Phase behavior of wormlike rods

By employing Molecular Dynamics computer simulations, the phase behavior of systems of rodlike particles with varying degree of internal flexibility has been traced from the perfectly rigid rod limit till very flexible particles, and from the high density region till the isotropic phase. From the perfectly rigid rod limit and enhancing the internal flexibility, the range of the smectic A phase is squeezed out by the concomitant action of the scarcely affected crystalline phase at higher density and the nematic phase at lower density, until it disappears. These results confirm the supposition, drawn from previous theoretical, simulational and experimental studies, that the smectic A phase is destabilized by introducing and enhancing the degree of particle internal flexibility. However, no significant changes in the order of nematic--to--smectic A phase transition, which appears always first order, nor in the value of the layer spacing, are observed upon varying the degree of particle internal flexibility. Moreover, no evidence of a columnar phase, which was tought of as a possible superseder of the smectic A phase in flexible rods, has been obtained.Comment: 10 pages, 2 figures, version accepted in Physical Review

Anxiety associated with palatable food withdrawal is reversed by the selective FAAH inhibitor PF-3845: A regional analysis of the contribution of endocannabinoid signaling machinery

Objective: Consumption of energy-dense palatable "comfort" food can alleviate stress and negative emotions, while abrupt withdrawal from a palatable diet can worsen these symptoms, causing difficulties with adherence to weight-loss diets. Currently, no pharmacological treatment is effective for obesity-related anxiety, so we investigated the endocannabinoid system (ECS), and specifically the fatty acid amide hydrolase (FAAH), as an interesting emerging target in this context because of its key role in the regulation of both energy homeostasis and emotional behavior. Methods: Rats were subjected to exposure and subsequent abstinence from a palatable cafeteria diet. During abstinence period, rats were treated with the selective FAAH inhibitor PF-3845 (10 mg/kg; intraperitoneal administration every other day). Results: Abstinent rats displayed an anxiogenic-like behavior and changes in the proteins of ECS signaling machinery in brain areas involved both in anxiety and food intake regulation. In particular, withdrawal caused a reduction of the expression of cannabinoid receptors in the nucleus accumbens and of enzymes diacylglycerol lipase alpha and monoacylglycerol lipase (MAGL) in the amygdala. Pharmacological inhibition of FAAH exerted an anxiolytic-like effect in abstinent animals and increased both MAGL expression in amygdala and CB2 expression in prefrontal cortex. Discussion: Overall, our results suggest that emotional disturbances associated with dieting are coupled with region-specific alterations in the cerebral expression of the ECS and that the enhancement of the endocannabinoid signaling by FAAH inhibition might represent a novel pharmacological strategy for the treatment of anxiety related to abstinence from palatable food.Funding for open access charge: Universidad de Málaga / CBUA European Regional DevelopmentFunds-European Union, Grant/Award Number:PI19/01577; Instituto de Salud Carlos III,Grant/Award Number: RETICS; Ministerio deCiencia e Innovaci on, Grant/Award Number:ERDF-EU RD16/0017/0001; Ministerodell'Università e della Ricerca, Grant/AwardNumber: 2012JTX3KL; PNRR-RomeTechnopole-FP

Cannabidiol Reduces Intestinal Inflammation through the Control of Neuroimmune Axis

Enteric glial cells (EGC) actively mediate acute and chronic inflammation in the gut; EGC proliferate and release neurotrophins, growth factors, and pro-inflammatory cytokines which, in turn, may amplify the immune response, representing a very important link between the nervous and immune systems in the intestine. Cannabidiol (CBD) is an interesting compound because of its ability to control reactive gliosis in the CNS, without any unwanted psychotropic effects. Therefore the rationale of our study was to investigate the effect of CBD on intestinal biopsies from patients with ulcerative colitis (UC) and from intestinal segments of mice with LPS-induced intestinal inflammation. CBD markedly counteracted reactive enteric gliosis in LPS-mice trough the massive reduction of astroglial signalling neurotrophin S100B. Histological, biochemical and immunohistochemical data demonstrated that S100B decrease was associated with a considerable decrease in mast cell and macrophages in the intestine of LPS-treated mice after CBD treatment. Moreover the treatment of LPS-mice with CBD reduced TNF-α expression and the presence of cleaved caspase-3. Similar results were obtained in ex vivo cultured human derived colonic biopsies. In biopsies of UC patients, both during active inflammation and in remission stimulated with LPS+INF-γ, an increased glial cell activation and intestinal damage were evidenced. CBD reduced the expression of S100B and iNOS proteins in the human biopsies confirming its well documented effect in septic mice. The activity of CBD is, at least partly, mediated via the selective PPAR-gamma receptor pathway. CBD targets enteric reactive gliosis, counteracts the inflammatory environment induced by LPS in mice and in human colonic cultures derived from UC patients. These actions lead to a reduction of intestinal damage mediated by PPARgamma receptor pathway. Our results therefore indicate that CBD indeed unravels a new therapeutic strategy to treat inflammatory bowel diseases

Shaking table tests and numerical analyses on a scaled dry-joint arch undergoing windowed sine pulses

The damages occurred during recent seismic events have emphasised the vulnerability of vaulted masonry structures, one of the most representative elements of worldwide cultural heritage. Although a certain consensus has been reached regarding the static behaviour of masonry arches, still more efforts are requested to investigate their dynamic behaviour. In this regard, the present paper aims to investigate the performance of a scaled dry-joint arch undergoing windowed sine pulses. A feature tracking based measuring technique was employed to evaluate the displacement of selected points, shading light on the failure mechanisms and gathering data for the calibration of the numerical model. This was built according to a micro-modelling approach of the finite element method, with voussoirs assumed very stiff and friction interface elements. Comparisons with existing literature are also stressed, together with comments about scale effects.This work was partly financed by FEDER funds through the Competitivity Factors Operational Programme-COMPETE and by national funds through FCT-Foundation for Science and Technology within the scope of the Project POCI-01-0145-FEDER-007633.info:eu-repo/semantics/publishedVersio

Structure and dynamics of liquid crystals from computer simulation on simple and atomic-detail models

In this thesis we study structure and dynamics about atomistic detail liquid crystal belonging to nCB series and a simple model liquid crystal. We payed particular attention in veryfing that little differences exist at the short range between nematic and isotropic phases; moreover we payed particular attention describing structural features of the so evanescen smectic B phase. We focused our attention in microscopic mechanism of diffusion in smectic A, nematic and isotropic phases. A subdiffisuion behavior of translational and rotational degrees of freedom has been found for the nCB series. From the point of view of the theory, we adopted DFT theory with an extension of the Parsons Lee approach to model the phase diagram, while we exploited MAT (modified affine theory) to fit translational diffusion coefficients

A portable NMR sensor for moisture monitoring of wooden works of art, particularly of painting on wood

It is proposed the use of a mobile device based on a NMR single-sided sensor for in situ non-invasive determination of the moisture content (MC) of wood items, especially items of Cultural Heritage interest. The MC is obtained through the moisture volume fraction, which is an appropriate quantity for the sensor and corresponds to the fraction of its measurements sensitive volume occupied by water. The device has been used here to track changes in MC of wood specimens caused by changes over time of the environmental relative humidity. The kinetics of water adsorption has been related to results obtained with the gravimetric method. Measurements on an old painting, the Pietà (1516–1517), oil on a poplar wood panel by Sebastiano del Piombo (1485 Venice, 1547 Rome), Civic Museum, Viterbo, Italy, have shown, conclusively, the good sensitivity of the sensor and its capability to behave as a non-invasive and in situ utilizable device. Results of in situ painting measurements show that the NMR sensor can track moisture fluctuations that are outside the sensitivity range and precision of electro-hygrometric approach

Lymph node micrometastasis and survival of patients with Stage I (Dukes' A) colorectal carcinoma.

Objective. Although patients with Stage I colorectal cancer show an excellent prognosis, a few of them die of metastatic disease. In this subgroup of individuals, the search of occult metastasis might reveal that early dissemination of tumor cells could be the cause of cancer progression. Material and methods. Through a Cancer Registry, we selected all patients with Stage I disease who died of metastatic tumor; a total of 32 patients were identified and in 25 of them paraffin-embedded material was available. The group was matched to 70 Stage I patients with favorable prognosis (controls). In cases and controls resected lymph nodes were cut, and micrometastases were searched using pan-cytokeratin antibodies. Results. Micrometastases were detected in 18 of 25 (72%) Stage I patients who died of the disease, while they were almost absent among controls (1 of 70, p < 0.001 by \u3c72 test). Vascular invasion and tumor budding were more frequent among Stage I patients with an unfavorable prognosis than in controls. By regression analyses, micrometastases (HR 12.3, CI 4.8\u201332) and vascular invasion (HR 3.5, CI 1.4\u20138.5) maintained an independent association with prognosis (cancer-specific survival). Conclusion. Micrometastasis in the lymph nodes can be revealed in the majority of patients with early colorectal cancer who die of tumor progression, while they appear extremely rare in Stage I individuals with good prognosis. The selection of patients through histology (vascular invasion) and search of occult metastatic cells might represent a way to identify individuals who might benefit from adjuvant chemotherapy