rates. Because multiple injectors would be used in commercial deployment of this technology, a method was developed to calculate the desired injector flow rate using initial injector calibration factors. Using this multi-injector recalibration method, errors ranged from 0.23% to 0.66% between predicted and actual flow rates for all three injectors

Recalibration Methodology to Compensate for Changing Fluid Properties in an Individual Nozzle Direct Injection Systems

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were to (1) develop a method for continuous recalibration of the chemical concentrate injectors to ensure accurate metering of chemicals of varying viscosities and (2) evaluate the recalibration method for estimating individual injector flow rates from a system of multiple injectors to assess potential errors. Test results indicated that the recalibration method was able to compensate for changes in fluid kinematic viscosity (e.g., from temperature changes and/or product variation). Errors were less than 3.4% for the minimum injector duty cycle (DCi) (at 10%) and dropped 0.2% for the maximum DCi (at 90%) for temperature changes of up to 20°C. While larger temperature changes may be expected, these test results showed that the proposed method could be successfully implemented to meet desired injection rates. Because multiple injectors would be used in commercial deployment of this technology, a method was developed to calculate the desired injector flow rate using initial injector calibration factors. Using this multi-injector recalibration method, errors ranged from 0.23% to 0.66% between predicted and actual flow rates for all three injectors

Statistics of low energy excitations for the directed polymer in a 1+d1+d random medium (d=1,2,3d=1,2,3)

We consider a directed polymer of length $L$ in a random medium of space dimension $d=1,2,3$. The statistics of low energy excitations as a function of their size $l$ is numerically evaluated. These excitations can be divided into bulk and boundary excitations, with respective densities $\rho^{bulk}_L(E=0,l)$ and $\rho^{boundary}_L(E=0,l)$. We find that both densities follow the scaling behavior $\rho^{bulk,boundary}_L(E=0,l) = L^{-1-\theta_d} R^{bulk,boundary}(x=l/L)$, where $\theta_d$ is the exponent governing the energy fluctuations at zero temperature (with the well-known exact value $\theta_1=1/3$ in one dimension). In the limit $x=l/L \to 0$, both scaling functions $R^{bulk}(x)$ and $R^{boundary}(x)$ behave as $R^{bulk,boundary}(x) \sim x^{-1-\theta_d}$, leading to the droplet power law $\rho^{bulk,boundary}_L(E=0,l)\sim l^{-1-\theta_d}$ in the regime $1 \ll l \ll L$. Beyond their common singularity near $x \to 0$, the two scaling functions $R^{bulk,boundary}(x)$ are very different : whereas $R^{bulk}(x)$ decays monotonically for $0<x<1$, the function $R^{boundary}(x)$ first decays for $0<x<x_{min}$, then grows for $x_{min}<x<1$, and finally presents a power law singularity $R^{boundary}(x)\sim (1-x)^{-\sigma_d}$ near $x \to 1$. The density of excitations of length $l=L$ accordingly decays as $\rho^{boundary}_L(E=0,l=L)\sim L^{- \lambda_d}$ where $\lambda_d=1+\theta_d-\sigma_d$. We obtain $\lambda_1 \simeq 0.67$, $\lambda_2 \simeq 0.53$ and $\lambda_3 \simeq 0.39$, suggesting the possible relation $\lambda_d= 2 \theta_d$.Comment: 15 pages, 25 figure