Function of zinctransporters in human cells

This study contributes to the knowledge of zinc homeostasis involving members of the ZnT family, in particular ZnT5 and ZnT6, and their role in the molecular basis of an inherited disorder of zinc metabolism in humans, and their function in cultured neuronal cells

Oral and maxillofacial surgery in patients on anticoagulant/antiplatelet therapy- a review

Objective: To review the antiplatelet and anticoagulant drugs currently used and to highlight the management of patients with anticoagulant therapy undergoing minor and invasive oral and maxillofacial surgical procedures. Materials and methods: A systematic review of the literature on PubMed, Science Direct, Springer regarding anticoagulation therapy during oral surgery procedures from 1996 to 2020 was done. Data on the indications for anticoagulant treatment, the regimen of anticoagulation, bridging used, the procedure done, local hemostatic agents used, thromboembolic outcome, follow-up period and bleeding characteristics were collected. Results: The thromboembolic risk due to withdrawal of oral anticoagulants outweighs the risk of bleeding and thus in majority of minor oral surgical procedures discontinuing the regimen is not recommended. Most of the bleeding complications can be controlled with local hemostatic measures. Major invasive oral and maxillofacial procedures with high risk patients warrants cessation of anticoagulant drugs with bridging therapy. The novel oral anticoagulants and newer antiplatelet agents has definite advantages over older main stay drugs especially warfarin and aspirin

A Meta Analysis on Cardiac Vascular Disease with Obesity

Increasing evidence suggests that inflammatory and immunological factors present in the general population play a role in the pathogenesis of cardiovascular disease in RA. More than that, RA is treated with a wide range of drugs, many of which have the potential to alter cardiovascular function. Non-steroidal anti-inflammatory medicines (NSAIDs) have been linked to an increased risk, whereas some treatments, like methotrexate and TNF inhibitors, have been linked to a reduced risk. Cardiac catheterization is just one of several possible procedures that can be done on the heart. More than a million people in the United States get cardiac catheterization each year. [1] Any time a surgeon makes incisions into a patient, they must be ready for the possibility of complications, whether they arise from the patient or the surgery itself. Fortunately, the prevalence of these issues has decreased considerably in recent years thanks to developments in cardiac catheterization equipment, advances in the experience of the operators, and the introduction of novel methods. The term "cardiac catheterization" can refer to either a right heart or a left heart catheterization, or both. Depending on the patient\u27s condition, interventional cardiologists can do either diagnostic or therapeutic operations. The dangers and difficulties that can arise during a diagnostic cardiac catheterization are briefly discussed in this article

Zinc and DHA have opposing effects on the expression levels of histones H3 and H4 in human neuronal cells

Zn and DHA have putative neuroprotective effects and these two essential nutrients are known to interact biochemically. We aimed to identify novel protein candidates that are differentially expressed in human neuronal cell line M17 in response to Zn and DHA that would explain the molecular basis of this interaction. Two-dimensional gel electrophoresis and MS were applied to identify major protein expression changes in the protein lysates of human Ml7 neuronal cells that had been grown in the presence and absence of Zn and DHA. Proteomic findings were further investigated using Western immunoblot and real-time PCR analyses. Four protein spots, which had significant differential expression, were identified and selected for in-gel trypsin digestion followed by matrix-assisted laser desorption ionisation MS analysis. The resultant peptide mass fingerprint for each spot allowed their respective identities to be deduced. Two human histone variants H3 and H4 were identified. Both H3 and H4 were downregulated by Zn in the absence of DHA (Zn effect) and upregulated by DHA (DHA effect) in the presence of Zn (physiological condition). These proteomic findings were further supported by Western immunoblot and real-time PCR analyses using H3- and H4-specific monoclonal antibodies and oligonucleotide primers, respectively. We propose that dietary Zn and DHA cause a global effect on gene expression, which is mediated by histones. Such novel information provides possible clues to the molecular basis of neuroprotection by Zn and DHA that may contribute to the future treatment, prevention and management of neurodegenerative diseases such as Alzheimer\u27s disease.<br /

Antimicrobial Activity of Secondary Metabolites in Medicinal Plants: An Update

Plants have filled in as a significant wellspring of elements for conventional drugs for centuries. Verifiable records and present day ethno botanical field examines feature their significance in the conventional treatment of irresistible malady. Be that as it may, plants form just a minor level of present collection of FDA-endorsed antimicrobial medications. The present article gives an outline of active components of plants as hint for other wellspring of antimicrobial agents to be used in the battle against microscopic organisms. It additionally surveys the ethno botanical way to deal with sedate disclosure and talks about various inventive focuses for future medication revelation endeavours in this field. Without an uncertainty, antimicrobials are miracle tranquilizers. They have represented a very long time against different irresistible infections and spared a huge number of lives. The ongoing disappointment of antimicrobials because of the sensational rise of multidrug-safe microbes and fast spread of new diseases, be that as it may, prompts wellbeing associations and the pharmaceutical business worldwide to change their methodology and to expand improvement of antimicrobials against quickly rising anti-infection safe microorganisms. In spite of the fact that there is thinking about elective wellsprings of characteristic antimicrobial molecules from plants having different methods of activity, some of which have been utilized in regular medication for a considerable length of time and have appeared to have serious impacts contrasted with other antimicrobials. This examination portrays plant intensity as an elective hotspot for antimicrobial agents

The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport

Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.<br /

Design Formulation and Evaluation of Anti Migraine Mouth Dissolving Tablets Using Different Super Disintegrants

The main objective of this research was to create and evaluate the efficacy of orally disintegrating tablets containing sumatriptan succinate at a dosage of 25 milligrammes, a medicine commonly prescribed for the treatment of migraines. The tablets are made using the direct compression method. In order to achieve best results, the formulations were enriched with microcrystalline cellulose of varying composition (Avicel PH 102), mannitol as a diluent, crospovidone, croscaramellose, and sodium starch glycollate as superdisintegrants. Carbomer (carbopol 940), Sodium CMC, and Sodium Alginate were among the other excipients that were used. When used at varying doses, these excipients act as disintegrants. In addition, magnesium stearate was used as a substance to reduce friction, while talc was used as a substance to improve flow. We assessed each of the excipients to ascertain their compatibility with the model drug. The findings revealed no occurrence of any physical or chemical interaction. Before compression, the preformulation features of the tablet blend were examined. The criteria considered were bulk density, tapped density, compressibility index, and hausner ratio. An assessment was carried out on central tablets to ascertain their dimensions, firmness, tendency to crumble, variability in weight, rate of disintegration, and uniformity of drug content properties. Furthermore, an investigation was conducted to examine the impact of these variables on the release of the drug. The drug release studies were performed in vitro using the USP dissolving apparatus-II (paddle type) with a phosphate buffer solution at a pH of 6.8. The experiments were conducted at a speed of 50 revolutions per minute at a temperature of 37 degrees Celsius, with a standard deviation of 5 degrees Celsius. The sampling was conducted at consistent intervals of 2, 4, 6, 8, and 10 minutes. After each withdrawal, an equivalent volume of dissolving medium was replaced with the sample. The ultraviolet (UV) method is employed to evaluate the cumulative quantity of medications that have been discharged at different time intervals. Based on the evaluation results, the F-3 trial formulation, which included 6% crospovidone, was selected as the superior formulation among the superdisintegrants. Conversely, the F-10 trial formulation, which included 2% carbopol 940p, was selected as the superior formulation compared to other basic disintegrants

A Novel Image Super-Resolution Reconstruction Framework Using the AI Technique of Dual Generator Generative Adversarial Network (GAN)

Image superresolution (SR) is the process of enlarging and enhancing a low-resolution image. Image superresolution helps in industrial image enhancement, classification, detection, pattern recognition, surveillance, satellite imaging, medical diagnosis, image analytics, etc. It is of utmost importance to keep the features of the low-resolution image intact while enlarging and enhancing it. In this research paper, a framework is proposed that works in three phases and generates superresolution images while keeping low-resolution image features intact and reducing image blurring and artifacts. In the first phase, image enlargement is done, which enlarges the low-resolution image to the 2x/4x scale using two standard algorithms. The second phase enhances the image using an AI-empowered Generative adversarial network (GAN). We have used a GAN with dual generators and named it EffN-GAN (EfficientNet-GAN). Fusion is done in the last phase, wherein the final improved image is generated by fusing the enlarged image and GAN output image. The fusion phase helps in reducing the artifacts. We have used the DIV2K dataset to train the GAN and further tested the results on the images of Set5, Set14, B100, Urban100, Manga109 datasets with ground truth of size 224x224x3. The obtained results were compared with the state-of-the-art superresolution approach based on important image quality parameters, namely, Peak signal-to--to-noise ratio (PSNR), Structural similarity index (SSIM), Visual information fidelity (VIF) image quality parameters. The results show that the proposed framework for generating super-resolution images from 2x/4x resolution downgraded images improves the aforementioned mentioned image quality parameters significantly

The Influence of Palliative Care Consultation On Health Care Resource Utilization During The Last 2 Months Of Life Report From An Integrated Palliative Care Program And Review Of The Literature

Background: We reviewed 104 consecutive deaths of veterans receiving care in the Dayton VA Medical Center from October 10, 2015 to April 11, 2016. The purpose of our study was to test our hypothesis that palliative care consultation would be associated with reduced health care resource utilization for individuals approaching end of life. Methods: Medical records were reviewed and data entry recorded on a spreadsheet. Non-parametric statistical methods were used to compare four outcome variables from veterans with palliative care consultation (PCC) vs. those without PCC. These variables included the number of ED visits, hospitalizations, hospital days, and ICU days all during the last two months of life. Predictor variables included PCC vs. no PCC and PCC before vs. PCC during the last two months of life. The study sample was comprised of 102 patients after excluding two outlier cases with ethical challenges in surrogate decision-making. Results: Of the 102 consecutive veteran deaths, palliative care consultation was associated with a lower number of ICU days during the last two months of life. For 96 veterans with PCC, the frequency of ED visits and acute care hospitalizations, as well as the number of ICU and hospital days, were all significantly less after PCC compared to before PCC during the last two months of life. The timing of PCC had no effect on the outcomes of interest. Conclusion: Palliative care consultation has a notable effect on health care resource utilization during the last two months of life