MtSNPscore: a combined evidence approach for assessing cumulative impact of mitochondrial variations in disease

Human mitochondrial DNA (mtDNA) variations have been implicated in a broad spectrum of diseases. With over 3000 mtDNA variations reported across databases, establishing pathogenicity of variations in mtDNA is a major challenge. We have designed and developed a comprehensive weighted scoring system (MtSNPscore) for identification of mtDNA variations that can impact pathogenicity and would likely be associated with disease. The criteria for pathogenicity include information available in the literature, predictions made by various in silico tools and frequency of variation in normal and patient datasets. The scoring scheme also assigns scores to patients and normal individuals to estimate the cumulative impact of variations. The method has been implemented in an automated pipeline and has been tested on Indian ataxia dataset (92 individuals), sequenced in this study, and other publicly available mtSNP dataset comprising of 576 mitochondrial genomes of Japanese individuals from six different groups, namely, patients with Parkinson's disease, patients with Alzheimer's disease, young obese males, young non-obese males, and type-2 diabetes patients with or without severe vascular involvement. MtSNPscore, for analysis can extract information from variation data or from mitochondrial DNA sequences. It has a web-interface http://bioinformatics.ccmb.res.in/cgi-bin/snpscore/Mtsnpscore.pl webcite that provides flexibility to update/modify the parameters for estimating pathogenicity

Capacity Analysis of MIMO-WLAN Systems with Single Co-Channel Interference

[[abstract]]In this paper, channel capacity of multiple-input multiple-output wireless local area network (MIMO-WLAN) systems with single co-channel interference (CCI) is calculated. A ray-tracing approach is used to calculate the channel frequency response, which is further used to calculate the corresponding channel capacity. The ability to combat CCI for the MIMO-WLAN simple uniform linear array (ULA) and polarization diversity array (PDA) are investigated. Also the effects caused by two antenna arrays for desired system and CCI are quantified. Numerical results show that MIMO-PDA is better than those of MIMO-ULA when interference is present.[[notice]]補正完畢[[incitationindex]]EI[[booktype]]紙本[[booktype]]電子

Titration curves of interacting cytochrome b5 and hemoglobin by isoelectric focusing-electrophoresis

A strong interaction between cytochrome b5 and hemoglobin has been demonstrated by titration curves in isoelectric focusing - electrophoresis. The pH of maximum interaction is in the pH range 8.0-8.3, which suggests a predominant role of Lys of met hemoglobin in the binding to acidic amino acids of cytochrome b5. The stoichiometry of the complex appears to be 1:1 (cytochrome b5: hemoglobin subunit) with similar binding affinities for \u3b1 and \u3b2 chains

Management of UWB picocell clusters : UCELLS project approach

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