Řešení mimořádných událostí v Národním parku Podyjí

Import 20/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Fakulta hornicko-geologická. Institut bezpečnostního inženýrství (547

Household waste management in the Moravian-Silesian region

Import 05/08/2014Cílem této bakalářské práce je analyzovat stav nakládání s komunálním odpadem v Moravskoslezském kraji. Práce je zaměřena především na komunální odpady z obcí, které produkují domácnosti. V první části je popsán vývoj nakládání s komunálním odpadem v Moravskoslezském kraji. Jsou zde také popsány způsoby nakládání s jednotlivými složkami komunálního odpadu a aktuálně platná legislativa v odpadovém hospodářství. V následující části je zhodnocen Plán odpadového hospodářství Moravskoslezského kraje v souvislosti s komunálním odpadem. Dále je na základě zahraničních zkušeností navrženo několik technická opatření vedoucí ve zvýšení míry využití složek komunálního odpadu. Navržená opatření jsou následně podrobena zhodnocení z hlediska finančních nákladů. Na závěr jsou zjištěné poznatky shrnuty s ohledem na zajištění dlouhodobě udržitelného a environmentálně šetrného systému nakládání s komunálním odpadem v Moravskoslezském kraji.The purpose of this bachelor’s thesis is to analyze household waste management in the Moravian-Silesian region. The thesis is primarily focused on municipal solid waste producted by households in the Moravian-Silesian region. In the first part, there is a review of the household waste management development in the Moravian-Silesian region. Subsequently the methods of household waste management as well as respective legal regulations are described. In the next part, an overview of the Waste Management Plan for the Moravian-Silesian region is incorporated. Several technical solutions for increasing household waste recycling are proposed including those based on accomplishments from abroad. These solutions are thoroughly analyzed from an economic point of view. At the end of the thesis there is a summary of assignments aimed at establishing a long-term sustainable and environmentally sound household waste management in the Moravian-Silesian region.Prezenční546 - Institut environmentálního inženýrstvívelmi dobř

Enzybiotics LYSSTAPH-S and LYSDERM-S as Potential Therapeutic Agents for Chronic MRSA Wound Infections

Antibacterial antibiotic therapy has played an important role in the treatment of bacterial infections for almost a century. The increasing resistance of pathogenic bacteria to antibiotics leads to an attempt to use previously neglected antibacterial therapies. Here we provide information on the two recombinantly modified antistaphylococcal enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S) derived from kayvirus 812F1 whose target sites reside in the bacterial cell wall. LYSSTAPH-S showed a stable antimicrobial effect over 24-h testing, even in concentrations lower than 1 µg/mL across a wide variety of epidemiologically important sequence types (STs) of methicillin-resistant Staphylococcus aureus (MRSA), especially in the stationary phase of growth (status comparable to chronic infections). LYSDERM-S showed a less potent antimicrobial effect that lasted only a few hours at concentrations of 15 μg/mL and higher. Our data indicate that these antimicrobial enzymes could be of substantial help in the treatment of chronic MRSA wound infections

Targeted in vivo inhibition of specific protein–protein interactions using recombinant antibodies

With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. This selection method can serve as a prototype for developing unique recombinant antibodies able to interfere with virtually any biomolecule in the living cell

<i>In vitro</i> characterization of scFv hB7A to AHP antigens.

<p>(<b>A</b>) <b>Sequence variability of AHP proteins amino acid sequence.</b> ClustalW tree of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEINS (AHP1-6). Amino acid sequence identity of AHP proteins (compared to AHP3 in brackets). (<b>B</b>) <b>Far-western blot of recombinant AHP proteins.</b> Recombinant protein expression with incorporated 6x-His-tag detected was confirmed by anti-6x-His-tag antibody immunodetection (top); AHP proteins were detected by recombinant scFv hB7A from the periplasmic extract of the bacterial expression, incorporated c-myc-tag detected by anti-c-myc-tag antibody (bottom). (<b>C</b>) <b>The specificity of scFv hB7A against AHP proteins tested in indirect ELISA.</b> Absorbance values of triplicates (±SD represented with error bars) at 450 nm are displayed for each AHP protein (500 ng/well). (<b>D</b>) <b>The affinity of scFv hB7A to AHP3 tested in competitive ELISA.</b> Absorbance values at 405 nm normalized to 490 nm of quadruplicates from two independent measurements were pooled (±SD represented with error bars). The AHP3 was coated at 20 nM and 2-fold dilutions of 1950 nM AHP3 was equilibrated with 45 nM scFv hB7A prior loading to wells. The data was fitted with DYNAFIT software.</p

Recombinant antibody scFv hB7A is interacting with AHP 3 in <i>A. thaliana</i>.

<p>Confocal images of <i>A. thaliana</i> mesophyll protoplasts co-transformed with nYFP:scFv-hB7A (<b>A</b>) and scFv-hB7A:nYFP (<b>B</b>) interacts irrespective of the fusion orientation with AHP3:cYFP (I. - yellow channel). The signal of the reconstituted split-YFP protein is localized in the cytosol and in the nucleus. Nuclear localized mCherry (II. - red channel) and autofluorescence (>650 nm) of chloroplasts (III. - cyan channel) serve as co-localization markers. The integrity of the cell is visible from the overlay picture together with transmission channel (IV.). Schematic representation of the experiment (V.) Negative control is shown in (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109875#pone.0109875.s008" target="_blank">Figure S8</a></b>). Scale bars: 20 µm.</p

Specific down-regulation of the cytokinin signaling pathway in <i>A. thaliana</i> mesophyll protoplasts after ectopic expression of scFv hB7A.

<p>Levels of the TCS:LUC induced luciferase activity correspond to the activity of the cytokinin signaling pathway activated with 100 nM trans-Zeatin (tZ) and normalized to the transfection control 35S:Renilla Luciferase. The ectopic expression of scFv hB7A is sorted by the amount of total (20 µg) co-transfected DNA (expressed in percent). The Col-0 wild type plants (white) and ahp 1,3,4,5 mutant plants with solitary active AHP2 (gray) show no significant effect. The ahp 1,2,4,5 mutant plants (black) with solitary active AHP3 were severely affected</p