Mass spectrometric imaging of cysteine rich proteins in human skin

L

Histologically differing regions identified in hematoxylin-eosin-stained skin melanoma of a 10-week-old MeLiM.

<p>(A) Growing melanoma tissue (GMT), (B) melanoma tissue with early spontaneous regression (ESR), and (C) melanoma tissue with late spontaneous regression (LSR) (scale bar = 20 μm).</p

Histologically differing regions identified in hematoxylin-eosin-stained skin melanoma of a 10-week-old MeLiM.

<p>(A) Growing melanoma tissue (GMT), (B) melanoma tissue with early spontaneous regression (ESR), and (C) melanoma tissue with late spontaneous regression (LSR) (scale bar = 20 μm).</p

Histology, scan and MSI image of the mass ion peak at <i>m/z</i> 6011 with specified regions of differentiated tissue sections (differences between MeLiM melanoma and healthy tissue).

<p>A) Three histologically differing regions identified in hematoxylin-eosin-stained skin from porcine melanoma: red–normally growing melanoma tissue (GMT), violet–early spontaneous regression (ESR), and orange–late spontaneous regression (LSR). B) Six ROIs determined in healthy skin tissue: red–epidermis, light blue–dermis, violet–hair follicle, dark blue–sweat gland, orange–subcutaneous adipose tissue, and green–subcutaneous muscle.</p

Tentative identification of proteins with significant variation between MeLiM melanoma and healthy skin tissue.

<p>Tentative identification of proteins with significant variation between MeLiM melanoma and healthy skin tissue.</p

Schematic of MALDI MSI of MeLiM healthy skin/melanoma tissue.

<p>Schematic of MALDI MSI of MeLiM healthy skin/melanoma tissue.</p